PNABD246.pdf
PNABD246.pdf
PNABD246.pdf
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*0221 Bhaktavatsalam, G. ; Nene, Y.L. ; Beniwal, S.P.S. 1983.<br />
Ultrastructural changes in urdbean leaves infected by urdbean leaf crinkle<br />
virus. INDIAN PHYTOPATHOLOGY, v.3b(2):228-232. [En] [En Abstj (A:PS)<br />
Virus-like particles (VLP) appeared to be present in blackgram leaf crinkle<br />
virus (BLCV)-infected blackgram leaves in the nucleus, cytoplasm and<br />
chloroplasts. Healthy cells were devoid of such particles. The VLP appeared to<br />
be spherical with a diameter of 25 tu 30 nm. Hypertrophy of infected cells was<br />
observed. Some mitochondria were observed to be filiform as compared to normal<br />
spherical ones in healthy cells. [AS]<br />
*0222 Bhaktavatsalam, G. ; Nene, Y.L. ; Beniwal, S.P.S. 1983. Influence of<br />
certain physico-chemical factors on the infectivity and stability of urdbean<br />
leaf crinkle virus. INDIAN PHYTOPATHOLOGY, v.36(3):489-493. [En] [En Abstj<br />
(A:PS)<br />
Infectivity and longevity of blackgram leaf crinkle virus (BLCV) were<br />
affected by a number of physicochemical factors. The infectivity was pH<br />
dependent with maximal infectivity at 7.8. Thermal inactivation of BLCV<br />
appeared to be highly pHldependent. Calcium tribasic and dibasic phospates and<br />
EDTA completely inhibited virus infectivity whereas magnesium silicate had no<br />
effect on it. Actinomycin D and cycloheximide decreased infectivity whereas<br />
tetracycline hydrochloride did not affect it. Phenol treatment considerably<br />
reduced viral infectivity index suggesting that BLCV is not a viroid. BLCV is<br />
ether-insensitive and does not possess lipids. Addition of 5X sucrose plus 1%<br />
mercaptoethanol to inoculum increased infectivity as well as longevity of BLCV.<br />
[AS]<br />
*0223 Chowdhury, A.K. ; Nath, P.S. 1983. A rapid method of inoculation of<br />
urid bean leaf crinkle virus (ULCV) using germinated seed [Abst.j. INDIAN<br />
JOURNAL OF EXPERIMENTAL BIOLOGY, v.21(3):58. [En] [En] (REP.MB-2450)<br />
Blackgram leaf crinkle virus was inoculated through germinated seeds by<br />
shaking the seeds with a virus preparation for different time intervals. Plants<br />
developed from the inoculated seeds showed virus symptoms and the rate of<br />
transmission efficiency varied with the exposure of germination and shaking<br />
period. [AS]<br />
*0224 Dubey, G.S. ; Sharma, I. ; Prakash, N. 1983. Some properties of<br />
uridbean leaf crinkle virus. INDIAN PHYTOPATHOLOGY, v.3b:762-764. [En]<br />
(A:PS)<br />
BLCV was successfully transmitted by Aphis craccivora and Acyrthosiphon<br />
pisum. Electron microscopic observations revealed the particles to be isometric<br />
with an average diameter of 320 A. The virus did not show any serological<br />
relationship with any of 11 viruses of the bromo, poty and como virus groups<br />
attacking other legume species. [EMS]<br />
*0225 Kadian, O.P. 1983. Occurrence and incidence of leaf crinkle disease<br />
on urdbean and mungbean in Haryana. HARYANA AGRICULTURAL UNIVERSITY JOURNAL<br />
OF RESEARCH, v.13(1):121-126. (REP.MB-2578)<br />
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