PNABD246.pdf

Deema, N. 1983. Mechanical transmission, purification, and some properties of
white-fly-borne mung bean yellow mosaic virus in Thailand. PLANT DISEASE,
v.67(7):801-804. [En] [En Abst] (A:PS)
The whitefly-borne mungbean yellow mosaic virus (MYMV) in Thailand was
transmitted by mechanical inoculation. Among the several buffers used in
attempted transmissions, 0.1 M potassium or sodium phosphate, pH 7.8, gave the
highest transmission rates. The optimal incubation temperatures for symptom
expression ranged from 25 to 30 C in the growth chamber or 30 C in the daytime
and 20 C at night in the greenhouse. Host range of MYMV was limited to seven
plant species in the family Leguminosae. Determinations of the stability of the
virus in plant sap gave the following results: thermal inactivation point of
40-50 C for 10 min, dilution end point of i.OE-2 to 1.OE-3, and longevity in
vitro of 1-2 days at 20 C. Purified virus preparations had an ultraviolet light
absorption spectrum typical of that of aucleoprotein with a A260/A280 value of
about 1.3-1.4. Purified preparations and leaf-dip samples contained geminate
particles of about 18 x 30 nm. Infectivity was associated with the presence of
purified virus particles. [ASJ
*0192 Singh, R.N. 1983. Yellow mosaic of Phaseolus atropurpureus caused by
mungbean yellow mosaic virus. LEGUME RESEARCH, v.6(l):52. [En] (A:PS)
Phaseolus atropurpureus, a pasture legume, is reported as a host to
mungbean yellow mosaic virus. [THH]
*0193 Ikegami, M. ; Morinaga, T. ; Miura, K. 1984. Infectivity of
virus-specific double-stranded DNA from tissue infected by mung bean yellow
mosaic virus. VIRUS RESEARCH, v.1(6):507-512. [En] [En Abst] (REP.MB-2676)
A double-stranded (ds) DNA which may be a replicative intermediate v;s
isolated from bean (Phaseolus vulgaris L. 'Top Crop') leaves systemically
infected with mungbean yellow mosaic virus. The isolation method used
phenol/chloroform extraction, hydroxyapatite column chromatography, and
rate-zonal centrifugation. The dsDNA was a circular molecule and had sequences
complementary to those of viral DNA. These molecules infected bean plants and
typical symptoms of downward curling appeared. [AS]
*0194 Ram, R.S. ; Chenulu, V.V. ; Sastry, L.V.S. 1984. Metabolic changes in
black gram infected with mung yellow mosaic virus. INDIAN PHYTOPATHOLOGY,
v.37(1):88-92. [En] [En Abst] (A:PS)
Mungbean yellow mosaic virus (MYMV) infection in blackgram (Vigna mungo)
caused significant reduction in the total dry matter content of plants,
chlorophylls and carotenoids in the leaf tissues of resistant (S-1) as well as
susceptible (Krishna) varieties. Reduction in total chlorophyll was, however,
more severe in the susceptible variety. The chlorophyll content decreased
progressively with the increase in disease severity and was due to reduced
synthetic activity of the chlorophyllase enzyme. [AS]
*0195 Ikegami, M. ; Yazaki, K. ; Honda, Y. ; Iwaki, M. ; Fujii, H.
Morinaga, T. ; Miura, K. 1985. Single-stranded DNA in mung bean yellow mosaic
49