Gladstone Fish Health Investigation 2011 - 2012 - Western Basin ...

Table 4. 1f. Muscle lesions.Fitzroy River BundabergGladstone Harbour A vg.s.e.Trip 1 0.00 0.20 0.10Trip 2 0.00 0.00 0.00For grinners, there was no significant effect of location (P = 0.58) or trip (P = 0.64) on the classification ofliver lipid. Table 4.2 shows that the counts by locations are well distributed across the liver lipidc ategories.Table 4.2. Counts for liver lipid categories b y locations.Fitzroy River Bund aberg Gladstone HarbourAbsent 3 2 2Mild 4 3 4Moderate 2 0 4Marked 1 0 05. RESULTS – MUD CRABSGill hyperplasia and gill parasites were measured for all 40 mud crabs. However, the data forhepatopancreas, hepatopancreas lipid, heart, cuticle lesions and muscle lesions all contained missingobservations. This is unfortunate, as the cumulative score can only be calculated for crabs which have allscores measured, and there are only 19 observations here.Carapace width, gender and crab category were all trialed as covariates. From 21 analyses only two weresignificant (P < 0.05), approximately matching the random expectation of 1.05, so no covariate was usedfor the final analyses. Again, the distributions of the residuals justified the statistical assumption ofuntransformed continuous data under a Normal distribution.Disease status – As listed in Table 5.1, only cuticle lesions showed a significant (P < 0.05) differencebetween abnormal and normal crabs.Table 5.1. Effects of disease status on the histologyratings for m ud crabs.Abnormal NormalAverages.e. Sig. level (P)Cumulative score 2.33 1.95 0.63 0.67Gill hyperplasia 0.64 0.70 0.16 0.77Gill parasites 0.78 0.68 0.15 0.66Hepatopancreas 0.13 0.00 0.08 0.27Heart 0.13 0.13 0.11 1.00Cuticle lesions 0.41 0.00 0.11 0.01Muscle lesions 0.27 0.25 0.17 0.92There was no significant effect (P = 0.40) of disease rating on the classification of hepatopancreas lipid.Table 5.2 shows that the counts are well distributed across the hepatopancreas lipid categories.Table 5.2. Counts for he patopancreas lipidcategories by disease status.AbsentM ild ModerateAbnormal 2 9 4Normal 1 9 9Location by trip meansThese results are listed in Tables 5.3a to 5.3g. Again, significant‐difference testing has only been indicatedon the four tables that had some proven differences amongst the means.Table 5. 3a. Cumulativescore.Bundaberg Fitzroy RiverThe NarrowsPort AreaAvg . s.e.Trip 1 3.43 a 2.94 a 0.81 b 2.00 ab 1.29Trip 2 3.00 a 0.11 b 0.75 b 3.00 aa Means with a common superscript are not significantly different (P = 0.05).134