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The Prince. Only Chenopodium quinoa developed systemic chlorotic spots due to<br />

virus infection. The severity of host reaction to MMV infection appeared to be<br />

related to the age at which mungbean became infected. Seven-day old mungbean<br />

seedlings were severely stunted and did not produce seeds. Plants which had<br />

been infected 14 days after seeding were not as seriously affected but growth<br />

remained substantially less than uninfected mungbean plants. This information<br />

is of some value in screening germplasm materials for sources of resistance to<br />

mungbean mottle virus. [ASJ<br />

*0266 Scott, H.A. ; Phatak, H.C. 1979. Properties of blackgram mottle<br />

virus. PHYTOPATHOLOGY, v.69(4):346-348. [En] [En Abst] (REP.MB-2141)<br />

Blackgram mottle virus was purified from infected black valentine bean<br />

plants by a chloroform-butanol procedure. Purified preparations contained<br />

polyhedral particles 28 nm in diameter and exhibited a single<br />

immunoelectrophoretic component that migrated toward the anode at pH 8.8.<br />

Analytical ultracentrifugation showed a single component with a sedimentation<br />

coefficient of 122S and a density of 1.364 gm/cm3. Polyacrylamide gel<br />

electrophoresis demonstrated one nucleic acid component and one coat protein<br />

subunit with estimated molecular weights of 1.4xl.OE6 and 38,200 daltons,<br />

respectively. Phenol-extracted nucleic acid was infectious on pinto bean and<br />

sensitive to ribonuclease and had a nucleotide ratio of G = 24.9%; A = 25.9%; C<br />

= 25.3%; U= 24.0%. Blackgram mottle virus was transmitted by the bean leaf<br />

beetle, Cerotoma trifurcata, and the Mexican bean beetle, Epilachna varivestis.<br />

Reciprocal serologic tests failed to show relationships between blackgram<br />

mottle virus and members of other beetle-transmitted legume virus groups. [ASJ<br />

*0267 Talens, L.T. 1979. Evidence that blackgram mottle and mungbean mottle<br />

viruses are distinct but serologically indistinguishable viruses [Abst.j.<br />

PHILIPPINE PHYTOPATHOLOGY, v.15:89. [Enj (A:PS)<br />

MEETING: Annual Meeting of the Philippine Phytopathological Society, 16th --<br />

Manila, Philippines, May 2-5, 1979<br />

Comparative studies on the host range, symptomatology, and serology of<br />

blackgram mottle virus (BGKV) and mungbean mottle virus (MMV) suggest that the<br />

two viruses are serologically indistinguishable, but biologically distinct.<br />

They only differed in symptoms induced on the indicator plants Chenopodium<br />

amaranticolor and C. quinoa. [EMSj<br />

*0268 Scott, H.A. ; Hoy, .W. 1981. Blackgram mottle virus. DESCRIPTIONS<br />

OF PLANT VIRUSES, Set.15(..o.237). [En] (A:PS)<br />

Geographical distribution, host range, symptomatology, and other<br />

information on blackgram mottle virus are described. [EMS]<br />

*0269 Honda, Y. ; Iwaki, M. ; Thongmeearkom, P. ; Deema, N. ; Srithongchai,<br />

W. 1982. Blackgram mottle virus occurring on mungbean and soybean in Thailand.<br />

JARQ: JAPAN AGRICULTURAL RESEARCH QUARTERLY, v.16(I):72-77. [EnJ [En Abst]<br />

(A:PS)<br />

69

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