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AN EVALUATION OF TOTAL BLOOD AND PLASMA VOLUME CALCULATIONS IN EYE ANDTISSUE BANKINGC. Sanchez MillerInternational Sight Restoration Eye Bank, Florida, USAPurpose: To evaluate the differences of weight-based total blood and plasma volumes compared tothe Nadler Total Blood Volume Formula and a percentage-based plasma volume calculation.Methods: Review of 58 adult donors and 1 pediatric donor from ISR Eye Bank. The gender, weightin kilograms, and height in meters was obtained from each donor. Each donor was classified asoverweight and normal according to the Body Mass Index, with the exception of the pediatric case.The total blood volume was calculated for each donor using the standard weight-based (.07L/kg) totalblood volume estimation and the Nadler Formula. The total plasma volume was calculated for eachdonor using the standard weight-based (.04L/kg) total plasma volume estimation and a percentagebased(55%) plasma volume estimation. The averages of the total blood and plasma volumes for alldonors, overweight donors, and normal donors were calculated. The ranges of total blood and plasmavolumes were determined for all donors, overweight donors, and normal donors.Results: There were 41 overweight donors and 17 normal donors that comprised the study. Thepediatric case was not classified as overweight or normal. The average total blood volume for alldonors utilizing the weight-based calculation was 6.63L compared to the Nadler Formula whichaveraged 5.56L. The two calculations yielded an average difference of 1.07L. The average total bloodvolume for overweight donors utilizing the weight-based calculation was 7.35L compared to theNadler Formula which averaged 5.91L. The two calculations yielded an average difference of 1.44L.The average total blood volume for normal donors utilizing the weight-based calculation was 4.92Lcompared to the Nadler Formula which averaged 4.72L. The two calculations yielded an averagedifference of 0.20L. The range of the weight-based calculation and Nadler Formula for total bloodvolume was 4.14L-14.28L and 3.43L-8.90L, respectively. The average total plasma volume for all donorsutilizing the weight-based calculation was 3.79L compared to the percentage-based calculationwhich averaged 3.06L. The two calculations yielded an average difference of 0.73L. The average totalplasma volume for overweight donors utilizing the weight-based calculation was 4.20L comparedto the percentage-based calculation which averaged 3.25L. The two calculations yielded an averagedifference of 0.95L. The average total plasma volume for normal donors utilizing the weight-basedcalculation was 2.81L compared to the percentage-based calculation which averaged 2.60L. The twocalculations yielded an average difference of 0.21L. The range of the weight-based calculation andpercentage-based calculation for total plasma volume was 2.29L-8.16L and 1.89L-4.89L, respectively.The pediatric case yielded a total blood volume of 1.27L for the weight-based calculation and 1.19L forthe Nadler Formula. The total plasma volume for the weight-based calculation was of 0.73L and 0.66Lfor the percentage-based calculation.Conclusions: Among the classes of all and overweight donors, the weight-based calculation fortotal blood volume and total plasma volume was over-estimated. There was no significant differenceamong the normal donors. The Nadler Formula and the percentage-based calculation are best suitedfor estimating blood and plasma volumes for all adult donors, regardless of weight classification. Allcalculations for the pediatric case were not supported by literature. Calculations of blood and plasmavolumes for pediatric donors need further evaluation. When calculating total blood and plasmavolumes for plasma dilution algorithms, eye and tissue banks should use stricter formulas resulting inlower volumes. Lower volumes are less likely to result in false negatives due to plasma diluted samples.XXV ANNUAL MEETING OF THE EUROPEAN EYE BANK ASSOCIATION Zagreb, Croatia 18/19 January 2013 33