Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

More documents

Recommendations

Info

Open gut cavity. Photograph internal organs, noting any abnormalities Liver weighed, then divided into two samples:o Sample for chemical residue testing – frozen. This should consist of 2aliquots each containing at least 10 g (where possible)o Sample for histology - ~1 cm cubed in 50 ml 10% formalin Other tissues for histology: heart, spleen, kidney, hindgut - ~1 cm cubed fixed in100ml 10% formalin Gonad status, bile appearance, mesentery fat and parasite load were evaluatedaccording to the data sheet categories and any abnormalities noted Muscle sample taken –o Sample for residue testing – frozen. This should consist of 2 aliquots eachcontaining at least 10 g (where possible)o Sample for histology - ~1 cm cubed in 50 ml 10% formalin.Trawl Species: Banana prawns, herring/grinner/Australian threadfinThese species were processed onboard the trawl vessel according to processinginstructions outlined above (Step 1: Observations and data recorded by field staff at the timeof capture).Mud crabs Live crabs were held at 4°C for 15 minutes then transferred to -20°C until insensibleprior to necropsy Weight, carapace width and gender were recorded Photographs were taken of any shell lesions and internal organs Haemolymph was sampled for bacteriology. An aseptic sample of haemolymph wastaken from the heart using a syringe and sterile hypodermic needle through themembrane between thorax and abdomen (cleaned with alcohol first). This was thendischarged directly on marine agar with vitamin (MAV) plates for primary culture Gross evaluation with comment and scoring for:o Shell – lesions, noting ‘rust spot shell disease’ as outlined in the fieldobservations section above Gross evaluation with comment and scoring for:o Limbs – all present, one missing, more than one missingo Muscle – watery, translucent, opaque, contracted, multiple white spotso Gills – clean or fouled89
oooGonad – present/absentHepatopancreas – normal colouration or yellow/paleGut – normal/abnormalWeight of hepatopancreas recordedHistology - formalin fixing (1 cm x 1 cm in 50 ml 10% formalin):oooooolesionsgillhepatopancreasheartmusclegonad.Residue testing – Frozen: gill, hepatopancreas and claw muscle in separate containers eachcontaining at least 10 g (where possible).90
Page 1 and 2:
Department of Agriculture, Fisherie
Page 3 and 4:
ContentsTable of tables 1Table of f
Page 5 and 6:
Mullet (Mugilidae) 80Banana prawn (
Page 7 and 8:
Figure 7. Barramundi caught in the
Page 9 and 10:
Figure 38. Sharks caught in the Cal
Page 11 and 12:
Phase 1 (August 2011-February 2012)
Page 13 and 14:
The results in this study support t
Page 15 and 16:
ecognised expertise and research pu
Page 17 and 18:
Mr Mitchell reported high catches u
Page 19 and 20:
Figure 2. Sampling sites within the
Page 21 and 22:
Phase 2: Expanded Gladstone Fish He
Page 23 and 24:
Mud crab (Scylla serrata): Mud crab
Page 25 and 26:
For mud crabs, hepatopancreas, gill
Page 27 and 28:
Table 2. The number of fish, crusta
Page 29 and 30:
Significant findings and discussion
Page 31 and 32:
Commercial barramundi catch and cat
Page 33 and 34:
Figure 6. Eye condition observed in
Page 35 and 36:
Conditions observed in barramundi s
Page 37 and 38:
Figure 12. A barramundi caught in t
Page 39 and 40:
provided another environmental stre
Page 41 and 42:
was somewhat reduced because post-m
Page 43 and 44: 4,000Commercial effort (days fished
Page 45 and 46: Size structure of mud crabs observe
Page 47 and 48: Figure 21. Examples of shell abnorm
Page 49 and 50: Of the 58 crabs observed to have sh
Page 51 and 52: HistopathologyLipid storage vacuola
Page 53 and 54: Commercial fishing effort for mulle
Page 55 and 56: Figure 28. (a) Mullet caught in Rod
Page 57 and 58: June/July: Mullet samples were coll
Page 59 and 60: Sharks and raysThe monitoring progr
Page 61 and 62: Observational findings Phase 1Phase
Page 63 and 64: Encysted larval parasites were foun
Page 65 and 66: in particular bull sharks, have bee
Page 67 and 68: Commercial banana prawn catch for t
Page 69 and 70: Observational findings Phase 2April
Page 71 and 72: Laboratory testing Phase 1Seven riv
Page 73 and 74: four displayed signs of redness. Th
Page 75 and 76: Conditions observed in pelagic fish
Page 77 and 78: FinfishObservational findingsDuring
Page 79 and 80: ConclusionsIn 2011, a wide range of
Page 81 and 82: Mud crabsFisheries Queensland did n
Page 83 and 84: Owens, L. 1983. Bopyrid isopod Epip
Page 85 and 86: Candidate speciesThe monitoring pro
Page 87 and 88: Table 1: Candidate species for each
Page 89 and 90: Sample processingSample processing
Page 91 and 92: Herring/grinner/Australian threadfi
Page 93: For example: the third specimen, re
Page 97 and 98: Cloudy eye,swollen,redness orhemorr
Page 99 and 100: Red pin pointmarks - nogeneral redn
Page 101 and 102: Lesion photos Description GradeThis
Page 103 and 104: L4continuedoperculumgrazing;cuts or
Page 105 and 106: Appendix A-3: Fish health assessmen
Page 107 and 108: Appendix B - Statistical report: Gl
Page 109 and 110: analyses, but this was not done for
Page 111 and 112: was a significant (P < 0.05) locati
Page 113 and 114: Percentage with fin condition > 015
Page 115 and 116: Table 3.6b. Effects of locations an
Page 117 and 118: Table 3.7d. Spleen categories - cou
Page 119 and 120: 32Bundy2Bundy1UpBoyne1UpBoyne2LwBoy
Page 121 and 122: Table 3.9c. Regional group means an
Page 123 and 124: Table 4.2c. Effects of locations an
Page 125 and 126: 4.5 Summarising by regionsThe means
Page 127 and 128: Ta ble 5.2a. Effects of locations a
Page 129 and 130: 5.5 Summarising by regionsThe means
Page 131 and 132: 6.3 ResultsThere was no significant
Page 133 and 134: Table 6.5 lists the mean values whe
Page 135 and 136: Standardized residualsStandardized
Page 137 and 138: Table 2.3f. Muscle lesions.Lake Fit
Page 139 and 140: Table 4. 1f. Muscle lesions.Fitzroy
Page 141 and 142: Table 5.6. Regiona l m eans for the
Page 143 and 144: Contaminants in Tissue ofFish and C
Page 145 and 146:
Contaminants in Tissue of Fish and
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Department of Science, Information
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Appendix E - Time line BarraBy John
Page 205 and 206:
friends. Rapidly rising flood water
Page 207 and 208:
To make note - condition loss was e
Page 209 and 210:
May/June- FinsMy initial observatio
Page 211 and 212:
Thes e escapee were now also in awo
Page 213 and 214:
Figure N. Barramundi showing net ma
Page 215 and 216:
September- Deaths and SicknessFigur
Page 217 and 218:
There is numerous distinguishing fe
Page 219:
Department of Agriculture, Fisherie
show all

Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

Create successful ePaper yourself

Delete template?

Save as template?