Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

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Photo 3: Opening the abdomen of a herring to allow penetration of fixative to internal organs.Specimen identification numberWhere specimens had been selected for further processing, a specimen identificationnumber was assigned in the field. The number was written on waterproof paper andrecorded: Trip numbero e.g. T1, T2, T3 Site referenceo FIT – Fitzroyo NAR – Narrowso HAM – Hamilton Pointo CAL – Calliope Rivero SPG – Spoil groundso HTR – Harbour trawlo UPB – upper Boyne Rivero LOB – lower Boyne Rivero ROB – Rodds Bayo BUN – Bundaberg Specimen numbero 4 digits (e.g. 0001)87
For example: the third specimen, regardless of species collected from the upper Boyne onTrip 2, would be allocated a specimen number T2UPB0003. The specimen number wasrecorded on a waterproof label and remained with the specimen from capture to labprocessing.Step 2: Laboratory processingStep two involved the processing of samples that were kept for further analysis. Wherepossible, observations were quantified to allow proper comparison and analysis. All datawas recorded on a <strong>Fish</strong> <strong>Health</strong> Laboratory Datasheet by Biosecurity Queensland (Appendix2), and categories were used to develop fish health indices for crude overall comparison offish health (Appendix 3). Classification of necropsy criteria was based on Schmitt et al. 2000.Mullet, barramundi, queenfish and sharks Weight recorded Length recorded (forklength to nearest 10 mm) External photographs with close-up images captured of abnormalities Otoliths removed (for fisheries analysis, barramundi and mullet only) Analysis for skin ‘redness’ according to existing protocol (note this was done onboardwith live or freshly dead fish for post-mortem comparison) Lesion count and severity with samples fixed in formalin. If there was only one lesion,it was preserved for histology, taking a section of the lesion that spans theabnormality to normal skinEye condition, general condition, skin condition and abdomen were assessedaccording to the data sheet categoriesSwab taken from any skin lesionso A bacterial swab was inserted into a fresh scalpel cut edge of the skin lesionafter 1 ml of 70% ethanol was applied to disinfect the surface. The swabcollected blood or fluid from the wound and was replaced into its transportmedia, labelled and placed into a dry plastic zip-lock bag on ice orrefrigeration at 4 degrees celcius. These were sent to Biosecurity ScienceLaboratory in Coopers Plains for analysis within 72 hrsSkin sample taken – ensuring sample includes all layers of skin, epidermis throughdermis to start of muscleo Histolgy - ~2 cm sq in 50 ml 10% formalinGills observed for appearance, parasite load and sediment foulingo Sample taken for chemical residue testing - One side of gills, frozen. Thisconsisted of 2 aliquots each containing at least 10 g (where possible).o Sample for histology – 1 cm length of holobranch in 50 ml 10% formalin88
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Department of Agriculture, Fisherie
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ContentsTable of tables 1Table of f
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Mullet (Mugilidae) 80Banana prawn (
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Figure 7. Barramundi caught in the
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Figure 38. Sharks caught in the Cal
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Phase 1 (August 2011-February 2012)
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The results in this study support t
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ecognised expertise and research pu
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Mr Mitchell reported high catches u
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Figure 2. Sampling sites within the
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Phase 2: Expanded Gladstone Fish He
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Mud crab (Scylla serrata): Mud crab
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For mud crabs, hepatopancreas, gill
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Table 2. The number of fish, crusta
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Significant findings and discussion
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Commercial barramundi catch and cat
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Figure 6. Eye condition observed in
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Conditions observed in barramundi s
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Figure 12. A barramundi caught in t
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provided another environmental stre
Page 41 and 42: was somewhat reduced because post-m
Page 43 and 44: 4,000Commercial effort (days fished
Page 45 and 46: Size structure of mud crabs observe
Page 47 and 48: Figure 21. Examples of shell abnorm
Page 49 and 50: Of the 58 crabs observed to have sh
Page 51 and 52: HistopathologyLipid storage vacuola
Page 53 and 54: Commercial fishing effort for mulle
Page 55 and 56: Figure 28. (a) Mullet caught in Rod
Page 57 and 58: June/July: Mullet samples were coll
Page 59 and 60: Sharks and raysThe monitoring progr
Page 61 and 62: Observational findings Phase 1Phase
Page 63 and 64: Encysted larval parasites were foun
Page 65 and 66: in particular bull sharks, have bee
Page 67 and 68: Commercial banana prawn catch for t
Page 69 and 70: Observational findings Phase 2April
Page 71 and 72: Laboratory testing Phase 1Seven riv
Page 73 and 74: four displayed signs of redness. Th
Page 75 and 76: Conditions observed in pelagic fish
Page 77 and 78: FinfishObservational findingsDuring
Page 79 and 80: ConclusionsIn 2011, a wide range of
Page 81 and 82: Mud crabsFisheries Queensland did n
Page 83 and 84: Owens, L. 1983. Bopyrid isopod Epip
Page 85 and 86: Candidate speciesThe monitoring pro
Page 87 and 88: Table 1: Candidate species for each
Page 89 and 90: Sample processingSample processing
Page 91: Herring/grinner/Australian threadfi
Page 95 and 96: oooGonad - present/absentHepatopanc
Page 97 and 98: Cloudy eye,swollen,redness orhemorr
Page 99 and 100: Red pin pointmarks - nogeneral redn
Page 101 and 102: Lesion photos Description GradeThis
Page 103 and 104: L4continuedoperculumgrazing;cuts or
Page 105 and 106: Appendix A-3: Fish health assessmen
Page 107 and 108: Appendix B - Statistical report: Gl
Page 109 and 110: analyses, but this was not done for
Page 111 and 112: was a significant (P < 0.05) locati
Page 113 and 114: Percentage with fin condition > 015
Page 115 and 116: Table 3.6b. Effects of locations an
Page 117 and 118: Table 3.7d. Spleen categories - cou
Page 119 and 120: 32Bundy2Bundy1UpBoyne1UpBoyne2LwBoy
Page 121 and 122: Table 3.9c. Regional group means an
Page 123 and 124: Table 4.2c. Effects of locations an
Page 125 and 126: 4.5 Summarising by regionsThe means
Page 127 and 128: Ta ble 5.2a. Effects of locations a
Page 129 and 130: 5.5 Summarising by regionsThe means
Page 131 and 132: 6.3 ResultsThere was no significant
Page 133 and 134: Table 6.5 lists the mean values whe
Page 135 and 136: Standardized residualsStandardized
Page 137 and 138: Table 2.3f. Muscle lesions.Lake Fit
Page 139 and 140: Table 4. 1f. Muscle lesions.Fitzroy
Page 141 and 142: Table 5.6. Regiona l m eans for the
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Contaminants in Tissue ofFish and C
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Contaminants in Tissue of Fish and
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Department of Science, Information
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Appendix E - Time line BarraBy John
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friends. Rapidly rising flood water
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To make note - condition loss was e
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May/June- FinsMy initial observatio
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Thes e escapee were now also in awo
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Figure N. Barramundi showing net ma
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September- Deaths and SicknessFigur
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There is numerous distinguishing fe
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Department of Agriculture, Fisherie
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Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

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