PNABD246.pdf
PNABD246.pdf
PNABD246.pdf
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particle. In the gl s:house, infection with the virus caused a reduction in<br />
leaf yield of 24% in white, 559 in red, and 36% in subterranean clovers. [AS]<br />
*0280 Shanta, P. ; Menon, K.P.V. 1961. Studies on some properties of the<br />
coconut wilt virus. INDIAN COCONUT JOURNAL, v.15(I):36-46. LEn] [En Abst]<br />
(REP.MB-2398)<br />
Some physical properties and the mode of transmission of coconut wilt virus<br />
(CWV), the virus associated with the root (wilt) disease of coconut has been<br />
studied using cowpea as an indicator plant. The virus is sap trarsmissible and<br />
its insect vector on coconut is Stephanitis typicus Dist. It is also<br />
soil-borne, the clay and silt fractions collected from the base of diseased<br />
trees being infective. It has a fairly wide host range when mechanically<br />
transmitted and occurs naturally in the roots of a number of weeds. The virus<br />
induces 'vein clearing' symptoms on blackgram. [AS]<br />
*0281 Van Velsen, R.J. 1962. Cowpea mosaic, a virus disease of Vigna<br />
sinensis in New Guinea. PAPUA NEW GUINEA AGRICULTURAL JOURNAL,<br />
v.14(4):153-161. [Enj (REP.MB-2743)<br />
The paper describes a mosaic virus disease of cowpea. Vigna mungo and V.<br />
radiata were found susceptible to the virus. Similarities of the present virus<br />
with previously reported mosaic viruses of cowpea are discussed. [EMS]<br />
*0282 Teakle, D.S. ; Gold, A.H. 1963. Further studies of Olpidium as a<br />
vector of tobacco necrosis virus. VIROLOGY, v.19:310-315. [En] [En Abstj<br />
(REP.M-0563)<br />
Mungbean (Vigna radiata) seedlings were used to test tobacco necrosis virus<br />
(TNV) infectivity of zoospore suspensions. When a zoospore suspension of<br />
Olpidium brassicae (Wor.) Dang. and TNV was added to seedling roots of mungbean<br />
and the seedlings were placed in a moist chamber 30 minutes, infection of the<br />
roots by Olpidium and by TNV resulted. When the seedlings were placed in<br />
running tap water, TNV infection was increased. [THH]<br />
*0283 Kassanis, B. ; MacFarlane, I. 1964. Transmission of tobacco necrosis<br />
virus by zoospores of Olpidium brassicae. JOURNAL OF GENERAL MICROBIOLOGY,<br />
v.36:79-93. [EnJ [En Abstj (REP.MB-2394)<br />
Strain D of tobacco necrosis virus (TNV) was transmitted by zoospores of 3<br />
different isolates of Olpidium brassicae to roots of mungbean and lettuce.<br />
Exposure of roots to virus + zoospore mixture for i min. sufficed to infect<br />
them with virus. Roots, exposed to zoospores for 10 min., then washed, were<br />
more readily infected by TNV when virus was introduced during the first hour or<br />
two after zoospore attachment to the root cells than later; there was some<br />
transmission even when virus was withheld till 4 hr after washing. Immersing<br />
roots, inoculated with fungus and virus, in hot water (60 C) killed the fungus<br />
but not the virus, and varying the interval between inoculation and heating<br />
showed that virus became established after 2-3 hr. Virus transmission was<br />
prevented by adding concentrated homologous antiserum to zoospores, or very<br />
dilute antiserum to virus before mixing with zoospores. The extent to which<br />
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