EEBA Program (PDF/3MB) - EEBA - Annual Meeting
EEBA Program (PDF/3MB) - EEBA - Annual Meeting EEBA Program (PDF/3MB) - EEBA - Annual Meeting
SURFACE TOPOGRAPHY AND 3-DIMENSIONAL OPTICAL PROFILING OF FEMTOSECOND ANDNOVEL MECHANICAL MICROKERATOME DISSECTED POSTERIOR HUMAN CORNEAL DISCSFOR DSAEKM. M. Dickman¹, M. P.F.H.L. van Maris², T. T.J.M. Berendschot¹, R. M.M.A. Nuitjs¹¹University Eye Clinic Maastricht, Maastricht University Medical Center; ²Eindhoven University of Technology,Department of Mechanical Engieneering, Materials Technology, Multi Scale Lab; The NetherlandsPurpose: To evaluate and compare the surface topography and roughness of human posteriorcorneal buttons for DSAEK dissected with a femtosecond laser (FS), a novel hands-freeoperator-independent mechanical microkeratome system (MK) and an operator dependent MK.Methods: Eighteen human corneas unsuitable for transplantation were obtained from the EuroCornea Bank (ECB) Beverwijk, The Netherlands. Posterior corneal buttons for DSAEK weredissected with either the Intralase FS60 laser (Abbott Medical Optics, Santa Anna, Ca.) usingoptimized settings, the Moria ALTK operator-dependant MK (Moria, Antony, France) or the GebauerSLc operator-independent MK (Gebauer, Neuhausen, Germany). Dissection using the Gebauer SLc MKand the Moria ALTK MK was performed by experienced eye bank technicians and anexperienced corneal surgeon, respectively. Following dissection, corneas were fixed in a 3.0%Glutaraldehyde solution and examined by a confocal profiler (Sensofar PLu2300, Sensofar,Terrassa, Spain) and by environmental scanning electron microscopy (ESEM). Linear Mixed Modelanalysis was used to quantify the difference in surface roughness between the different techniques.Results: Lamellar dissection was successful in all cases but one, cut with the Moria ALTK MK, equippedwith the 450-µm CB head, due to donor perforation. Confocal profiling was successfulin all cases allowing quantitative surface roughness analysis and 3-dimensional reconstruction of thecentral 2x1.5 mm2 area of the dissected grafts. Complimentary ESEM imaging allowed wide fieldanalysis of all samples at lower magnification. Surface roughness of FS60 dissected tissues wassignificantly higher (P < 0.001) than that of both operator-dependent and independent mechanicallydissected tissues, despite optimized laser settings. No significant difference in surface roughness wasfound between operator-dependent and independent dissection although different surfacetopographies were observed with both techniques.Conclusions: Surface roughness of FS60 dissected corneal buttons for DSAEK was significantly higherthan that of mechanically dissected tissues, despite optimized laser settings. No significant differencein surface roughness was found between operator-dependent and independentmechanical dissection. Confocal profiling, a novel imaging modality used in this study for the first timefor quantitative stromal bed roughness analysis, enables non-contact submicron measurements and3-dimensional reconstruction of large clinically significant areas. Future clinical use of this technologycould be useful in the diagnosis and follow up of corneal pathologies.40
ULTRA- THIN DESCEMET STRIPPING AUTOMATED ENDOTHELIAL KERATOPLASTY-FIRSTRESULTSI. Dekaris, S. Lukačević, M. Pauk Gulić, N. MiličićSpecial Eye Hospital Svjetlost, Department of Ophthalmology, School of Medicine in Rijeka, University of Rijeka, CroatiaPurpose: To present our results of Ultra-thin Descemet stripping automated endothelial keratoplasty(UT-DSAEK) in eyes with pseudophakic bullous keratoplasty (PBK) and compare these results toconventional DSAEK.Methods: A prospective case series of 10 PBK eyes undergoing UT DSAEK (group 1) and 30 PBK eyesundergoing conventional DSAEK (group 2) for the treatment of PBK. Both surgeon-cut and „pre-cut“tissue obtained from certified eye banks was used. All patients underwent serial central graft thicknessmeasurements with non-contact optical coherence tomography (Zeiss Visante AS-OCT) at varioustime points after surgery. The eyes in DSAEK group were subdivided into 3 subgroups based on a 1stday postoperative endothelial graft thickness: 2a) thin grafts (thickness:250μm). Differences between the groups regardingbest spectacle-corrected visual acuity (BSCVA) and endothelial cells density loss (ECD) were recorded.Results: There was no statistically significant difference in age, sex, or preoperative BSCVA betweengroups. The median postoperative graft thickness in group 1 was 78±21.30 μm and in group 2 itwas 190±47.61 μm. Postoperative follow-up was 3-18 months. UT DSAEK group showed betterpostoperative BCVA both in quantity (≥0.8) and speed of recovery ( at 1 month) as compared toall conventional DSAEK groups. Thin DSAEK grafts (
- Page 1 and 2: XXV ANNUAL MEETING OFTHE EUROPEAN E
- Page 3 and 4: IntroductionDear Colleagues and Fri
- Page 5 and 6: Program OverviewFriday 18 January 2
- Page 9: 16.29 - 16.37 Christina Sanchez Mil
- Page 13: 10.08 - 10.16 François Majo¹, M.
