EEBA Program (PDF/3MB) - EEBA - Annual Meeting
EEBA Program (PDF/3MB) - EEBA - Annual Meeting EEBA Program (PDF/3MB) - EEBA - Annual Meeting
EVALUATION OF THE DECONTAMINATION METHODS OF DONOR CORNEAL. Giurgola¹, R. Mistó², F. Pateri², C. Gatto¹, J. D’Amato Tothova¹¹Research and Development Department of Alchimia S.r.l.; ²Azienda Ospedaliera San Gerardo, ItalyPurpose: Our previous studies showed that standard corneal storage media do not guaranteeefficient decontamination of donor cornea. The aim of the study was to determine the processconditions for effective decontamination of donor corneas.Methods: Thirty donor corneas were procured by the Monza Eye Bank (Italy). Ten corneas were storedin a decontamination medium prototype A at 31°C for 20 days. Twenty corneas were decontaminatedat 4°C overnight in a decontamination solution prototype B and subsequently stored either inEusol-C at 4°C or Tissue-C at 31°C. The decontamination phase was skipped for 24 corneas used ascontrol tissues, which were stored under organ culture conditions. Microbiological analyses wereperformed pre-processing and post-processing after removal of antibiotic residues with the ResEPdevice (ALCHIMIA, Italy). Endothelial cell density (ECD), endothelial morphology and mortality weremonitored pre-processing, 24h post-decontamination and post-processing. Antibiotic residues in thecorneal tissue were determined by HPLC after processing.Results: Pre-processing, 50% of the tissues stored in decontamination medium A were contaminated(Staphylococcus spp, E. Coli, C. Albicans); all tissues resulted decontaminated at the end of the process.The corneas showed unvaried mortality till the end of the storage; an altered endothelial morphologyand ECD reduction were observed starting from the 14th day as compared to controls.54% of the tissues decontaminated at 4°C overnight with decontamination solution B and then storedeither in Eusol-C or in Tissue-C was contaminated pre-processing (Staphylococcus spp.); all tissuesresulted decontaminated at the end of the process. ECD, endothelial morphology and mortality rateresulted unvaried 24h after decontamination and at the end of the process both for organ culture andcold storage.Control tissues, which were stored under organ culture conditions, showed 75% of contaminationpre-processing and 45% contamination at the end of the process. HPLC analysis showed the absenceof antibiotic residues in all investigated tissues at the end of the process.Conclusions: Overnight decontamination at 4°C using decontamination solution B allowed toeliminate all contaminants from donor corneas without tissue alteration. These process conditionsresulted compatible both with organ culture and cold storage.34
USE OF FIVE-YEAR CORNEAL GRAFT SURVIVAL FOR THE VALIDATION OF EYE BANK QUALITYSTANDARDSJ. Armitage¹, M. Jones², I. Zambrano³, F. Carley³, D. Tole¹¹School of Clinical Sciences, University of Bristol, Bristol Eye Hospital; ²NHS Blood & Transplant; ³CTS Manchester EyeBank, Manchester Royal Eye Hospital, UKPurpose: Analysis of the influence of donor and recipient factors on five-year graft survival forvalidation of the quality standards applied in the CTS Eye Banks in Bristol and Manchester.Methods: Corneas stored by the CTS Eye Banks between April 1999 and March 2005 were includedin the study. First, a logistic regression analysis was carried out to determine the influence of donorfactors on the suitability of corneas for penetrating keratoplasty (PK). Only one cornea randomlyselected from each donor was included in this analysis. For corneas in this cohort that were assessedas suitable and transplanted, the influence of donor and recipient factors on five-year graft survival offirst PK was investigated. Survival data were analysed by univariate methods (Kaplan-Meier survival)and multiple regression (Cox proportional hazards), as appropriate.Results: Suitability for PK (n=7107). Donor age (p
- Page 1 and 2: XXV ANNUAL MEETING OFTHE EUROPEAN E
- Page 3 and 4: IntroductionDear Colleagues and Fri
- Page 5 and 6: Program OverviewFriday 18 January 2
- Page 9: 16.29 - 16.37 Christina Sanchez Mil
- Page 13: 10.08 - 10.16 François Majo¹, M.
