EEBA Program (PDF/3MB) - EEBA - Annual Meeting
EEBA Program (PDF/3MB) - EEBA - Annual Meeting EEBA Program (PDF/3MB) - EEBA - Annual Meeting
DNA DAMAGE IN DONOR CORNEAL ENDOTHELIUM UPON TRANSFER FROM OPTISOL GS TOORGAN CULTUREK. Ustgård-Andersen¹, K. Haug¹, A. Azqueta², B. Nicolaissen¹, A. R. Collins¹¹Norwegian Cornea Bank, Center for Eye Research, ¹Department of Ophthalmology, Oslo University Hospital, Ullevaaland University of Oslo; Department of Nutrition, Insitute for Basic Medical Sciences, University of Oslo, NorwayPurpose: The upper limit for storage of donor corneas in Optisol GS is relatively short. Transfer of suchtissue to an Eye Bank Organ Culture (EBOC) system may increase the life span, reduce the numberof donor corneas discarded due to expired shelf life, and increase the overall pool of donor tissue.We have recently examined the limbal epithelium and shown that such transfer is compatible with amaintained regenerative potential and expression of key morphological characteristics (Haug K et al.2012). We here examine the endothelium on donor corneo-scleral rims for DNA damage after primarystorage in Optisol GS and after a subsequent incubation for 1 week in Eye Bank Organ Culture (EBOC).Methods: Comet assay (single cell gel electrophoresis) was used to measure DNA damage in cornealendothelial cells before (n=7) and after (n=5) transferring from cold storage to EBOC. Electrophoresisof lysed and enzyme (FPG, Endo III and T4 Endo V)-treated samples results in structures resemblingcomets, observed by fluorescence microscopy. The intensity of the comet tail relative to the headreflects the frequency of DNA altering lesions (Collins AR & Azqueta A 2012).Results: The level of strand breaks was relatively low in both cold-stored tissue and in the tissueincubated in EBOC for 1 week. Samples retrieved after EBOC showed a significant decrease in FPGsensitivesites reflecting less oxidized purines (mainly 8-hydroxy guanine). Accumulation of dimersinduced by UV-light are indicated by the net T4 endo V-sensitive sites, which is observed to be quitehigh in both groups. The amount of oxidized pyrimidines, given by digestion of Endo III is howeversignificantly decreased upon EBOC incubation.Conclusions: We have found that enzyme-sensitive sites relevant for DNA damage in cornealendothelial cells seem to be decreased using a two-step storage procedure. Our findings may beexplained by a detachment of more severely damaged cells, by less infliction of cellular damage,and also by an activation of DNA repair mechanisms in the more physiological micro-environmentafforded by the EBOC system.56
VISIONGRAFT® STERILE CORNEA – NEW OPTIONS IN OCULAR SURGERYM. Farazdaghi¹, T. E Askew², S. Farazdaghi¹International Federation of Eye & Tissue Banks; ²Tissue Banks International, USAVisionGraft® sterile cornea (Tissue Banks International, Baltimore, Maryland, USA) is a clear, acellular,terminally-sterilized, gamma irradiated allograft with a two year shelf life at room temperature. Thisnovel graft provides a new alternative to addressing ocular surgeries such as corneal ulcers withmicro-perforation, keratoprosthesis – associated corneal melt, limbal mass, pterygium, chemical burns,chronic ulcerative keratitis, glaucoma drainage tube coverage, glaucoma filtration surgery, refractoryglaucoma, etc. Traditionally, treatments of these conditions were managed using fresh corneal tissue,glycerin preserved corneas, sclera, pericardium, amnion or tissue adhesives. VisionGraft® sterile corneacan be used in corneal procedures that do not require a viable endothelium.Histopathology and electron microscopy studies demonstrate similar mean interfibrillar distance ofcollagen fibrils between fresh corneal tissue and VisionGraft® corneas. Light transmission microscopyshows that VisionGraft® corneas are mostly similar to that of fresh corneal tissue. Suture pull studiesdemonstrate comparable tensile strength between the fresh and VisionGraft® corneas.Gamma irradiation offers additional patient safety and virtually eliminates the risk of bacterial or fungaldisease transmission. In addition, gamma irradiation inactivates antigen-presenting cells; functionallyeliminating an immune stimulating allogenic response and reducing rejection potential.VisionGraft® sterile cornea can be used to cover glaucoma draining devices. The tissue remains durableand clear providing for visualization of the drainage tube, therefore provides a treatment window forcorrection of blockage, if necessary. At the same time, it is cosmetically more appealing to the patientcompared to traditionally used sclera and pericardium.This processing method enables use of corneas with low endothelial cell counts thus increasing tissueavailability and utilization of corneas worldwide. VisionGraft® Sterile cornea provides an excellentalternative to use of tissues like, sclera and pericardium and is readily available for trauma andemergency cases. Its use does not require a facility with an eye bank or support from a local eye bankas the tissue can be stored prior to use.XXV ANNUAL MEETING OF THE EUROPEAN EYE BANK ASSOCIATION Zagreb, Croatia 18/19 January 2013 57
