PNABD246.pdf

MYMV isolated from Thailand and BGMV from Puerto Rico are both
geminiviruses with somewhat different host ranges. The antisera against the two
viruses absorbed with freeze-dried powder of healthy components and purified
through the same method of virus purification from kidney bean leaves, were
applied to dot immunobinding assay (DIBA). In case of using dilutions of the
antiserum against MYMV at 1:800 and 1:1,600, 0.4, 4 ng of MYMV, and 0.7, 7 ng
of BGMV were able to be detected by DIBA. In DIBA, antiserum against MYMV, and
antiserum against BGMV reacted with MYMV or BGMV in crude saps of diseased
leaves in any combination. The DIBA detected the viruses directly from diseased
leaves, but could not differentiate between viruses. One protein with MW 27,500
was isolated from MYMV by SDS-polyacrylamide electrophoresis. Antiserum against
MYMV and antiserum against BGMV showed reaction with MYMV or BGMV in
immunoblotting assay. It we clarified that MYMV and BGMV had common antigens
according to the above-mentioned experimental results. [HGS]
- Leaf Crinkle Virus
*0198 Kolte, S.J. ; Nene, Y.L. 1972. Studies on symptoms and mode of
transmission of the leaf crinkle virus of urd bean (Phaseolus mungo). INDIAN
PHYTOPATHOLOGY, v.25(3):401-404. [En] (REP.MB-0193)
Evidence has been presented to show that the leaf crinkle disease of
blackgram is caused by a mechanically transmissible virus which is also
seed-borne. The symptoms of the disease, described in this paper in more
detail, are characterized by the stunting of plants and crinkling of leaves.
Affected plants produce very few pods. The disease, at present, is not
widespread. [AS]
*0199 Narayanasamy, P. ; Jaganathan, T. 1973. Vector transmission of black
gram leaf crinkle virus. MADRAS AGRICULTURAL JOURNAL, v.60(7):651-652. [En]
(REP.MB-1309)
Whitefly (Bemisia tabaci) was tested for its ability to transmit the
biackgram leaf crinkle virus. The percentage of transmission in different
experiments varied from 20 to 60%. The incubation period of the virus in plants
ranged from 20 to 23 days. No incubation period of the virus in the vector is
necessary. [THH]
*0200 Gupta, V.K. 1974. Leaf crinkle, a virus disease of Phaseolus --ngo L.
in Himachal Pradesh. INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY, v.12(5):477-478.
[EnJ [En AbstJ (REP.MB-1568)
Leaf crinkle and phyllody disease of V. mungo (blackgram) can be
transmitted by grafting method and through seeds, in addition to sap
transmission. The virus infects Phaseolus vulgaris, V. radiata and Nicotiana
tabacum. The virus is seed borne. The thermal inactivation point of the virus
is between 70 and 75 C, dilution end point between 1.OE-3 and 1.OE-4 and it is
infectious at room temperature up to 96 hr. [AS]
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