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The Effect of Different Levels of Population Densities of Heterodera filipjevi<br />

(Madzhidov, 1981) Stelter, 1984 on Bread Wheat under Microplot<br />

Conditions<br />

Hajihasani, A. (1), Z. Tanha Maafi (2), S. Rezaee (3) & M. Ghalandar (4)<br />

(1) Young Researchers Club, Islamic Azad University, Arak, Iran; (2) Iranian Research Institute of Plant<br />

protection, P.O. Box 1454 Tehran 19395, Iran; (3) Plant Pathology Department, Faculty of Agriculture and<br />

Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran; (4) Agricultural and<br />

Natural Resources Research Center of Markazi, Iran<br />

The three species of cereal cyst nematodes Heterodera avenae Wollenweber, 1924, H.<br />

filipjevi (Madzhidov, 1981) Stelter, 1984 and H. latipons Franklin, 1969 are considered to be<br />

the most economically important species on wheat compared others in the H. avenae<br />

complex. H. filipjevi and H. latipons are widely distributed in Iran, while H. avenae has<br />

limited distribution on wheat. To determine the impact of different levels of population<br />

densities on wheat four levels of population densities of H. filipjevi 2.5, 5, 10 and 20 eggs and<br />

J2s /g -1 soil as initial populations were tested on wheat cultivar Sardari in microplot under<br />

natural field conditons arranged as a Completely Randomized Design replicated seven times.<br />

The results showed increasing the H. filipjevi initial population significantly reduced several<br />

growth parameters of wheat (including plant height, number of tillers, spike height, dry root<br />

weight, aerial dry weight, and grain yield) compared to untreated control. The final<br />

population increased with increasing initial population while the reproduction factor (RF –<br />

final population/initial population) reduced by increasing the initial population but it was still<br />

greater than one in all treatments. The regression analysis showed significant negative<br />

relationship between the initial populations and grain yield, and grain yield was reduced even<br />

at lowest population density and reached a maximum reduction of 47% at population density<br />

of 20 egg and J2s/ g -1 soil.<br />

Destroying Melodogyne chitwoodi in Potato before Processing<br />

Ingham, R.E. & N.M. Wade<br />

Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, USA<br />

Columbia root-knot nematode (Meloidogyne chitwoodi, CRKN) infects potato tubers and<br />

causes quality defects such as galling of the external surface and brown spots surrounding the<br />

egg masses of adult females inside the tuber. Both symptoms are considered quality defects<br />

by the potato industry and can cause crop rejection. Furthermore, CRKN is considered a<br />

quarantined pest by several countries and infested tubers are not allowed entry. For example,<br />

in 2005 57 containers (approximately 1,140 MT) of chipping potatoes from the United States<br />

were denied entry into South Korea due to the presence of CRKN and the potatoes were<br />

returned. This resulted in lost revenue to the growers and a loss of product needed for<br />

processing potato chips in South Korea. Although CRKN is aggressively managed a<br />

probability exists that a small percentage of tubers may be lightly infested. An alternative to<br />

re-exportation of potatoes when low amounts of CRKN are detected is to destroy CRKN in<br />

infested potato tissues before processing. Several procedures were tested as destruction<br />

protocols for eliminating live CRKN from potato tissues by treating either whole tubers or<br />

peels, placing the tissues on a mist chamber and daily examining any recovered nematodes<br />

for viability. Exposure of CRKN–infested tissues to chlorine in the form of bleach at<br />

concentrations up to 25,000 ppm Cl was ineffective. Boiling potato peels and water<br />

5 th International Congress of Nematology, 2008 204

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