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were identified as natural hosts. In microprecipitin and agar gel diffusion<br />

tests, the virus failed to react with antisera of bean common mosaic, bean<br />

yellow mosaic, soybean mosaic, potato virus Y, peanut mottle, henbane mosaic,<br />

and tobacco etch viruses. This causal virus, apparently in the potato virus Y<br />

group, is a newly-characterized pathogen of mungbean and is named mungbean<br />

mottle virus. [AS]<br />

*0263 Singh, R.N. ; Nene, Y.L. 1978. Further studies on the mosaic mottle<br />

disease of urd bean. INDIAN PHYTOPATHOLOGY, v.31(2):159-162. (REP.MB-2175)<br />

A viral disease with systemic mottle symptoms in blackgram (Vigna mungo) is<br />

widely prevalent in north India. The in vitro properties of the virus in<br />

extract are: thermal inactivation, infection after heating for 10 minutes at 55<br />

C but not at 60 C; dilution end point, infection at 5 x I.OE-3 but not at<br />

I.OE-4; longevity in vitro at 24 C, infection at 36 hours but not at 48 hours.<br />

The host range is restricted to a few legumes. It is transmitted by Aphis<br />

craccivora and A. gossypli and is serologically related to common bean mosaic<br />

virus. [AS]<br />

*0264 Talens, L.T. 1978. Mungbean viruses in the Philippines. I. Identity<br />

of a virus causing mottle in mungbean. PHILIPPINE PHYTOPATHOLOGY,<br />

v.14(1&2):58-b2. [Enj (REP.MB-2U79)<br />

A virus inducing a mottle reaction and stunted growth in field-grown<br />

mungbean [Vigna radiata (L.) Wilczekj was isolated and identified. Based on<br />

thermal stability, serological property, and morphological features, the<br />

mungbean virus isolate was found to possess physical and antigenic properties<br />

similar to blackgram mottle virus (BgMV). The virus was stable at high<br />

temperature (10 min duration at 90-95 C) and occurred in high concentration<br />

(infecttvity retained at I.OE-6 dilution) in infected mungbean tissues<br />

harvested 10-14 days after mechanical inoculation. The virus reacted strongly<br />

in an Ouchterlony double immunodiffusion test with reference BgMV antiserum.<br />

Isometric particles which measured 28-30 nm in diameter were seen in electron<br />

microscopic examinations of partially purified preparations stained with 2%<br />

neutral phosphotungsta-e. This study describes the first report on the<br />

occurrence of mungbean mottle virus in the Philippines with antigenic<br />

properties similar to blackgram mottle virus. [ASJ<br />

*0265 Talens, L.T. ; Dolores, A.C. 1978. Mungbean viruses in the<br />

Philippines. 2. Host range, symptomatology and effect of mungbean mottle virus<br />

on growth of mungbean, Vigna radiata (L.) Wilczek. PHILIPPINE JOURNAL OF CROP<br />

SCIENCE, v.3(4):242-246. [En] [En Abst] (REP.MB-2085)<br />

A virus previously named mungbean mottle virus (MMV) was shown to incite<br />

veinal chlorotic reaction in inoculated primary leaves of mungbean. This was<br />

accompanied by the appearance of random chlorotic areas in the emerging<br />

trifoliate leaves. The chlorotic reaction originated from the veins, spread<br />

outwards co the interveinal areas, and gave the foliage a mottled appearance.<br />

The chlorotic reaction intensified as viral infection progressed. This is<br />

apparently the first detailed description of the virus in the Philippines.<br />

Blackgram and soybean proved susceptible to systemic invasion by the mottle<br />

virus. Local lesions were produced on Chenopodium amaranticolor, C. quinoa,<br />

Cyamopsis tetragonoloba, Macroptilium lathyroides, and Phaseolus vulgaris cv.<br />

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