EEBA Program (PDF/3MB) - EEBA - Annual Meeting
EEBA Program (PDF/3MB) - EEBA - Annual Meeting EEBA Program (PDF/3MB) - EEBA - Annual Meeting
PROSPECTIVE CLINICAL EVALUATION OF HYPOTHERMIC VS ORGAN CULTURED CORNEALGRAFTSM. Pauk Gulić, S. Lukačević, N. Miličić, N. Drača, M. Ratković, I. DekarisSpecial Eye Hospital Svjetlost, Department of Ophthalmology, School of Medicine in Rijeka, University of Rijeka, CroatiaPurpose: To compare clinical outcome of corneal transplantation patients using corneas preserved byhypothermic (group 1) or organ culture storage (group 2).Methods: Prospective study included 180 patients who underwent perforating keratoplasty in EyeClinic Svjetlost between March 2009 and April 2012. Patients were divided into two groups accordingto the donor corneal storage. Donor charts were reviewed for: death to preservation time (DP),condition of the epithelium in storage and preservation to surgery time (PS). Follow-up was 6-36months and included graft transparency, best corrected visual acuity, endothelial cell loss and othercomplications. The outcomes in two groups were compared.Results: In 75% transplanted patients donor corneas were perserved in hypotermic storage and 25%patients had their corneas stored in organ culture. Preoperative status of donor tissue from group1 was: epithelial exposure in 28% of corneas, epithelial defect in 4%, epithelial sloughing in 24%,intact epithelium in 45% and DP time was 9,5 (±6) hours. In group 2 there was no epithelial defectsand average DP time was 17 (±6.8) hours. Early epithelial defect (EED) occured in 14,8 % of patientsin group 1 immediately after PK, and none in group 2. EED have transfered to persistent epithelialdefect in 23% of patients. In group 2, visual acuity was better at month 1, but after 6 up to 36 monthsthere was no significant difference. Graft reaction occured in 10,7% of patient in group 1 and 5,7 % ingroup 2. Overall corneal graft survival rate in patients in group 1 was 91,6%, in group 2 97,2% and inpatients with PED 62,5%. Other complications such as postoperative astigmatism, intraocular pressureelevation and cataract formation did not differ at any time between the two investigated groups.Conclusions: Organ cultured donor corneas have lower rate of epithelial problems in a recipient eyes.Final graft outcome, ECD loss and visal outcome are not significantly different between patients withor without EED but in case of PED graft rejection becomes higher.50
A NOVEL OBJECTIVE METHOD TO EVALUATE THE OVERALL QUALITY OF CORNEAL TISSUEUSED FOR COMPARATIVE STUDY BETWEEN TWO HYPOTHERMIC PRESERVATION MEDIAD. Ponzin¹, M. Parekh¹, E. Negura¹, G. Salvalaio¹, S. Ferrari¹, C. Albrecht², D. Fortier², M.-C. Amoureux²¹The Veneto Eye Bank Foundation, Italy; ²Eurobio, FrancePurpose: To demonstrate and validate a new evaluation technique for calculating the overall qualityof the cornea and to check its efficacy in a comparative study for corneas preserved in Optisol - GS anda new formulation Cornea Cold®.Methods: 24 pairs of unsuitable corneas with intact epithelial layer and good morphology wereselected for this study. 12 Right and 12 left corneas (and vice versa) were placed in each medium fora 4 week comparative study to reduce the bias. The study was divided into 4 phases, where phase 1was open and the rest were blinded (masked observers). Endothelial cells were stained with trypanblue and were counted under a light microscope to check the mortality and cell density manually.The endothelial cell density and the mortality were taken in consideration together to measure theviable endothelial cell density. Difference between epithelial and endothelial layer was evaluatedmicroscopically for thickness measurement which was also confirmed later using OCT measurements.A transparency device was used for calculating the degree of transparency. Epithelium was stainedusing trypan blue to check the integrity, morphology and viability. All the above subjective parameterswere converted to objective values for determining the overall quality of the cornea. Statistical analysiswas used to confirm the significant difference.Results: The conversion to objective values from subjective analysis helped to evaluate the qualityof corneas at different time intervals of preservation in different media. Students t-test showedstatistically better results (p
- Page 1 and 2: XXV ANNUAL MEETING OFTHE EUROPEAN E
- Page 3 and 4: IntroductionDear Colleagues and Fri
- Page 5 and 6: Program OverviewFriday 18 January 2
- Page 9: 16.29 - 16.37 Christina Sanchez Mil
- Page 13: 10.08 - 10.16 François Majo¹, M.