- Page 5 and 6: Program OverviewFriday 18 January 2
- Page 9: 16.29 - 16.37 Christina Sanchez Mil
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- Page 25 and 26: Table of contentsINVITED LECTURE: T
- Page 27 and 28: INVITED LECTURE: THOMAS REINHARD, G
- Page 29 and 30: EUROCET 128: SUPPORTING THE IMPLEME
- Page 31 and 32: MENTAL ATTITUDES CONCERNING CORNEA
- Page 33 and 34: AN EVALUATION OF TOTAL BLOOD AND PL
- Page 35 and 36: USE OF FIVE-YEAR CORNEAL GRAFT SURV
- Page 37 and 38: PRECUTTING OF DONOR CORNEAS FOR POS
- Page 39 and 40: STUDY OF STROMAL FEMTOSECOND LASER
- Page 41 and 42: ULTRA- THIN DESCEMET STRIPPING AUTO
- Page 43 and 44: POSTERIOR LAMELLAR KERATOPLASTY- PO
- Page 45 and 46: ENDOTHELIAL CELL DENSITY AFTER DESC
- Page 47 and 48: INVITED LECTURE: DONALD TAN, SINGAP
- Page 49 and 50: INTEGRITY OF HUMAN CORNEAL EPITHELI
- Page 51 and 52: A NOVEL OBJECTIVE METHOD TO EVALUAT
- Page 53 and 54: EVALUATION OF DONOR CORNEAS DURING
- Page 55: DELIVERY OF MOLECULES INTO CORNEAL
- Page 59 and 60: EMERGENCY PREPAREDNESSP. DahlThe Ey
- Page 61 and 62: MANAGEMENT IN NON-TRAUMATIC CORNEAL
- Page 63 and 64: CATARACT AND FUCH’S DYSTROPHY: DS
- Page 65 and 66: RECURRENCE OF ANTERIOR CORNEAL DYST
- Page 67 and 68: USE OF ANTI-VEGF (BEVACIZUMAB) AFTE
- Page 69 and 70: INVITED LECTURE: KEVIN CORCORAN, US
- Page 71 and 72: IDENTIFICATION OF LABEL-RETAINING E
- Page 73 and 74: EPITHELIAL AND PROGENITOR CELL MARK
- Page 75 and 76: INFLUENCE OF STORAGE CONDITIONS OF
- Page 77 and 78: PostersRESULTS OF MORPHOLOGICAL EXA
- Page 79 and 80: KERATOPLASTY “A CHAUD“D. Saric,
- Page 81 and 82: CELL-BY-CELL ALIGNMENT OF REPEATED
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- Page 85 and 86: DEEP ANTERIOR LAMELLAR KERATOPLASTY
- Page 87 and 88: CORECTION OF POST-KERATOPLASTY ASTI
- Page 90: yChoose the highestquality for your
VISIONGRAFT® STERILE CORNEA – NEW OPTIONS IN OCULAR SURGERYM. Farazdaghi¹, T. E Askew², S. Farazdaghi¹International Federation of Eye & Tissue Banks; ²Tissue Banks International, USAVisionGraft® sterile cornea (Tissue Banks International, Baltimore, Maryland, USA) is a clear, acellular,terminally-sterilized, gamma irradiated allograft with a two year shelf life at room temperature. Thisnovel graft provides a new alternative to addressing ocular surgeries such as corneal ulcers withmicro-perforation, keratoprosthesis – associated corneal melt, limbal mass, pterygium, chemical burns,chronic ulcerative keratitis, glaucoma drainage tube coverage, glaucoma filtration surgery, refractoryglaucoma, etc. Traditionally, treatments of these conditions were managed using fresh corneal tissue,glycerin preserved corneas, sclera, pericardium, amnion or tissue adhesives. VisionGraft® sterile corneacan be used in corneal procedures that do not require a viable endothelium.Histopathology and electron microscopy studies demonstrate similar mean interfibrillar distance ofcollagen fibrils between fresh corneal tissue and VisionGraft® corneas. Light transmission microscopyshows that VisionGraft® corneas are mostly similar to that of fresh corneal tissue. Suture pull studiesdemonstrate comparable tensile strength between the fresh and VisionGraft® corneas.Gamma irradiation offers additional patient safety and virtually eliminates the risk of bacterial or fungaldisease transmission. In addition, gamma irradiation inactivates antigen-presenting cells; functionallyeliminating an immune stimulating allogenic response and reducing rejection potential.VisionGraft® sterile cornea can be used to cover glaucoma draining devices. The tissue remains durableand clear providing for visualization of the drainage tube, therefore provides a treatment window forcorrection of blockage, if necessary. At the same time, it is cosmetically more appealing to the patientcompared to traditionally used sclera and pericardium.This processing method enables use of corneas with low endothelial cell counts thus increasing tissueavailability and utilization of corneas worldwide. VisionGraft® Sterile cornea provides an excellentalternative to use of tissues like, sclera and pericardium and is readily available for trauma andemergency cases. Its use does not require a facility with an eye bank or support from a local eye bankas the tissue can be stored prior to use.XXV ANNUAL MEETING OF THE EUROPEAN EYE BANK ASSOCIATION Zagreb, Croatia 18/19 January 2013 57
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