PNABD246.pdf

Blackgram mottle virus (BLMV) isolated from naturally infected mungbean,
blackgram, and soybean plants in Thailand infected plants in the families
Aizoaceae, Amaranthaceae, Chenopodiaceae, Leguminosae, Pedaliaceae and
Solanaceae. The virus was transmitted by beetles (Monolepta signata) and by
mechanical inoculations in the laboratory. The virus in plant sap has a
dilution end point between I.OE-9 and i.OE-10, a thermal inactivation point
between 85 and 90 C for 10 min. and a longevity in vitro of 6-9 weeks at 20 C.
Purified virus preparations had an ultraviolet light absorption spectrum
typical of nucleoprotein components with an A2bO/A280 value of about 1.55.
Purified virus preparations contained isometric particles with a diameter of
about 28 nm. BLMV isolated from mungbean and soybean in Thailand was
serologically identical with the BLMV previously reported from India
(double-diffusion test), but did not react with antisera against some other
beetle-transmitted viruses. [AS/EMS]
- Cucumber Mosaic Virus
*0270 Provvidentt, R. 1976. Reqctiom of Phaseolus and Macroptilium species
to a strain of cucumber moslc virus. PLANT DISEASE REPORTER,
v.60(4):289-293. [En] [En Abstj (REP.MB-0676)
A strain of cucumber mosaic virus (CMV-B) incited unusually prominent
foliar blistering, chlorosis, green vein-banding and deformation resembling
2,4-D injury in naturally and experimentally infected plants of Phaseolus
vulgaris 'Lake Shasta'. Ninety additional domestic cultivars and 105 plant
introductions of the same species reacted with a mild to moderate mottle and/or
green vein-banding. All CMV-B infected plants eventually recovered from
symptoms and recrudescence was rare. CMV-B also infected systemically
accessions of P. aborigenus, P. aconitifolius, Vigna angularis, P. bracteatus,
V. umbellata, P. caracalla, P. cocclneus, P. dumosus, P. erythroloma, P.
lunatus, P. panduratus, P. phyllanthus, P. pilosus, P. polystachios, V.
radiata, Macroptilium atropurpureum, and M. lathyroides. Resistance occurred in
accessions of P. acutifolius var. latifolius, P. adenanthus, P. anisotrichus,
P. polyanthus, and P. trilobatus. CMV-B was efficiently transmitted from bean
to bean by the green peach aphid (Myzus persicae) and carried in 0.3% of the
seeds of the bean cultivar Pioneer. Although of common occurrence in the bean
crops in New York State, CMV-B is rarely noticed because of the mildness and
transient nature of the symptoms in many of the leading commercial cultivars,
and the prevalence of other viruses, particularly bean yellow mosaic virus. [AS]
*0271 Purivirojkul, W. ; Poehlman, J.M. 1977. Injury in mungbean from
natural infection with cucumber mosaic virus. CROP SCIENCE, v.17(4):654-656.
[En] [En Abstj (REP.MB-1758)
Five mungbean (Vigna radiata (L.) Wilczek) strains susceptible to cucumber
mosaic virus (CMV) and one resistant strain were grown with three treatments:
unprotected control; systemic insecticide; and screen cages to exclude insects.
Virus was spread by natural agents. The susceptible strains were damaged
severely in the control and systemic insecticide plots but were free of virus
symptoms when grown in the screen cages. The resistant strain was free of virus
symptoms in all plots. Absence of virus symptoms in susceptible strains in the
cages indicated that the virus was spread by an insect vector. A systemic
insecticide to control the vector provided slight protection to mungbeans from
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