- Page 16 and 17: fi fi
- Page 18 and 19: fi fi
- Page 21 and 22: Accommodation & TravelingZagrebThe
- Page 23 and 24: Accommodation & TravelingHow to get
- Page 25 and 26: Table of contentsINVITED LECTURE: T
- Page 27 and 28: INVITED LECTURE: THOMAS REINHARD, G
- Page 29 and 30: EUROCET 128: SUPPORTING THE IMPLEME
- Page 31 and 32: MENTAL ATTITUDES CONCERNING CORNEA
- Page 33 and 34: AN EVALUATION OF TOTAL BLOOD AND PL
- Page 35 and 36: USE OF FIVE-YEAR CORNEAL GRAFT SURV
- Page 37 and 38: PRECUTTING OF DONOR CORNEAS FOR POS
- Page 39 and 40: STUDY OF STROMAL FEMTOSECOND LASER
- Page 41 and 42: ULTRA- THIN DESCEMET STRIPPING AUTO
- Page 43 and 44: POSTERIOR LAMELLAR KERATOPLASTY- PO
- Page 45 and 46: ENDOTHELIAL CELL DENSITY AFTER DESC
- Page 47 and 48: INVITED LECTURE: DONALD TAN, SINGAP
- Page 49: INTEGRITY OF HUMAN CORNEAL EPITHELI
- Page 53 and 54: EVALUATION OF DONOR CORNEAS DURING
- Page 55 and 56: DELIVERY OF MOLECULES INTO CORNEAL
- Page 57 and 58: VISIONGRAFT® STERILE CORNEA - NEW
- Page 59 and 60: EMERGENCY PREPAREDNESSP. DahlThe Ey
- Page 61 and 62: MANAGEMENT IN NON-TRAUMATIC CORNEAL
- Page 63 and 64: CATARACT AND FUCH’S DYSTROPHY: DS
- Page 65 and 66: RECURRENCE OF ANTERIOR CORNEAL DYST
- Page 67 and 68: USE OF ANTI-VEGF (BEVACIZUMAB) AFTE
- Page 69 and 70: INVITED LECTURE: KEVIN CORCORAN, US
- Page 71 and 72: IDENTIFICATION OF LABEL-RETAINING E
- Page 73 and 74: EPITHELIAL AND PROGENITOR CELL MARK
- Page 75 and 76: INFLUENCE OF STORAGE CONDITIONS OF
- Page 77 and 78: PostersRESULTS OF MORPHOLOGICAL EXA
- Page 79 and 80: KERATOPLASTY “A CHAUD“D. Saric,
- Page 81 and 82: CELL-BY-CELL ALIGNMENT OF REPEATED
- Page 83 and 84: RIGID GAS-PERMEABLE CONTACT LENS CO
- Page 85 and 86: DEEP ANTERIOR LAMELLAR KERATOPLASTY
- Page 87 and 88: CORECTION OF POST-KERATOPLASTY ASTI
- Page 90: yChoose the highestquality for your
A NOVEL OBJECTIVE METHOD TO EVALUATE THE OVERALL QUALITY OF CORNEAL TISSUEUSED FOR COMPARATIVE STUDY BETWEEN TWO HYPOTHERMIC PRESERVATION MEDIAD. Ponzin¹, M. Parekh¹, E. Negura¹, G. Salvalaio¹, S. Ferrari¹, C. Albrecht², D. Fortier², M.-C. Amoureux²¹The Veneto Eye Bank Foundation, Italy; ²Eurobio, FrancePurpose: To demonstrate and validate a new evaluation technique for calculating the overall qualityof the cornea and to check its efficacy in a comparative study for corneas preserved in Optisol - GS anda new formulation Cornea Cold®.Methods: 24 pairs of unsuitable corneas with intact epithelial layer and good morphology wereselected for this study. 12 Right and 12 left corneas (and vice versa) were placed in each medium fora 4 week comparative study to reduce the bias. The study was divided into 4 phases, where phase 1was open and the rest were blinded (masked observers). Endothelial cells were stained with trypanblue and were counted under a light microscope to check the mortality and cell density manually.The endothelial cell density and the mortality were taken in consideration together to measure theviable endothelial cell density. Difference between epithelial and endothelial layer was evaluatedmicroscopically for thickness measurement which was also confirmed later using OCT measurements.A transparency device was used for calculating the degree of transparency. Epithelium was stainedusing trypan blue to check the integrity, morphology and viability. All the above subjective parameterswere converted to objective values for determining the overall quality of the cornea. Statistical analysiswas used to confirm the significant difference.Results: The conversion to objective values from subjective analysis helped to evaluate the qualityof corneas at different time intervals of preservation in different media. Students t-test showedstatistically better results (p
Change language