Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

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Contaminants in Tissue of Fish and Crabs Collected in the Gladstone AreaEHP (Department of Environment and Heritage Protection). 2012i. September 2012 Test of WaterQuality in Port Curtis. Available from .Gladstone Fish Health Scientific Advisory Panel 2012. Gladstone Fish Health Scientific AdvisoryPanel – Final Report 5 January 2012. 47p. Available from.Hamilton SJ, Mehrle PM, and Jones JR. (1987) Cadmium-saturation technique for measuringmetallothionein in brook trout: Transactions of American Fisheries Society 116:541-550.Mortimer MR. (2000) Pesticide and trace metal concentrations in Queensland estuarine crabs.Marine Pollution Bulletin 41: 359-366.Negri AP, Mortimer M, Carter S and Mueller JF. (2009) Persistent organochlorines and metals inestuarine mud crabs of the Great Barrier Reef. Marine Pollution Bulletin. 58: 765-786.Poulsen AH and Escher BI 2012. Chemically induced immunosuppression and diseasesusceptibility in marine wildlife: A literature review. National Research Centre for environmentalToxicology (Entox), The University of Queensland, Brisbane, Queensland. 110p.Stevens J. (1986). Applied multivariate statistics for the social sciences. Hillsdale, NJ: LawrenceErlbaum Associates.US Army Corp and USEPA. 2011. The Environmental Residue and Effects Database. Availablefrom: http://el.erdc.usace.army.mil/ered/. Last updated October 2011. Downloaded: 12 April 2013.Page 33
Department of Science, Information Technology, Innovation and the ArtsAppendix AStatistical Report – Metals, Crabs1. STATISTICAL METHODSGeneral linear models (McCullagh and Nelder 1989) were used for the analyses of the metalconcentrations (on a dry-weight basis), under GenStat (2011). There were 40 crabs in total - 5samples (containing both normal and abnormal crabs) from each combination of the four locationsby two trips. Each crab was taken as an independent experimental unit. For samples reported as‘below the detection limit’, the usual convention of substituting half the detection level was used.This was done for the wet-weight data (upon which the test had been performed), and then thesevalues were corrected for moisture per cent back to a dry-weight basis (which were used for theanalyses).Again, the deliberate sampling of both diseased and unaffected crabs introduced further complexityinto the analyses. To adjust for this stratification, weighting factors were calculated as –Proportion (normal or abnormal) in the whole sample /Proportion (normal or abnormal) in the laboratory subsampleThese weighting factors were determined at the location by trip level, and then used to correctlyweight each of the respective observations (normal or abnormal) in the analyses for trips bylocations.Crab weight was trailed as a covariate, but for the 13 metals analysed was only significant (P =0.038) for barium, and this fitted as a negative. There were no other ‘near-significant’ fits; nor werethe fitted slopes predominantly positive (six out of 13 were negative). Similarly, carapace width hadno association with increased metal levels over time, with none being significant and seven fittingas negative slopes. Hence the expected bio-accumulation effect could not be estimated and adjustedfor, and so was dropped for all analyses.In addition to conducting the primary weighted analyses of location by trip (along with theinteraction), second analyses were conducted for each variable including ‘disease status’ in themodel. This allowed the direct testing and reporting of the impact of disease status on eachconcentration.The univariate analyses of each of the metals are followed by a multivariate summarisation. Thisfirst considers the relationships amongst the metals (via a correlation matrix), and then adopts aprincipal components representation of all the data (taking the first two orthogonal dimensions) toobtain an overview as to which locations and times are most similar.2. RESULTSWhilst there were a few high values for some of the metals, in general these data were not skewed,so no transformations were required. Of the 13 analyses, significant differences were found betweenlocations in seven and between trips in five. Importantly, there was a significant (P < 0.05) locationby trip interaction in four of the 13 analyses, with this effect being near-significant (0.05 < P
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Department of Agriculture, Fisherie
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ContentsTable of tables 1Table of f
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Mullet (Mugilidae) 80Banana prawn (
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Figure 7. Barramundi caught in the
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Figure 38. Sharks caught in the Cal
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Phase 1 (August 2011-February 2012)
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The results in this study support t
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ecognised expertise and research pu
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Mr Mitchell reported high catches u
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Figure 2. Sampling sites within the
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Phase 2: Expanded Gladstone Fish He
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Mud crab (Scylla serrata): Mud crab
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For mud crabs, hepatopancreas, gill
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Table 2. The number of fish, crusta
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Significant findings and discussion
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Commercial barramundi catch and cat
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Figure 6. Eye condition observed in
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Conditions observed in barramundi s
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Figure 12. A barramundi caught in t
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provided another environmental stre
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was somewhat reduced because post-m
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4,000Commercial effort (days fished
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Size structure of mud crabs observe
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Figure 21. Examples of shell abnorm
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Of the 58 crabs observed to have sh
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HistopathologyLipid storage vacuola
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Commercial fishing effort for mulle
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Figure 28. (a) Mullet caught in Rod
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June/July: Mullet samples were coll
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Sharks and raysThe monitoring progr
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Observational findings Phase 1Phase
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Encysted larval parasites were foun
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in particular bull sharks, have bee
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Commercial banana prawn catch for t
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Observational findings Phase 2April
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Laboratory testing Phase 1Seven riv
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four displayed signs of redness. Th
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Conditions observed in pelagic fish
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FinfishObservational findingsDuring
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ConclusionsIn 2011, a wide range of
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Mud crabsFisheries Queensland did n
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Owens, L. 1983. Bopyrid isopod Epip
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Candidate speciesThe monitoring pro
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Table 1: Candidate species for each
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Sample processingSample processing
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Herring/grinner/Australian threadfi
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For example: the third specimen, re
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oooGonad - present/absentHepatopanc
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Cloudy eye,swollen,redness orhemorr
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Red pin pointmarks - nogeneral redn
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Lesion photos Description GradeThis
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L4continuedoperculumgrazing;cuts or
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Appendix A-3: Fish health assessmen
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Appendix B - Statistical report: Gl
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analyses, but this was not done for
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was a significant (P < 0.05) locati
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Percentage with fin condition > 015
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Table 3.6b. Effects of locations an
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Table 3.7d. Spleen categories - cou
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32Bundy2Bundy1UpBoyne1UpBoyne2LwBoy
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Table 3.9c. Regional group means an
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Table 4.2c. Effects of locations an
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4.5 Summarising by regionsThe means
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Ta ble 5.2a. Effects of locations a
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5.5 Summarising by regionsThe means
Page 131 and 132: 6.3 ResultsThere was no significant
Page 133 and 134: Table 6.5 lists the mean values whe
Page 135 and 136: Standardized residualsStandardized
Page 137 and 138: Table 2.3f. Muscle lesions.Lake Fit
Page 139 and 140: Table 4. 1f. Muscle lesions.Fitzroy
Page 141 and 142: Table 5.6. Regiona l m eans for the
Page 143 and 144: Contaminants in Tissue ofFish and C
Page 145 and 146: Contaminants in Tissue of Fish and
Page 151 and 152: Department of Science, Information
Page 181: Department of Science, Information
Page 203 and 204: Appendix E - Time line BarraBy John
Page 205 and 206: friends. Rapidly rising flood water
Page 207 and 208: To make note - condition loss was e
Page 209 and 210: May/June- FinsMy initial observatio
Page 211 and 212: Thes e escapee were now also in awo
Page 213 and 214: Figure N. Barramundi showing net ma
Page 215 and 216: September- Deaths and SicknessFigur
Page 217 and 218: There is numerous distinguishing fe
Page 219: Department of Agriculture, Fisherie
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Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

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