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- Page 21 and 22: Accommodation & TravelingZagrebThe
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- Page 25 and 26: Table of contentsINVITED LECTURE: T
- Page 27 and 28: INVITED LECTURE: THOMAS REINHARD, G
- Page 29 and 30: EUROCET 128: SUPPORTING THE IMPLEME
- Page 31 and 32: MENTAL ATTITUDES CONCERNING CORNEA
- Page 33: AN EVALUATION OF TOTAL BLOOD AND PL
- Page 37 and 38: PRECUTTING OF DONOR CORNEAS FOR POS
- Page 39 and 40: STUDY OF STROMAL FEMTOSECOND LASER
- Page 41 and 42: ULTRA- THIN DESCEMET STRIPPING AUTO
- Page 43 and 44: POSTERIOR LAMELLAR KERATOPLASTY- PO
- Page 45 and 46: ENDOTHELIAL CELL DENSITY AFTER DESC
- Page 47 and 48: INVITED LECTURE: DONALD TAN, SINGAP
- Page 49 and 50: INTEGRITY OF HUMAN CORNEAL EPITHELI
- Page 51 and 52: A NOVEL OBJECTIVE METHOD TO EVALUAT
- Page 53 and 54: EVALUATION OF DONOR CORNEAS DURING
- Page 55 and 56: DELIVERY OF MOLECULES INTO CORNEAL
- Page 57 and 58: VISIONGRAFT® STERILE CORNEA - NEW
- Page 59 and 60: EMERGENCY PREPAREDNESSP. DahlThe Ey
- Page 61 and 62: MANAGEMENT IN NON-TRAUMATIC CORNEAL
- Page 63 and 64: CATARACT AND FUCH’S DYSTROPHY: DS
- Page 65 and 66: RECURRENCE OF ANTERIOR CORNEAL DYST
- Page 67 and 68: USE OF ANTI-VEGF (BEVACIZUMAB) AFTE
- Page 69 and 70: INVITED LECTURE: KEVIN CORCORAN, US
- Page 71 and 72: IDENTIFICATION OF LABEL-RETAINING E
- Page 73 and 74: EPITHELIAL AND PROGENITOR CELL MARK
- Page 75 and 76: INFLUENCE OF STORAGE CONDITIONS OF
- Page 77 and 78: PostersRESULTS OF MORPHOLOGICAL EXA
- Page 79 and 80: KERATOPLASTY “A CHAUD“D. Saric,
- Page 81 and 82: CELL-BY-CELL ALIGNMENT OF REPEATED
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EVALUATION OF THE DECONTAMINATION METHODS OF DONOR CORNEAL. Giurgola¹, R. Mistó², F. Pateri², C. Gatto¹, J. D’Amato Tothova¹¹Research and Development Department of Alchimia S.r.l.; ²Azienda Ospedaliera San Gerardo, ItalyPurpose: Our previous studies showed that standard corneal storage media do not guaranteeefficient decontamination of donor cornea. The aim of the study was to determine the processconditions for effective decontamination of donor corneas.Methods: Thirty donor corneas were procured by the Monza Eye Bank (Italy). Ten corneas were storedin a decontamination medium prototype A at 31°C for 20 days. Twenty corneas were decontaminatedat 4°C overnight in a decontamination solution prototype B and subsequently stored either inEusol-C at 4°C or Tissue-C at 31°C. The decontamination phase was skipped for 24 corneas used ascontrol tissues, which were stored under organ culture conditions. Microbiological analyses wereperformed pre-processing and post-processing after removal of antibiotic residues with the ResEPdevice (ALCHIMIA, Italy). Endothelial cell density (ECD), endothelial morphology and mortality weremonitored pre-processing, 24h post-decontamination and post-processing. Antibiotic residues in thecorneal tissue were determined by HPLC after processing.Results: Pre-processing, 50% of the tissues stored in decontamination medium A were contaminated(Staphylococcus spp, E. Coli, C. Albicans); all tissues resulted decontaminated at the end of the process.The corneas showed unvaried mortality till the end of the storage; an altered endothelial morphologyand ECD reduction were observed starting from the 14th day as compared to controls.54% of the tissues decontaminated at 4°C overnight with decontamination solution B and then storedeither in Eusol-C or in Tissue-C was contaminated pre-processing (Staphylococcus spp.); all tissuesresulted decontaminated at the end of the process. ECD, endothelial morphology and mortality rateresulted unvaried 24h after decontamination and at the end of the process both for organ culture andcold storage.Control tissues, which were stored under organ culture conditions, showed 75% of contaminationpre-processing and 45% contamination at the end of the process. HPLC analysis showed the absenceof antibiotic residues in all investigated tissues at the end of the process.Conclusions: Overnight decontamination at 4°C using decontamination solution B allowed toeliminate all contaminants from donor corneas without tissue alteration. These process conditionsresulted compatible both with organ culture and cold storage.34
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