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*0201 Narayanasamy, P. ; Jaganathan, T. 1974. Characterisation of blackgran<br />

leaf crinkle virus. MADRAS AGRICULTURAL JOURNAL, v.61(10/12):979-982. tEn]<br />

[En Abst] (A:PS)<br />

The blackgram leaf crinkle virus (BLCV) lost its activity at dilutions<br />

above 1:5000, at temperatures above 60 C and after 48 hours in storage at room<br />

temperature. The optimum pH was found to be 7.2. BLCV was antigenic, and<br />

capable of reacting specifically with its antiserum. Of the 24 plant species<br />

tested cowpea, cluster beans, mungbean, groundnut and redgram were infected by<br />

BLCV. [AS]<br />

*0202 Dhingra, K.L. 1975. Transmission of urid bean leaf crinkle virus by<br />

two aphid species. INDIAN PHYTOPATHOLOGY, v.28:80-82. [En] [En Abst] (A:PS)<br />

Aphis craccivora Koch. and Aphis gossypii Glov. proved to be the vectors of<br />

a virus which caused crinkling of the leaves in blackgram (Vigna mungo Hepper).<br />

A very short acquisition feeding period of 30 seconds to two minutes preceded<br />

by a pre-acquisition fasting was found necessary for the successful<br />

transmission of virus. The causal virus has close resemblance in symptomatology<br />

to blackgram leaf crinkle virus reported earlier. [AS]<br />

*0203 Kolte, S.J. ; Nene, Y.L. 1975. Host range and properties of urd bean<br />

leaf crinkle virus. INDIAN PHYTOPATHOLOGY, v.28:430-431. [En] (A:PS)<br />

All 52 plant species belonging to 10 different families were tested for<br />

reaction to blackgram leaf crinkle virus. Out of All 52 plant species, only 3<br />

plant species; viz., Vigna radiata, V. aconitifolia, and V. sinesis were<br />

susceptible. The virus was found to be inactivated when sap from the infected<br />

leaves was subjected to temperature ranging from 60 to 70 C for 10 minutes, or<br />

when sap was diluted above 1.0E-4. Infectivity of the sap was retained for 3<br />

days at room temperature, but was lost on the 9th day refrigeration (5 C). This<br />

virus is considered to be distinct from those reported so far on blackgram.<br />

[THHJ<br />

*0204 Narayanasamy, P. ; Jaganathan, T. 1975. Effect of black gram leaf<br />

crinkle virus infection on seed set and distribution of virus in the seeds.<br />

MADRAS AGRICULTURAL JOURNAL, v.62(3):151-154. [EnJ [En Abst] (A:PS)<br />

Blackgram leaf crinkle virus induced pollen sterility which ranged from<br />

13.64 to 72.09% depending on the variety of blackgram. The significance of<br />

pollen sterility as a limiting factor of yield is discussed. The study on<br />

distribution of BLCV in the seeds of blackgram revealed that the virus was<br />

present in the plumule, radicle and cotyledons of the germinating seed but not<br />

in the seed coat. The virus was found at a higher frequency in the plumule. [AS]<br />

*0205 Narayanasamy, P. ; Jaganathan, T. 1975. Studies on seed transmission<br />

of black gram leaf crinkle virus: effect of age of plants at infection.<br />

MADRAS AGRICULTURAL JOURNAL, v.62(5):287-290. [En] [En AbstJ (REP.MB-0674)<br />

The experiments with blackgram plants inoculated with blackgram leaf<br />

crinkle virus (BLCV) at 5-day intervals from 5 to 50 days of age showed that<br />

52

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