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ISSN NO 2320-5407 International Journal <strong>of</strong> Advanced Research (2013), Volume 1, Issue 5, 71-78 Journal homepage: http://www.journalijar.com INTERNATIONAL JOURNAL OF ADVANCED RESEARCH RESEARCH ARTICLE Evaluation <strong>of</strong> Anticancer properties <strong>of</strong> Taxusbaccata and Badri cow urine in mice: Clinicohematological study Ankita Joshi, and R.S. Chauha 1. Post Doctoral Fellow, Cell Culture Laboratory, Institute <strong>of</strong> Biotechnology, G.B. Pant University <strong>of</strong> Agriculture and Technology, Patwadangar-263128, Nainital, Uttarakhand, INDIA. 2. Campus Director, Institute <strong>of</strong> Biotechnology, G.B. Pant University <strong>of</strong> Agriculture and Technology, Patwadangar- 263128, Nainital, Uttarakhand, INDIA. Manuscript Info Manuscript History: Received: 12 June 2013 Final Accepted: 23 June 2013 Published Online: July 2013 Key words: Badricow urine, Taxusbaccata, Mice, Body weight, hematology. Abstract In the present investigation, the anticancerous effect <strong>of</strong> Taxusbacaata and distilled Badri cow urine was studied in mice for clinicohematology and body weight for a period <strong>of</strong> six month at an interval <strong>of</strong> 15 days. The study revealed that the values <strong>of</strong> total leucocyte count (TLC), absolute lymphocyte count (ALC) and absolute neutrophil count (ANC) were significantly increased in the treated groups <strong>of</strong> mice either by CUD alone and in combination withTaxusbaccata extracts. At180th day, it was found that there was an increase in body weight, Hemoglobin content (Hb), total erythrocyte count (TEC), total leucocyte count (TLC), absolute lymphocyte count (ALC) and absolute neutrophil count (ANC) levels in CUD +A treated group as 23%, 23.99%, 41%, 40%, 40.31%, and 40.13%, respectively. This clearly indicate the, increase in vitality and defence mechanism <strong>of</strong> body which in turn helps in further healing <strong>of</strong> cancer. Copy Right, IJAR, 2013,. All rights reserved. Introduction Cancer is a disease involving dynamic changes in genome and is characterized by the uncontrolled, uncoordinated and purposeless proliferation <strong>of</strong> malignant cells and their ability to spread, either by growth in the adjacent tissue through invasion or by implantation at distant sites through metastasis. World cancer report issued by International Agency for Research on Cancer (IARC) reported in 2003 that cancer rate is set to increase at an alarming rate globally. The 5-year relative survival rate for all cancers diagnosed between 1999-2005 is 68%, up from 50% in 1975-1977. (Kinzler and Vogelstein, 2002)(Jemal et al., 2011). In India about 70% <strong>of</strong> population obtains medical help from private practitioners and half <strong>of</strong> those who seek medicinal help obtain it from alternative and traditional medicine (Kumar et al., 2004). Poverty and socioeconomic status are other hurdles in treatment (Pal and Mittal, 2004). American cancer society defines complementary and alternative medicines (CAM) simply as anything which is not conventional (Zollman and Vickers, 1999; Park et al., 2003). There are various CAM used for cancer patient worldwide viz. Herbal medicine, acupuncture, Ayurveda, biological agents, traditional Chinese medicines, meditation and yoga etc. However, use <strong>of</strong> herbs for cancer treatment is very popular throughout the world. Distilled cow urine protects DNA and repairs it rapidly as observed after damage due to pesticides. It protects chromosomal aberrations by mitocycin in human leukocyte. Cow urine helps the lymphocytes to survive and not to commit suicide (apoptosis). Pathogenic effect <strong>of</strong> free radicals are prevented through cow urine therapy. Use <strong>of</strong> cow urine one can get the charm <strong>of</strong> a youth as it prevents the free radicals formation. Taxus baccata, commonly known as THUNER, which is mainly found in high altitude area like, Patwadangar, Nainital India also had anticancer and antiviral properties. It is a small to medium-sized evergreen tree, growing 10-20 m tall, exceptionally up to 28 m. It is relatively slow growing, but can be very longlived, with the maximum recorded trunk diameter <strong>of</strong> 71
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