TABLE CONTENTS

Discovery of Beddingia siricidicola Associated with Sirex noctilio in
Ontario, Canada
Yu, Q. (1), P. DeGroot (2), C. Davis (3), I. Leal (4), W. Ye (5) & R. Bedding (6)
(1) Environmental Health Program (Invertebrate Biodiversity), Agriculture and Agri-food Canada, Ottawa,
Ontario K1A 0C6; (2), (3) Canadian Forest Service, Great Lakes Forestry Centre, NRCan., Sault Ste Marie,
Ontario P6A 2E5; (4) Canadian Forest Service, Pacific Forestry Centre, NRCan., Victoria, B.C. V8Z 1M5; (5)
Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture & Consumer
Services; Raleigh, NC 27607-6465; (6) Cook, ACT, Australia 2614
Nematodes of the European woodwasp, Sirex noctilio, a recent discovered invasive alien
insect species in Ontario, were discovered and identified as Beddingia siricidicola Bedding
(Neotylenchidae) by morphological and molecular methods. Sphaerulariid form of female
reproducing ovoviviparously was found within the haemocoel of the siricid; the juveniles
were successfully reared on several isolates the symbiotic fungus Amylostereum areolatum;
the resulting mycophagous adults were compared with paratypes: the distance between
hemizonid and excretory pore is between 20-50 µm. Attempts were made trying to induce the
infective form from the mycophagous stage. The ribosomal DNA ITS, 18S regions, and
mitochondrion CO1 were PCR amplified and sequenced. The sequences were almost
identical to those of B. siricidicola from Australia.
5 th International Congress of Nematology, 2008 226