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Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

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o lesions present/absent (estimated % shell affected)o fouling of the shell present/absentAll specimens selected for further testing were allocated a specimen number andphotographed (specimen identification number label visible in all images).Live crabs were submitted for necropsy examination by Biosecurity Queensland.Banana prawns Carapace length (notch at base of rostrum to posterior margin of carapace) Shell lesions or erosion present/absent Visible parasites present/absent All specimens selected for further testing were allocated a specimen identificationnumber and photographed on both sides with close-up images captured ofabnormalities (specimen identification number label visible in all images). Thesespecimens were processed immediately after capture onboard the trawler by aveterinarian with aquatic animal health expertise.o Shell condition was rated and any lesions or irregularities photographed.Swabs were taken for bacteriology testing of any major lesions.o Cephalothorax/ hepatopancreas was injected with 1-2 ml 10% formalin.Prawns were cut through with a scalpel immediately posterior to thecephalothorax. Shell covering gills was removed on one side, heads fixedwhole in 50 ml 10% formalin fixative (see photo 1).o Tails were frozen for residue testing in separate containers.Photo 1: Injecting the cephalothorax of a banana prawn with 10% formalin, and removing the gill cover tooptimize fixation of tissues.85

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