Molecular Biology Grade Ethanol - Fisher Scientific

Molecular Biology Grade Ethanol product performanceResults have been generated using Agilent Bioanalyzer for DNase and RNase, and protein gel data for protease, to demonstratethe absence of these enzymes in BP2818, Fisher BioReagents Molecular Biology Grade Ethanol.DNase test for BP2818Three lots of BP2818 were tested for the absence of DNase.RNase test for BP2818Three lots of BP2818 were tested for the absence of RNase.Gel-likedata fromBioanalyzerBioanalyzer Lane Identification1. Negative control2. Positive control, 5 U of DNase3 & 4. BP2818, Lot # 15 & 6. BP2818, Lot # 27 & 8. BP2818, Lot # 3Gel-likedata fromBioanalyzerBioanalyzer Lane Identification1. Negative control2. Positive control, 5 U of RNase3 & 4. BP2818, Lot # 15 & 6. BP2818, Lot # 27 & 8. BP2818, Lot # 3DNA fragments arepresent in all three lotsof Molecular BiologyGrade Ethanol as seenin the dark bands shownin Lanes 3 through 8 andmatches negative controlshown in Lane 1.RNA fragments arepresent in all three lotsof Molecular BiologyGrade Ethanol (4000 ntand 2000 nt) as seen inthe dark bands shown inLanes 3 through 8 andmatches negative controlshown in Lane 1.For the positive control in Lane 2, DNA is degraded by thepresence of DNase and is not present compared to thenegative control.ResultThere is no DNase contamination found in any of the three lotsof Ethanol and is shown through the presence of DNA.For the positive control in Lane 2, RNA is degraded by thepresence of RNase and is not present compared to thenegative control.ResultProtease test for BP2818, Fisher BioReagents Molecular Biology Grade EthanolThree lots of BP2818 were tested for the absence of protease.12.5% EZ-Run Protein Gel,BP7712-100 150V for 60 min.Bioanalyzer LaneIdentification1. Negative control2. Positive control, 5 U ofProteinase K enzyme3 & 4. BP2818, Lot # 15 & 6. BP2818, Lot # 27 & 8. BP2818, Lot # 3United KingdomFisher Scientific UKBishop Meadow RoadLoughboroughLeicestershire LE11 5RG+44(0)1509 231166+44(0)1509 231893fsuk.sales@thermofisher.comwww.fisher.co.uk1 2 3 4 5 6 7 8Proteinase K EnzymeThere is no RNase contamination found in any of the three lotsof Ethanol and is shown through the presence of RNA.BSA ProteinBSA protein fragmentsare present in all threelots of Molecular BiologyGrade Ethanol as seenin the dark bands shownin Lanes 3 through 8and matches negativecontrol shown in Lane 1.For the positive control in Lane 2, BSA is degraded bythe Proteinase K enzyme and is not present compared tothe negative control.IrelandFisher Scientific IrelandSuite 3 Plaza 212Blanchardstown CorporatePark 2BallycoolinDublin 15Tel: +353 01 885 5854Fax: +353 01 899 1855fsie.sales@thermofisher.comwww.ie.fishersci.comSwedenFisher ScientificBox 9193400 94 GöteborgTel: +46 31 – 352 32 00Fax: +46 31 – 352 32 50fisher.se@thermofisher.comwww.fishersci.seDenmarkFisher Scientific A/SIndustrivej 3DK-3550 SlangerupTel: +45 70 27 99 20Fax: +45 70 27 99 29kundeservice@thermofisher.comwww.fishersci.dkResultThere is no proteasecontamination found in any ofthe three lots of Ethanol andis shown through the presenceof BSA protein.NorwayFisher ScientificFrysjaveien 33E0884 OsloTel: +47 22 95 59 59Fax: +47 22 95 59 40fisher.no@thermofisher.comwww.fishersci.noFinlandFisher Scientific OyRatastie 2FI-01620 Vantaa+358 802 76 237+358 802 76 235fisher.fi@thermofisher.comwww.fishersci.fi©2010 Thermo Fisher Scientific Inc. All rights reserved. These brands and trademarks are part of Thermo Fisher Scientific Inc. 09_2341 JH/LY PB 2010 33