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virus by the host range, symptoms, transmissibility by aphids, stability in<br />

crude juice, morphology, inclusions and serological relationship. The virus was<br />

transmitted easily by juice inoculation and also by aphids, but not through<br />

seed of soybean and mungbean. Eighteen plant species of 5 families out of 34<br />

plant species of 11 families were infected by juice inoculation. The virus<br />

withstood heating at 55 C for 10 min but not 60 C, dilution of 1.UE-3 but not<br />

of 1.0E-", ageing of 7 days but not 14 days at room temperature (22-30 C). The<br />

virus particles were elongated flexuous rods, 760 nm in length. Inclusions<br />

induced by infection of the virus were similar to those produced by bean yellow<br />

mosaic virus group. The virus showed positive reaction to antiserum against<br />

bean yellow mosaic virus. [AS]<br />

*0298 Benigno, D.R.A. 1979. An aphid-borne virus isolated from blackgrom in<br />

the Philippines. PHILIPPINE AGRICULTURIST, v.62(4):328-332. [En] [En Abst]<br />

(A:PS)<br />

A flexuous rod virus, similar in properties to the bean common mosaic<br />

virus, was isolated from blackgram (Vigna mungo). The virus was mechanically<br />

transmitted by 3 species of aphids (Aphis craccivora Koch., A. gossypii Glover<br />

and Rhopalosiphum maidis Fitch). Tests on the stability of the virus in crude<br />

extract revealed that its thermal inactivation point was between b0 and 70 C,<br />

the dilution end point between I.OE-3 and 1.OE-4, and its longevity in vitro<br />

was 3 days. It systemically infected blackgram, mungbean, and cowpea while<br />

Chenopodium amaranticolor and C. quinoa reacted by chlorotic local lesions,<br />

often followed by systemic vein yellowing. No infections were observed in<br />

Datura stramonium, Gomphrena globosa, Nicotiana glutinosa, N. rustica, N.<br />

tabacum, Vicia faba 'Xanthi' and Zinnia elegans. [AS]<br />

*0299 Farrag, S.H. ; Kandaswamy, T.K. 1979. Soybean and greengram as local<br />

lesion assay hosts for sugarcane mosaic virus. CURRENT SCIENCE, v.48(19):865.<br />

[En] (REP.MB-2186)<br />

Soybean and mungbean were found to be local lesion hosts for sugarcane<br />

mosaic virus. The local lesions could be seen on the second trifoliate leaves<br />

within two days after inoculation in mungbean and five days in soybeans. The<br />

lesions in mungbean were irregular and purple, while in soybean they were<br />

well-defined and necrotic. [EMS]<br />

*0300 Kaiser, W.J. 1979. Natural infection of cowpea and mung bean by<br />

alfalfa mosaic virus in Iran. PLANT DISEASE REPORTER, v.63(5):414-418. [En]<br />

[En Abst] (REP.MB-2086)<br />

Field plantings of cowpea (Vigna unguiculata) and mungbean (Vigna radiata)<br />

in Iran were naturally infected by alfalfa mosaic virus (AMV) during 1966-1971.<br />

Cowpea and mungbean plants typically were stunted and their foliage exhibited<br />

yellow mosaic (calico) symptoms, as did the pods of some infected cowpea<br />

accessions. The incidence of AMV in cowpea and mungbean plantings was usually<br />

less than 10%. Inoculation of cowpea in the field with AMV strains from cowpea<br />

or alfalfa at the full bloom and prebloom stages reduced seed yields about 15%<br />

and 50%, respectively. No mortality of infected plants was observed in these<br />

studies. The virus was transmitted by the pea aphid (Acyrthosiphon pisum) in a<br />

stylet-borne (noncirculative) manner from AMV-infected alfalfa and cowpea to<br />

healthy cowpea. Seeds harvested from naturally or artificially infected cowpeas<br />

77

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