PNABD246.pdf

*0309 Provvidenti, R. 1986. Seed transmission of blackeye cowpea mosaic
virus in Vigna mungo. PLANT DISEASE, v.70(1O):981. [En] [En Abst] (A:PS)
During greenhouse evaluations of leguminous species for disease resistance,
viral symptoms were noted on seedlings of an accession of blackgram (Vigna
mungo (L.) flepper). Seeds of PI 222823 were collected at Bukan, Western
Azerbaijan (Iran) in 1954 and erroneously listed as mungbean (V. radiata (L.)
Wilczek). The original collection was sent to the USDA Northeast Regional Plant
Introduction Station, Geneva, NY, from which we obtained a sample years later.
Symptoms consisted of a diffuse green mottle, foliar cupping along the main
vein, and plant stunting. The causal agent was identified as blackeye cowpea
mosaic virus (BICMV) by electron microscopy, serology, host i.nge, and absence
of infection in the cowpcq line TVU 2480, which possesses a specific gene for
resistance to BICMV. In the original seed lot, the rate of virus transmission
was 14Z. However, a higher proportion of BICMV-infected plants was detected in
progenies of individual plants, ranging from 20 to 48%. Before planting, seed
lots had been kept in storage for 3 yr. In BICMV-infected seed, the virus was
recovered from embryos but not from testae. This is believed to be the first
report of seed transmission of BICMV in V. mungo. [AS]
*0310 Mink, G.I. 1987. First report of cowpea mild mottle virus on bean and
mung bean in Tanzania [Abst.]. PLANT DISEASE, v.71(6):5571. [En Abstj (A:PS)
Using indirect enzyme-linked immunosorbent assay and an antiserum, cowpea
mild mottle virus (CMMV), a carlavirus, was detected in leaf tissue from three
bean (Phaseolus vulgaris L.) and two mungbean (Vigna radiata) plants collected
on the Sokoine University research farm located near Morogoro, Tanzania.
Typical carlavirus particles with modal lengths of 600-650 nm were observed in
leaf-dip preparations from infected bean and mungbean tissues. Mild but
distincr veinclearing and mottle were observed on both infected mungbean
plants. No symptom attributable to CMMV was found on the three infected bean
plants. CMMV was not detected in over 100 bean leaf samples tested from private
farms and research stations in other areas of Tanzania and Kenya. Occurrence of
CMMV only in and near plots of imported germplasm suggested that the virus was
introduced into the area through infected mungbean seed lots from India and
subsequently spread to a few nearby bean plants. No residual mungbean seed was
available. [AS/THH]
MYCOPLASMA-LIKE DISEASES
*0311 Singh, D. 1954. Phyllody in mung (Phaseolus aureus L.). SCIENCE AND
CULTURE, v.20(4):194. [En] (REP.MB-1374)
The mycoplasma-like disease was first noted in a summer crop of mungbean in
1952, and again noted in kharif season of 1952 and 1953. The plants are
characterized by the transformation of the flower parts into leaf-like
structures. [THH]
*0312 Iwaki, M. ; Roechan, M. ; Saleh, N. ; Sugiura, M. ; Hibino, H. 1978.
Identity of Mycoplasma-like agents of legume witches' brooms in Indonesia.
CONTRIBUTIONS CRIA, no.41:i-11. [EnJ [En Abstj (REP.MB-2754)
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