EEBA Program (PDF/3MB) - EEBA - Annual Meeting

EVALUATION OF DONOR CORNEAS DURING STORAGE BY FULL-FIELD OPTICAL COHERENCETOMOGRAPHYV. M. BorderieCentre de Recherche Institut de la Vision, UMR S 968 Inserm / UPMC, UMR 7210 CNRS, FranceAssessment of the donor corneal stroma in eye banks is an important issue. In fact several cornealconditions, such as keratoconus, scars after infectious disease, or refractive surgery, are contraindicationsto the use of donor corneal tissue for penetrating or anterior lamellar keratoplasty. Currenttechniques do not fulfill this issue with the expected efficiency. Gross examination of the donorcornea can only detect severe opacities and slit-lamp examination of the donor tissue is difficult whencorneas are retrieved by in situ excision. In addition post mortem corneal edema makes more difficultthe fine evaluation of the stroma with a slit lamp. Full field optical coherence tomography (OCT) is asafe, non-invasive, and non-destructive process which permits optical in-depth biopsies of gross tissuewithin minutes with a 1-µm 2D and 3D histopathological resolution, and easy exploration, acquisition,and rendering in DICOM format. The donor tissue is examined immersed in its storage medium. Enfacesections of the tissue are acquired and various 2-D or 3-D images can be obtained.Analysis of human donor corneas, either normal or diseased, was performed with the Light-CTScannerR. In normal donor corneas, the epithelium, Bowman layer, keratocytes, stromal lamellae,and Descemet’s membrane could be clearly imaged. Corneas with impaired endothelium functionfeatured stromal edema and subepithelial fibrosis. Stromal scars after infectious keratitis or ocularburns were easily distinguished from non-affected stroma. Application of a freeze probe on normaldonor tissue resulted in loss of the superficial and mid epithelial layers. Defects in Bowman layer wereeasily detected.Full-field OCT appears to be a useful tool to assess the donor corneal stroma during storage. This newtechnology is complementary to conventional assessment of the donor endothelium performed ineye banks.XXV ANNUAL MEETING OF THE EUROPEAN EYE BANK ASSOCIATION Zagreb, Croatia 18/19 January 2013 53