Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

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Contaminants in Tissue of Fish and Crabs Collected in the Gladstone AreaTable 2.4. Regional means for the metal concentrations (dry-weight basis). Within trips, significant(P < 0.05) differences are bolded.Trip 1 – Trip 2 –Reference Gladstone Sig. level Reference Gladstone Sig. levelAluminium 16.7 14.7 0.646 12.2 9.0 0.513Chromium 0.186 0.098 0.031 0.120 0.166 0.288Iron 273 188 0.057 187 418 0.000Nickel 3.22 3.16 0.938 1.48 2.43 0.306Copper 526 1127 0.004 621 294 0.139Zinc 103 195 0.002 209 242 0.275Arsenic 17.3 20.2 0.419 23.2 27.4 0.285Selenium 5.89 7.46 0.027 5.03 7.36 0.004Silver 4.80 3.67 0.401 4.91 3.18 0.251Cadmium 2.47 1.55 0.268 4.45 2.41 0.030Barium 1.55 0.68 0.021 2.25 0.97 0.003Mercury 0.283 0.245 0.349 0.416 0.155 0.000Lead 0.155 0.069 0.046 0.072 0.180 0.024Again, the means for lead are based on relatively few actual values, so should be considered withsome caution.ReferencesGenStat (2011). GenStat for Windows, Release 14.1. VSN International Ltd., Oxford.McCullagh, P. and Nelder, J. A. (1989). Generalized Linear Models (2 nd ed.). Chapman and Hall,London.Raj, D. (1968). Sampling theory. McGraw-Hill, New York.Page 39
Department of Science, Information Technology, Innovation and the ArtsAppendix BStatistical Report – Metals, Barramundi and Grinner1. STATISTICAL METHODSGeneral linear models (McCullagh and Nelder 1989) were used for the analyses of the metalconcentrations in fish (on a dry-weight basis), using GenStat (2011). These were done separately forthe gill and liver samples of the barramundi, and also for the muscle samples from the grinner. Eachfish was taken as an independent experimental unit. For samples reported as ‘below the detectionlimit’, the usual convention of substituting half the detection level was used. This was done for thewet-weight data (upon which the test had been performed), and then these values were corrected formoisture per cent back to a dry-weight basis (which were used for the analyses).Again, the deliberate sampling of both diseased and unaffected barramundi introduced furthercomplexity into the analyses. To adjust for this stratification, weighting factors were calculated as –Proportion (normal or diseased) in the whole sample /Proportion (normal or diseased) in the laboratory subsampleThese weighting factors were determined at the location by trip level, and then used to correctlyweight each of the respective observations (normal or diseased) in the analyses for trips bylocations. As none of the grinner were rated as diseased, no weightings were adopted for theiranalyses.For some metals, most of the observations were classified as LOR) observations. For these no valid analysis is possible – evennonparametric tests show no result when there are only a few (possibly-random) actual values. Forexample, the ‘most extreme’ regional comparison in Table 1.1 is two positives for Gladstone vs.zero for the reference sites, and this Chi-square test gives P = 0.15. For the metals which hadsufficient data to be assumed as continuous there were a few high values, however these data werenot notably skewed so no transformations were required.Table 1.1a. For the barramundi gill samples, those metals with few observations >LOR, listingtheir counts across sites and trips (blanks = zero).Chromium Nickel Silver LeadTRIP 1 - Awoonga DamUpper Boyne River 1Hamilton PointBundabergFitzroy RiverTRIP 2 - Awoonga DamUpper Boyne River 1Hamilton Point 1 1 1Bundaberg 1Fitzroy RiverPage 40
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Department of Agriculture, Fisherie
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ContentsTable of tables 1Table of f
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Mullet (Mugilidae) 80Banana prawn (
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Figure 7. Barramundi caught in the
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Figure 38. Sharks caught in the Cal
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Phase 1 (August 2011-February 2012)
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The results in this study support t
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ecognised expertise and research pu
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Mr Mitchell reported high catches u
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Figure 2. Sampling sites within the
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Phase 2: Expanded Gladstone Fish He
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Mud crab (Scylla serrata): Mud crab
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For mud crabs, hepatopancreas, gill
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Table 2. The number of fish, crusta
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Significant findings and discussion
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Commercial barramundi catch and cat
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Figure 6. Eye condition observed in
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Conditions observed in barramundi s
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Figure 12. A barramundi caught in t
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provided another environmental stre
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was somewhat reduced because post-m
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4,000Commercial effort (days fished
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Size structure of mud crabs observe
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Figure 21. Examples of shell abnorm
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Of the 58 crabs observed to have sh
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HistopathologyLipid storage vacuola
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Commercial fishing effort for mulle
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Figure 28. (a) Mullet caught in Rod
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June/July: Mullet samples were coll
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Sharks and raysThe monitoring progr
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Observational findings Phase 1Phase
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Encysted larval parasites were foun
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in particular bull sharks, have bee
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Commercial banana prawn catch for t
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Observational findings Phase 2April
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Laboratory testing Phase 1Seven riv
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four displayed signs of redness. Th
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Conditions observed in pelagic fish
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FinfishObservational findingsDuring
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ConclusionsIn 2011, a wide range of
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Mud crabsFisheries Queensland did n
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Owens, L. 1983. Bopyrid isopod Epip
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Candidate speciesThe monitoring pro
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Table 1: Candidate species for each
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Sample processingSample processing
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Herring/grinner/Australian threadfi
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For example: the third specimen, re
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oooGonad - present/absentHepatopanc
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Cloudy eye,swollen,redness orhemorr
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Red pin pointmarks - nogeneral redn
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Lesion photos Description GradeThis
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L4continuedoperculumgrazing;cuts or
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Appendix A-3: Fish health assessmen
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Appendix B - Statistical report: Gl
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analyses, but this was not done for
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was a significant (P < 0.05) locati
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Percentage with fin condition > 015
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Table 3.6b. Effects of locations an
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Table 3.7d. Spleen categories - cou
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32Bundy2Bundy1UpBoyne1UpBoyne2LwBoy
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Table 3.9c. Regional group means an
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Table 4.2c. Effects of locations an
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4.5 Summarising by regionsThe means
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Ta ble 5.2a. Effects of locations a
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5.5 Summarising by regionsThe means
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6.3 ResultsThere was no significant
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Table 6.5 lists the mean values whe
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Standardized residualsStandardized
Page 137 and 138: Table 2.3f. Muscle lesions.Lake Fit
Page 139 and 140: Table 4. 1f. Muscle lesions.Fitzroy
Page 141 and 142: Table 5.6. Regiona l m eans for the
Page 143 and 144: Contaminants in Tissue ofFish and C
Page 145 and 146: Contaminants in Tissue of Fish and
Page 151 and 152: Department of Science, Information
Page 187: Department of Science, Information
Page 203 and 204: Appendix E - Time line BarraBy John
Page 205 and 206: friends. Rapidly rising flood water
Page 207 and 208: To make note - condition loss was e
Page 209 and 210: May/June- FinsMy initial observatio
Page 211 and 212: Thes e escapee were now also in awo
Page 213 and 214: Figure N. Barramundi showing net ma
Page 215 and 216: September- Deaths and SicknessFigur
Page 217 and 218: There is numerous distinguishing fe
Page 219: Department of Agriculture, Fisherie
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