EEBA Program (PDF/3MB) - EEBA - Annual Meeting
EEBA Program (PDF/3MB) - EEBA - Annual Meeting EEBA Program (PDF/3MB) - EEBA - Annual Meeting
INVITED LECTURE: THOMAS FUCHSLUGER, GERMANY: ARTIFICIALCORNEAHead, LIONS Cornea Bank in North Rhine-WestphaliaConsultant, Department of Ophthalmology at the University Hospital DüsseldorfChair, Cornea Section, European Association for Vision and Eye ResearchBiography: Thomas Fuchsluger, MD FEBO MSc, is a clinician-scientistwith an own reseach group located at the Dept. of Ophthalmology atHeinrich-Heine-University in Düsseldorf, Germany. He is head of theLions Eye Bank at Düsseldorf University Eye Hospital. His research focus is gene and cell therapy tocorneal cells using viral vectors and nanoparticles and ocular surface reconstruction by bioengineered,biocompatible tissues. Since scientific stays in Kyoto/Japan and Harvard Medical School, Boston MA,USA, he gained particular expertise in studying and regulating cell death pathways of corneal cells. Hisgroup is working on translational approaches relating to corneal transplantation, both during cornealstorage and after engraftment. Dr Fuchsluger is associate editor with Acta Ophthalmologica and theOpen Journal of Ophthalmology. Recently, he was elected Chair of the „Cornea & Ocular Surface“section of the European Association for Vision and Eye Research (EVER), the leading ophthalmologicalresearch association in Europe which covers all areas of ophthalmology and the visual sciences.Summary: Artificial corneaArtificial corneas present an alternative to conventional corneal transplantation using corneal tissue.The field can be divided into „classical“ keratoprostheses and „biomimetic“ scaffolds. The presentationwill give an overview over different strategies and recent developments in both groups.70
IDENTIFICATION OF LABEL-RETAINING ENDOTHELIAL CELLS IN ADULT HUMAN CORNEAS: ANEW CLUE FOR THE EXISTENCE OF ENDOTHELIAL STEM CELLSB. M. Ha Thi 1 , Z. He 1 , N. Campolmi 1 , S. Piselli 1 , P. Gain 1 , M. Peoc’h 2 , J. M. Dumollard 2 , S. Acquart 3 , O. Garraud 3 , G. Thuret 1¹Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, Federative Institute of Research in Sciences andHealth Engineering, Faculty of Medicine, Jean Monnet University; ²Department of Pathology, University Hospital ofSaint-Etienne; ³Eye Bank, French Blood Centre, FrancePurpose: The lack of self-renewal capacity of human corneal endothelial cells (EC) in vivo wasexplained by cell cycle arrest in the G1-phase due to cell contact inhibition, TGF-beta signaling, andstress induced premature senescence. Nevertheless, their residual ability to divide in primary culturesuggests the existence of progenitor cells, probably located at the endothelial periphery (Whikehart.MolVis2005; He.StemCells2012). Stem cells are slow-cycling cells characterized by their quiescent statein niches and their ability to retain for a long time markers of S-Phase like BrDU or EdU. Aim: to searchfor the presence of label-retaining EC in human corneas.Methods: Label retaining EC were searched by 5-Ethynyl-2’-Deoxyuridine (Click-it EdU) incorporationduring long-term culture: 30 days for organ cultured human corneas (n=10) or 15 days for in vitroprimary cultured EC (n=5), both followed by a 30-day culture without EdU. Flat-mouted corneas andEC cultures were observed with an inverted fluorescent microscope.Results: label-retaining EC were observed in the peripheral area of all OC corneas, varying from1 to 50. Numerous label-retaining EC were also present in all primary cultures, always attached toDescemet membrane fragments.Conclusions: The presence of label-retaining EC constitutes a new clue for the existence of cornealendothelial stem cells in human. Their apparent scarcity is consistent with the inability of the humancorneal endothelium to repair in vivo, but isolation and expansion of these endothelial stem cells orprogenitors could allow development of bioengineered endothelium.XXV ANNUAL MEETING OF THE EUROPEAN EYE BANK ASSOCIATION Zagreb, Croatia 18/19 January 2013 71
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- Page 25 and 26: Table of contentsINVITED LECTURE: T
- Page 27 and 28: INVITED LECTURE: THOMAS REINHARD, G
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- Page 39 and 40: STUDY OF STROMAL FEMTOSECOND LASER
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- Page 47 and 48: INVITED LECTURE: DONALD TAN, SINGAP
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- Page 51 and 52: A NOVEL OBJECTIVE METHOD TO EVALUAT
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- Page 57 and 58: VISIONGRAFT® STERILE CORNEA - NEW
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- Page 67 and 68: USE OF ANTI-VEGF (BEVACIZUMAB) AFTE
- Page 69: INVITED LECTURE: KEVIN CORCORAN, US
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IDENTIFICATION OF LABEL-RETAINING ENDOTHELIAL CELLS IN ADULT HUMAN CORNEAS: ANEW CLUE FOR THE EXISTENCE OF ENDOTHELIAL STEM CELLSB. M. Ha Thi 1 , Z. He 1 , N. Campolmi 1 , S. Piselli 1 , P. Gain 1 , M. Peoc’h 2 , J. M. Dumollard 2 , S. Acquart 3 , O. Garraud 3 , G. Thuret 1¹Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, Federative Institute of Research in Sciences andHealth Engineering, Faculty of Medicine, Jean Monnet University; ²Department of Pathology, University Hospital ofSaint-Etienne; ³Eye Bank, French Blood Centre, FrancePurpose: The lack of self-renewal capacity of human corneal endothelial cells (EC) in vivo wasexplained by cell cycle arrest in the G1-phase due to cell contact inhibition, TGF-beta signaling, andstress induced premature senescence. Nevertheless, their residual ability to divide in primary culturesuggests the existence of progenitor cells, probably located at the endothelial periphery (Whikehart.MolVis2005; He.StemCells2012). Stem cells are slow-cycling cells characterized by their quiescent statein niches and their ability to retain for a long time markers of S-Phase like BrDU or EdU. Aim: to searchfor the presence of label-retaining EC in human corneas.Methods: Label retaining EC were searched by 5-Ethynyl-2’-Deoxyuridine (Click-it EdU) incorporationduring long-term culture: 30 days for organ cultured human corneas (n=10) or 15 days for in vitroprimary cultured EC (n=5), both followed by a 30-day culture without EdU. Flat-mouted corneas andEC cultures were observed with an inverted fluorescent microscope.Results: label-retaining EC were observed in the peripheral area of all OC corneas, varying from1 to 50. Numerous label-retaining EC were also present in all primary cultures, always attached toDescemet membrane fragments.Conclusions: The presence of label-retaining EC constitutes a new clue for the existence of cornealendothelial stem cells in human. Their apparent scarcity is consistent with the inability of the humancorneal endothelium to repair in vivo, but isolation and expansion of these endothelial stem cells orprogenitors could allow development of bioengineered endothelium.XXV ANNUAL MEETING OF THE EUROPEAN EYE BANK ASSOCIATION Zagreb, Croatia 18/19 January 2013 71
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