PNABD246.pdf

apparently did not transmit the virus. Identification of AMV strains isolated
from naturally infected food and forage legumes was based on symptomatology,
host range, host reactions, serology and vector transmission mode. [ASJ
*0501 Lazar, E. ; Walter, E. ; Stussi-Garaud, C. ; Hirth, L. 1979.
RNA-dependent RNA polymerases from healthy and tobacco necrosis virus-infected
Phaseolus aureus: assay of localization in fractions of cellular homogenates.
VIROLOGY, v.96(2):553-563. tEnJ [En Abst] (REP.MB-2154)
"Sedimenting" and "sol jble" RNA-dependent RNA polymerases were detected, in
healthy and tobacco necrosis virus (TNV)-infected cotyledons of Vigna radiata,
in the 30,000 g pellet and the 100,000 g supernatant, respectively. The
activity recovered in the 30,000 g pellet is commonly assumed to be a
"membraue-bound" enzyme. Attempts to localize the "bound" RNA-dependent RNA
polymerases of both healthy and infected cotyledons on a defined membrane
fraction were made after linear sucrose gradient centrifugation of the 1000 g
supernatants containing the membranes. Each membrane fraction was localized in
the gradient by assaying for characteristic enzymes known to be associated with
it. RNA-dependent RNA polymerase activities of the 1000 g supernatants from
both healthy and TNV-infected extracts migrated in the gradient to a position
of 22-24% sucrose. This fraction of the gradient contained an activity
sedimenting at 100,000 g together with nucleoproteic structures and also a
soluble activity. The soluble RNA-dependent RNA polymerases contained in the
10.;,000 g supernatant migrated to the same depth in the gradient. These
activities are not associated with any of the types of membranes characterized
and thus the problem of the existence of an actual "bound" RNA-dependent RNA
polymerase must be reconsidered. IASJ
*0302 Singh, A.K. ; Siagh, Raj Bahadur 1980. Alterations in protein yield
and protein qualtiy in utd bean infected by southern bean mosaic virus.
ANNALES DE PHYTOPATHOLOGIE, v12(2):131-138. [EnJ [En Abstj (REP.MB-2247)
The effect of southern bean mosaic virus (SBMV) on urd bean (Vigna mungo
(L.) Hepper) was studied. Virus infection increased the total N, protein N, ash
value in leaf and leaf protein concentrates (LPC). Extractability of LPC, p.
100 extractability of total N and protein N were higher and dry matter was
lower in diseased leaf than healthy one. Virus infection also increased the
fiber, lipid and phosphorus but decreased the total soluble sugars, starch and
calorific value in diseased samples. Free amino acids were also found higher in
diseased samples but enzymatic hydrolysis (in vitro digestibility) was lowered
in the infected plants. [AS/THHJ
*0303 Singh, B.R. ; Singh, Raj Bahadur ; Singh, H.C. ; Gangulee, R. 1980.
Observation on a virus disease of coffee senna. INDIAN JOURNAL OF MYCOLOGY
AND PLANT PATHOLOGY, v.9(1):101. [Enj (EP.MB-2608)
This note describes a virus disease of Cassia occidentalis. The virus
caused mosaic symptoms on Vigna mungo and V. sinensis. A comparison of host
range, physical properties and mode of transmission suggests that the present
virus differs from all the viruses previously reported on Cassia occidentalis.
[THH]
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