TABLE CONTENTS

Effect of Neem Based Biopesticides on Meloidogyne incognita and
Rotylenchulus reniformis Attacking Tomato
Siddiqui, M.A.
Department of Botany, Aligarh Muslim University, Aligarh-202002, (U.P.) India.
Pot experiments were conducted in two successive years to evaluate the potential of some
neem, Azadirachta indica A. Juss, based biopesticides viz., Achook 0.15%; Neem Raj 0.15%,
Fortune aza 0.15% and Neem drop on the root-knot development caused by Meloidogyne
incognita (Kofoid and White) Chitwood and population of reniform nematode Rotylenchulus
reniformis (Linford and Oliveira), attacking tomato (Lycopersicon esculentum Mill. cv ‘Pusa
Ruby’). These neem based biopesticides were applied @3 ml/pot and all the biopesticides
significantly reduced the nematode multiplication. The highest reduction in nematode
infection was observed in pots treated with neem drop followed by Achook, Neem Raj and
Fortune aza respectively. The reduction in the nematode infection simultaneously enhanced
the plant growth characters, the highest being in neem drop treated pots.
Biocontrol of Meloidogyne incognita using Antagonistic Fungi, Plant
Growth Promoting Rhizobacteria and Composted Cow Manure on Tomato
Siddiqui, Z.A. (1,2) & K. Futai (1)
(1) Graduate School of Agriculture, Faculty of Agriculture, Kyoto University, Kyoto, Sakyo-ku, 606-8502,
Japan; (2) Permanent address: Department of Botany, Aligarh Muslim University, Aligarh-202002, India
The effects of antagonistic fungi (Aspergillus niger, Paecilomyces lilacinus and Penicillium
chrysogenum) and plant growth promoting rhizobacteria (PGPR) (Azotobacter chroococcum,
Bacillus subtilis and Pseudomonas putida) were assessed alone and in combination with
composted cow manure (CCM) on the growth of tomato and on the reproduction of
Meloidogyne incognita in glass house experiments. Applications of antagonistic fungi and
PGPR alone and in combination with CCM caused a significant increase in the growth of
both nematode inoculated and un-inoculated plants. Application of P. lilacinus caused a 38 %
increase in the growth of nematode inoculated plants statistically similar to that caused by P.
putida. Similarly, A. niger caused a 33% increase in the growth of nematode inoculated
plants almost similar to that caused by B. subtilis or A. chroococcum. However, use of P.
chrysogenum was least effective (29%) in increasing growth of nematode inoculated plants.
Moreover, addition of CCM caused a 42 % increase in the growth of nematode inoculated
plants. Highest increase (79%) in the growth of nematode inoculated plants was observed
when P. putida was used with CCM followed by use of P. lilacinus plus CCM (73%).
Inoculation of P. lilacinus caused a high reduction in galling and nematode multiplication
followed by P. putida, B. subtilis, A. niger, CCM, A. chroococcum and P. chrysogenum.
Combined use of P. lilacinus with CCM caused a maximum reduction in galling and
nematode multiplication while use of P. chrysogenum with CCM was the least. Root
colonization by PGPR was increased in the presence of CCM while nematodes had an
adverse effect on root colonization of PGPR. P. lilacinus or P. putida may be used with CCM
for the biocontrol of M. incognita on tomato.
5 th International Congress of Nematology, 2008 287