PNABD246.pdf

virus. MICROBIOLOGY AND IMMUNOLOGY, v.29(8):783-789. [Enj (REP.MB-2767)
This is the first report on characterization of the mungbean yellow mosaic
virus (MYMV) nucleic acid. The nucleic acid isolated from MYMV had an UV
spectrum with 260/230 nm and 260/280 nm ratios of about 2.5 and 2.0,
respectively, and gave a positive diphenylamine reaction indicating the
presence of deoxyribose. A 5-nm shift in wavelength max. and approximately 18%
hyperchromicity were observed within 10 min after addition of formaldehyde to
purified MYMV DNA, whereas calf thymus DNA, which is double stranded, did not
show such a shift. The UV light absorption of MYMV DNA increased over a broad
range of temp. from 20 to 70 C, whereas calf thymus DNA showed a sharp
transition with a Tm of 77 C. Nucletc acid preparation from MYMV analyzed by
polyacrylamide gel electrophoresis i.1 7 m urea exhibited two bands. MYMV
nucleic acid was hydrolyzed by DNase I but not by bovine pancreatic RNase A.
When the susceptibiiity of MYMV nucleic acid to nuclease SI was tested, the
rate of digestion of MYMV nucleic acid was comparable to that of heat-denatured
calf thymus DNA. The results suggest that the nucleic acid of MYMV is ssDNA.
[PLH]
*0196 Honda, Y. ; Iwaki, M. ; Thongmeearkom, P. ; Kiratiya-Angul, K. ;
Kiratiya-Angul, S. ; Srithongchai, W. ; Prommin, M. ; Kittipakorn, K. ;
Sarindu, N. ; Deema, N. ; Syamananda, R. ; Hibi, T. ; Saito, Y. 1986. Mungbean
yellow mosaic virus isolated from mungbean in Thailand. TROPICAL AGRICULTURE
RESEARCH CENTER, TECHNICAL BULLETIN, no.21:189-202. [En] [En Abst] (A:PS)
The whitefly-borne mungbean yellow
transmitted
mosaic virus
by mechanical
(MYMV) in
inoculation.
Thailand was
Among the several buffers
attempted
used
transmissions,
in
0.I M potassium or
highest
sodium phosphate,
transmission pH
rates.
7.8, gave
The optimal
the
incubation temperatures
expression
for
ranged
symptom
from 25 to 30 C in the growth
and
chamber
20 C at
or
night
30 C
in
in
the
the
greenhouse.
daytime
Host range of
plant
MYMV
species
was limited
in the
to
family
seven
Leguminosae. Determinations
virus in plant
of the
sap gave
stability
the following
of the
results: thermal inactivation
40-50 C for 10
point
min,
of
dilution end point of
vitro
1.OE-2
of 1-2
- I.OE-3,
days at
and
20
longevity
C. Purified
in
virus preparations had
absorption
an ultraviolet
spectrum
light
typical of that of
about
nucleoprotein
1.3-1.4. Purified
with a A260/A280
preparations value
and
of
leaf-dip samples contained
particles
geminate
about 18 X 30 nm in size. Infectivity
presence of
was
purified
associated
virus
with
particles.
the
In thin sections, the
were
virus
isometric,
particles
about 15-20 nm in diameter,
aggregates
and they
that
often
sometimes
formed loose
almost filled completely the nucleus
phloem
of
cells.
infected
Mungbean infected by whitefly
hypertrophied
transmission
nucleoli,
or by grafting
aggregates showed
of virus particles, and fibrillar
the nuclei
bodies
of phloem
in
cells as early as
vacuoles
2 days before
or lumens
symptom
of the
appearance.
partially In
or fully differentiated
elements,
infected
virus
sieve
particles occasiona.ly formed aggregates
cylindrical having
arrangement
a double
of particles. No virus particles
tissues
were
other
detected
than
in
the phloem of infected plants or in any tissues of
comparable healthy plants. [AS]
*0197 Honda, Y. 1986. [Serological detection of mung bean yellow mosaic
virus (HYHV) and bean golden mosaic virus and their taxonomic relationshipj.
PHYTOPATHOLOGICAL SOCIETY OF JAPAN, ANNALS, v.52(3):553-554. [Jaj (A:PS)
(NOTE: Text in Jap., translated into Eng. by H.G. Su)
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