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- Page 21 and 22: Accommodation & TravelingZagrebThe
- Page 23 and 24: Accommodation & TravelingHow to get
- Page 25 and 26: Table of contentsINVITED LECTURE: T
- Page 27 and 28: INVITED LECTURE: THOMAS REINHARD, G
- Page 29 and 30: EUROCET 128: SUPPORTING THE IMPLEME
- Page 31 and 32: MENTAL ATTITUDES CONCERNING CORNEA
- Page 33 and 34: AN EVALUATION OF TOTAL BLOOD AND PL
- Page 35 and 36: USE OF FIVE-YEAR CORNEAL GRAFT SURV
- Page 37 and 38: PRECUTTING OF DONOR CORNEAS FOR POS
- Page 39: STUDY OF STROMAL FEMTOSECOND LASER
- Page 43 and 44: POSTERIOR LAMELLAR KERATOPLASTY- PO
- Page 45 and 46: ENDOTHELIAL CELL DENSITY AFTER DESC
- Page 47 and 48: INVITED LECTURE: DONALD TAN, SINGAP
- Page 49 and 50: INTEGRITY OF HUMAN CORNEAL EPITHELI
- Page 51 and 52: A NOVEL OBJECTIVE METHOD TO EVALUAT
- Page 53 and 54: EVALUATION OF DONOR CORNEAS DURING
- Page 55 and 56: DELIVERY OF MOLECULES INTO CORNEAL
- Page 57 and 58: VISIONGRAFT® STERILE CORNEA - NEW
- Page 59 and 60: EMERGENCY PREPAREDNESSP. DahlThe Ey
- Page 61 and 62: MANAGEMENT IN NON-TRAUMATIC CORNEAL
- Page 63 and 64: CATARACT AND FUCH’S DYSTROPHY: DS
- Page 65 and 66: RECURRENCE OF ANTERIOR CORNEAL DYST
- Page 67 and 68: USE OF ANTI-VEGF (BEVACIZUMAB) AFTE
- Page 69 and 70: INVITED LECTURE: KEVIN CORCORAN, US
- Page 71 and 72: IDENTIFICATION OF LABEL-RETAINING E
- Page 73 and 74: EPITHELIAL AND PROGENITOR CELL MARK
- Page 75 and 76: INFLUENCE OF STORAGE CONDITIONS OF
- Page 77 and 78: PostersRESULTS OF MORPHOLOGICAL EXA
- Page 79 and 80: KERATOPLASTY “A CHAUD“D. Saric,
- Page 81 and 82: CELL-BY-CELL ALIGNMENT OF REPEATED
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- Page 85 and 86: DEEP ANTERIOR LAMELLAR KERATOPLASTY
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SURFACE TOPOGRAPHY AND 3-DIMENSIONAL OPTICAL PROFILING OF FEMTOSECOND ANDNOVEL MECHANICAL MICROKERATOME DISSECTED POSTERIOR HUMAN CORNEAL DISCSFOR DSAEKM. M. Dickman¹, M. P.F.H.L. van Maris², T. T.J.M. Berendschot¹, R. M.M.A. Nuitjs¹¹University Eye Clinic Maastricht, Maastricht University Medical Center; ²Eindhoven University of Technology,Department of Mechanical Engieneering, Materials Technology, Multi Scale Lab; The NetherlandsPurpose: To evaluate and compare the surface topography and roughness of human posteriorcorneal buttons for DSAEK dissected with a femtosecond laser (FS), a novel hands-freeoperator-independent mechanical microkeratome system (MK) and an operator dependent MK.Methods: Eighteen human corneas unsuitable for transplantation were obtained from the EuroCornea Bank (ECB) Beverwijk, The Netherlands. Posterior corneal buttons for DSAEK weredissected with either the Intralase FS60 laser (Abbott Medical Optics, Santa Anna, Ca.) usingoptimized settings, the Moria ALTK operator-dependant MK (Moria, Antony, France) or the GebauerSLc operator-independent MK (Gebauer, Neuhausen, Germany). Dissection using the Gebauer SLc MKand the Moria ALTK MK was performed by experienced eye bank technicians and anexperienced corneal surgeon, respectively. Following dissection, corneas were fixed in a 3.0%Glutaraldehyde solution and examined by a confocal profiler (Sensofar PLu2300, Sensofar,Terrassa, Spain) and by environmental scanning electron microscopy (ESEM). Linear Mixed Modelanalysis was used to quantify the difference in surface roughness between the different techniques.Results: Lamellar dissection was successful in all cases but one, cut with the Moria ALTK MK, equippedwith the 450-µm CB head, due to donor perforation. Confocal profiling was successfulin all cases allowing quantitative surface roughness analysis and 3-dimensional reconstruction of thecentral 2x1.5 mm2 area of the dissected grafts. Complimentary ESEM imaging allowed wide fieldanalysis of all samples at lower magnification. Surface roughness of FS60 dissected tissues wassignificantly higher (P < 0.001) than that of both operator-dependent and independent mechanicallydissected tissues, despite optimized laser settings. No significant difference in surface roughness wasfound between operator-dependent and independent dissection although different surfacetopographies were observed with both techniques.Conclusions: Surface roughness of FS60 dissected corneal buttons for DSAEK was significantly higherthan that of mechanically dissected tissues, despite optimized laser settings. No significant differencein surface roughness was found between operator-dependent and independentmechanical dissection. Confocal profiling, a novel imaging modality used in this study for the first timefor quantitative stromal bed roughness analysis, enables non-contact submicron measurements and3-dimensional reconstruction of large clinically significant areas. Future clinical use of this technologycould be useful in the diagnosis and follow up of corneal pathologies.40
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