EEBA Program (PDF/3MB) - EEBA - Annual Meeting

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EVALUATION OF THE DECONTAMINATION METHODS OF DONOR CORNEAL. Giurgola¹, R. Mistó², F. Pateri², C. Gatto¹, J. D’Amato Tothova¹¹Research and Development Department of Alchimia S.r.l.; ²Azienda Ospedaliera San Gerardo, ItalyPurpose: Our previous studies showed that standard corneal storage media do not guaranteeefficient decontamination of donor cornea. The aim of the study was to determine the processconditions for effective decontamination of donor corneas.Methods: Thirty donor corneas were procured by the Monza Eye Bank (Italy). Ten corneas were storedin a decontamination medium prototype A at 31°C for 20 days. Twenty corneas were decontaminatedat 4°C overnight in a decontamination solution prototype B and subsequently stored either inEusol-C at 4°C or Tissue-C at 31°C. The decontamination phase was skipped for 24 corneas used ascontrol tissues, which were stored under organ culture conditions. Microbiological analyses wereperformed pre-processing and post-processing after removal of antibiotic residues with the ResEPdevice (ALCHIMIA, Italy). Endothelial cell density (ECD), endothelial morphology and mortality weremonitored pre-processing, 24h post-decontamination and post-processing. Antibiotic residues in thecorneal tissue were determined by HPLC after processing.Results: Pre-processing, 50% of the tissues stored in decontamination medium A were contaminated(Staphylococcus spp, E. Coli, C. Albicans); all tissues resulted decontaminated at the end of the process.The corneas showed unvaried mortality till the end of the storage; an altered endothelial morphologyand ECD reduction were observed starting from the 14th day as compared to controls.54% of the tissues decontaminated at 4°C overnight with decontamination solution B and then storedeither in Eusol-C or in Tissue-C was contaminated pre-processing (Staphylococcus spp.); all tissuesresulted decontaminated at the end of the process. ECD, endothelial morphology and mortality rateresulted unvaried 24h after decontamination and at the end of the process both for organ culture andcold storage.Control tissues, which were stored under organ culture conditions, showed 75% of contaminationpre-processing and 45% contamination at the end of the process. HPLC analysis showed the absenceof antibiotic residues in all investigated tissues at the end of the process.Conclusions: Overnight decontamination at 4°C using decontamination solution B allowed toeliminate all contaminants from donor corneas without tissue alteration. These process conditionsresulted compatible both with organ culture and cold storage.34
USE OF FIVE-YEAR CORNEAL GRAFT SURVIVAL FOR THE VALIDATION OF EYE BANK QUALITYSTANDARDSJ. Armitage¹, M. Jones², I. Zambrano³, F. Carley³, D. Tole¹¹School of Clinical Sciences, University of Bristol, Bristol Eye Hospital; ²NHS Blood & Transplant; ³CTS Manchester EyeBank, Manchester Royal Eye Hospital, UKPurpose: Analysis of the influence of donor and recipient factors on five-year graft survival forvalidation of the quality standards applied in the CTS Eye Banks in Bristol and Manchester.Methods: Corneas stored by the CTS Eye Banks between April 1999 and March 2005 were includedin the study. First, a logistic regression analysis was carried out to determine the influence of donorfactors on the suitability of corneas for penetrating keratoplasty (PK). Only one cornea randomlyselected from each donor was included in this analysis. For corneas in this cohort that were assessedas suitable and transplanted, the influence of donor and recipient factors on five-year graft survival offirst PK was investigated. Survival data were analysed by univariate methods (Kaplan-Meier survival)and multiple regression (Cox proportional hazards), as appropriate.Results: Suitability for PK (n=7107). Donor age (p
Page 1 and 2: XXV ANNUAL MEETING OFTHE EUROPEAN E
Page 3 and 4: IntroductionDear Colleagues and Fri
Page 5 and 6: Program OverviewFriday 18 January 2
Page 9: 16.29 - 16.37 Christina Sanchez Mil
Page 13: 10.08 - 10.16 François Majo¹, M.
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Page 21 and 22: Accommodation & TravelingZagrebThe
Page 23 and 24: Accommodation & TravelingHow to get
Page 25 and 26: Table of contentsINVITED LECTURE: T
Page 27 and 28: INVITED LECTURE: THOMAS REINHARD, G
Page 29 and 30: EUROCET 128: SUPPORTING THE IMPLEME
Page 31 and 32: MENTAL ATTITUDES CONCERNING CORNEA
Page 33: AN EVALUATION OF TOTAL BLOOD AND PL
Page 37 and 38: PRECUTTING OF DONOR CORNEAS FOR POS
Page 39 and 40: STUDY OF STROMAL FEMTOSECOND LASER
Page 41 and 42: ULTRA- THIN DESCEMET STRIPPING AUTO
Page 43 and 44: POSTERIOR LAMELLAR KERATOPLASTY- PO
Page 45 and 46: ENDOTHELIAL CELL DENSITY AFTER DESC
Page 47 and 48: INVITED LECTURE: DONALD TAN, SINGAP
Page 49 and 50: INTEGRITY OF HUMAN CORNEAL EPITHELI
Page 51 and 52: A NOVEL OBJECTIVE METHOD TO EVALUAT
Page 53 and 54: EVALUATION OF DONOR CORNEAS DURING
Page 55 and 56: DELIVERY OF MOLECULES INTO CORNEAL
Page 57 and 58: VISIONGRAFT® STERILE CORNEA - NEW
Page 59 and 60: EMERGENCY PREPAREDNESSP. DahlThe Ey
Page 61 and 62: MANAGEMENT IN NON-TRAUMATIC CORNEAL
Page 63 and 64: CATARACT AND FUCH’S DYSTROPHY: DS
Page 65 and 66: RECURRENCE OF ANTERIOR CORNEAL DYST
Page 67 and 68: USE OF ANTI-VEGF (BEVACIZUMAB) AFTE
Page 69 and 70: INVITED LECTURE: KEVIN CORCORAN, US
Page 71 and 72: IDENTIFICATION OF LABEL-RETAINING E
Page 73 and 74: EPITHELIAL AND PROGENITOR CELL MARK
Page 75 and 76: INFLUENCE OF STORAGE CONDITIONS OF
Page 77 and 78: PostersRESULTS OF MORPHOLOGICAL EXA
Page 79 and 80: KERATOPLASTY “A CHAUD“D. Saric,
Page 81 and 82: CELL-BY-CELL ALIGNMENT OF REPEATED
Page 83 and 84: RIGID GAS-PERMEABLE CONTACT LENS CO
Page 85 and 86:
DEEP ANTERIOR LAMELLAR KERATOPLASTY
Page 87 and 88:
CORECTION OF POST-KERATOPLASTY ASTI
Page 90:
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