Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...
Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ... Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...
SamplingField observationsIn the field up to 100 individuals per site per trip, of each candidate species were observed.Their lengths and external health condition were recorded on field data sheets at the time ofcapture. From the observed individuals, 10 were retained for further processing. Theremaining individuals were returned to the water alive with the exception of some mud crabswhich were retained by the commercial fisher.Samples selected for further processingTen individuals per site per trip, of each candidate species (targeting five apparently healthyand five apparently unhealthy) were selected for further testing by Biosecurity Queensland.Fish/sharks were selected as unhealthy based on the presence of: lesions and skin discolouration as per Appendix 1 eye lesions as per Appendix 1 swelling or unusual mass on the abdomen signs of emaciation (i.e. large head, thin body, poor body condition) haemorrhage from gills or vent.Crabs were selected as unhealthy based on the presence of: rust spots shell erosion fouling of the shell lost appendages.Prawns were selected as unhealthy based on the presence of: presence of lesions/shell erosion parasitic infestation.Whilst 10 might seem a relatively low number, the overall level of replication is built upacross sites and times. Hence, the expectation of 10 fish/crabs/sharks/prawns per sampleper site per trip, should ultimately give adequate coverage across sexes, ages, lengths, andany other influential factors, so that these effects can then be taken into account in theoverall analysis of data.83
Sample processingSample processing involved two steps:Step 1: Observations and data recorded by field staff at the time of capture.For each species, the following data was recorded in the field on a Fish Health FieldDatasheet: site details time of capture specimen identification number on waterproof label where appropriate (see below).Mullet, barramundi, queenfish and sharks Length (forklength) was measured to the nearest 10 mm. Skin and eye condition was classified according to Appendix 1. All specimens selected for necropsy examination were allocated a specimenidentification number, photographed on both sides with close-up images captured ofabnormalities (specimen identification number label visible in all images), euthanized(blunt force trauma), individually bagged and transported on ice to the laboratory.Mud crabs Carapace width (notch to notch) was recorded to the nearest mm using calipers. Sex recorded An assessment of:o limbs missingo ‘rust’ spots number and grade as per criteria outlined in Table A-2Table A-2: Grading system used to classify shell lesions as described by Andersen (2003).GradeDescriptionGrade 1Non perforated < 5 mm diameterGrade 2Non perforated > or = 5 mm diameterGrade 3 Perforated cuticle (either partially or fully) or = 5mm diameter and < 20 mm diameterGrade 5 Perforated cuticle (either partially or fully) >or = 20 mm diameter84
- Page 37 and 38: Figure 12. A barramundi caught in t
- Page 39 and 40: provided another environmental stre
- Page 41 and 42: was somewhat reduced because post-m
- Page 43 and 44: 4,000Commercial effort (days fished
- Page 45 and 46: Size structure of mud crabs observe
- Page 47 and 48: Figure 21. Examples of shell abnorm
- Page 49 and 50: Of the 58 crabs observed to have sh
- Page 51 and 52: HistopathologyLipid storage vacuola
- Page 53 and 54: Commercial fishing effort for mulle
- Page 55 and 56: Figure 28. (a) Mullet caught in Rod
- Page 57 and 58: June/July: Mullet samples were coll
- Page 59 and 60: Sharks and raysThe monitoring progr
- Page 61 and 62: Observational findings Phase 1Phase
- Page 63 and 64: Encysted larval parasites were foun
- Page 65 and 66: in particular bull sharks, have bee
- Page 67 and 68: Commercial banana prawn catch for t
- Page 69 and 70: Observational findings Phase 2April
- Page 71 and 72: Laboratory testing Phase 1Seven riv
- Page 73 and 74: four displayed signs of redness. Th
- Page 75 and 76: Conditions observed in pelagic fish
- Page 77 and 78: FinfishObservational findingsDuring
- Page 79 and 80: ConclusionsIn 2011, a wide range of
- Page 81 and 82: Mud crabsFisheries Queensland did n
- Page 83 and 84: Owens, L. 1983. Bopyrid isopod Epip
- Page 85 and 86: Candidate speciesThe monitoring pro
- Page 87: Table 1: Candidate species for each
- Page 91 and 92: Herring/grinner/Australian threadfi
- Page 93 and 94: For example: the third specimen, re
- Page 95 and 96: oooGonad - present/absentHepatopanc
- Page 97 and 98: Cloudy eye,swollen,redness orhemorr
- Page 99 and 100: Red pin pointmarks - nogeneral redn
- Page 101 and 102: Lesion photos Description GradeThis
- Page 103 and 104: L4continuedoperculumgrazing;cuts or
- Page 105 and 106: Appendix A-3: Fish health assessmen
- Page 107 and 108: Appendix B - Statistical report: Gl
- Page 109 and 110: analyses, but this was not done for
- Page 111 and 112: was a significant (P < 0.05) locati
- Page 113 and 114: Percentage with fin condition > 015
- Page 115 and 116: Table 3.6b. Effects of locations an
- Page 117 and 118: Table 3.7d. Spleen categories - cou
- Page 119 and 120: 32Bundy2Bundy1UpBoyne1UpBoyne2LwBoy
- Page 121 and 122: Table 3.9c. Regional group means an
- Page 123 and 124: Table 4.2c. Effects of locations an
- Page 125 and 126: 4.5 Summarising by regionsThe means
- Page 127 and 128: Ta ble 5.2a. Effects of locations a
- Page 129 and 130: 5.5 Summarising by regionsThe means
- Page 131 and 132: 6.3 ResultsThere was no significant
- Page 133 and 134: Table 6.5 lists the mean values whe
- Page 135 and 136: Standardized residualsStandardized
- Page 137 and 138: Table 2.3f. Muscle lesions.Lake Fit
Sample processingSample processing involved two steps:Step 1: Observations and data recorded by field staff at the time of capture.For each species, the following data was recorded in the field on a <strong>Fish</strong> <strong>Health</strong> FieldDatasheet: site details time of capture specimen identification number on waterproof label where appropriate (see below).Mullet, barramundi, queenfish and sharks Length (forklength) was measured to the nearest 10 mm. Skin and eye condition was classified according to Appendix 1. All specimens selected for necropsy examination were allocated a specimenidentification number, photographed on both sides with close-up images captured ofabnormalities (specimen identification number label visible in all images), euthanized(blunt force trauma), individually bagged and transported on ice to the laboratory.Mud crabs Carapace width (notch to notch) was recorded to the nearest mm using calipers. Sex recorded An assessment of:o limbs missingo ‘rust’ spots number and grade as per criteria outlined in Table A-2Table A-2: Grading system used to classify shell lesions as described by Andersen (2003).GradeDescriptionGrade 1Non perforated < 5 mm diameterGrade 2Non perforated > or = 5 mm diameterGrade 3 Perforated cuticle (either partially or fully) or = 5mm diameter and < 20 mm diameterGrade 5 Perforated cuticle (either partially or fully) >or = 20 mm diameter84