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Global J. Pharmacol., 4 (1): 41-44, 2010 16 Zone <strong>of</strong> inhibition (mm in diameter) 14 12 10 8 6 4 2 0 Bacillus subtilis Pseudomonas aeruginosa Klebsiella pneumoniae Salmonella typhi 5µl 10µl 15µl Amp* Fig. 1: Antibacterial activity <strong>of</strong> cow urine distillate 14 Zone <strong>of</strong> inhibition (mm in diameter) 12 10 8 6 4 2 0 Aspergillus niger Aspergillus flavus 5µl 10µl 15µl Fig. 2: Antifungal activity <strong>of</strong> cow urine distillate Plate 1: Antibacterial activity <strong>of</strong> CUD against Pathogenic bacteria Plate 2= Antifungal activity <strong>of</strong> CUD against Pathogenic fungi 43
Global J. Pharmacol., 4 (1): 41-44, 2010 Antifungal Activity: Antifungal activity <strong>of</strong> cow urine REFERENCES distillate was analysed against Aspergillus niger and Aspergillus flavus. The investigated results were 1. Shah, E., 1997. Herbal composition in cow urine presented in Table 2, Fig. 2 and Plate 2. When the two distillate. US 5693327. Dec 2. fungal organisms were compared, maximum growth 2. Basham, A.L., 1998. Practices <strong>of</strong> medicine in ancient suppression was observed in Aspergillus niger India in Asian Medical systems: A Comparative (8±0.14, 11.3±1.2 and 12.6±0.04, mm in diameter, study. (Ed) by Charles leslie, Motilal Banarsidass, respectively) than Aspergillus flavus (7.3±0.25, 10±0.26 Delhi, pp: 22. and 11±1.2, mm in diameter, respectively). A similar 3. Sarman Singh, 2001. Cow urine has anti Leshmania result reported by Prashith Kekuda et al. [9] Cow Urine donovani effect in vitro. International J. Cow Sci., Distillate at various concentrations was tested for 1(2): 72-73. antifungal activity. The growth reduction in percentage 4. Sadhale, N., 2004. Vishvallabba Agri History Bulletin was taken into consideration and antifungal effect was 5. Asian agri-History Foundation, Secunderabadevaluated. 5% cow urine distillate was more effective 500009. pp: 134. against Mucor sp. (37.1%) followed by A.oryzae (10.2%) 5. Garg, N., Ashok Kumar and R.S.Chauhan, 2005. and A. niger (5.4%). Effect <strong>of</strong> indigenous cow urine on nutrient utilization It was concluded that the cow urine distillate has <strong>of</strong> white leghorn layers. International J. Cow Sci., antibacterial and antifungal activities the inhibitory 1: 36-38. activity can be used in the control <strong>of</strong> bacteria and fungi 6. Khanuja, S.P.S., 2002. Pharmaceutical composition <strong>of</strong> various origins. The test was done in vitro. Same containing cow urine distillate and an Antibiotic. result may be obtained in vivo also. Now a day’s US patent 6410059. June 25. urinotherapy treatment was developed in the medical 7. Bauer, R.W., M.D.K. Kirby, J.C. Sherris and sectors. Further studies analyze which components are M. Turck, 1966. Antibiotic susceptibility testing by responsible for antimicrobial activity and animal model standard single disc diffusion method. American could reveal antibacterial and antifungal activity <strong>of</strong> cow J. Clinical Pathol., 45: 493-496. urine distillate in vivo. 8. Salil Bose, 1982. Biostatistics in Elementary ACKNOWLEDGMENTS 9. Biophysics. Jytoi Book, Madurai, pp: 127-128. Prashith Kekuda, T.R., R. Kavya, R.M. Shrungashree and S.V. Suchitra, 2007. Antifungal activity <strong>of</strong> cow The authors are thankful to Muthaiyah Research urine distillate. (http://www.microbiocare.com/). Foundation, Thanjavur for <strong>of</strong>fering facilities to carry out this study. 44
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