Tour-de-Force
Tour-de-Force Tour-de-Force
Tour-de-Force: Interplay between Mitochondria and Cell Cycle Progression Fall 2007Financial Overview and Time LineProductPriceBlotting & Co-IP Kits € 4,822.00Cell Lines € 8,000.00Fractionation Kits € 545.00Mutations & Mutation Kits € 3,615.00Primary Antibodies € 5,838.00Proteins € 2,660.00Real Time PCR € 1,347.00Secondary Antibodies € 382.33siRNA € 1,831.12Staining € 1,322.0010% unexpected costs € 3,000.00Total € 33,362.45Annual Stipend Researcher € 40,000.00Total € 160,000.00Total Cost Research € 193,362.45Year 1 Year 2 Year 3 Year 4Hypothesis 1Hypothesis 2Hypothesis 3Hypothesis 4Hypothesis 5SCI 332 Advanced Molecular Cell Biology Research Proposal 90
Tour-de-Force: Interplay between Mitochondria and Cell Cycle Progression Fall 2007AcknowledgementsFirst of all, we would like to thank dhr. dr. F.A.C. Wiegant and dhr. prof. dr. J. Boonstra for their devotionto this project. They have been of great help to us.Secondly, we greatly appreciated the help of dhr.dr. L.H.K. Defize. He has provided us with great idea’sand advice on the methodology used in this program.Ms. Martha Lewis, of Santa Cruz Biotechnologies, advised us with choosing the antibodies that wereproposed in this project. We want to thank her for that.Dr. Cristian Scheler, of ProteomeFactory has given great advice on the possibilities of protein sequencing,and thus we thank him for that.Ms. Silvia Otternberg, of Thermo Scientific Pierce Protein Research Products, has provided detailedinformation on the use of cell fractionation to which we are thankful.Mr. Rizwan Farooqui, Thermo Scientific Pierce Protein Research Products, for providing information onCo immunoprecipitation ,which was of great help.Dr. Jamil Talhouk , Of Biovision. He provided our research with additional insight on cell fractionation.Lastly, Ms. Belin Chen, from Abnova, has been a great source of information on the usage of a Mfn2 C-terminal antibody. We are very thankful.SCI 332 Advanced Molecular Cell Biology Research Proposal 91
- Page 39 and 40: Tour-de-Force: Interplay between Mi
- Page 41 and 42: Tour-de-Force: Interplay between Mi
- Page 43 and 44: Tour-de-Force: Interplay between Mi
- Page 45 and 46: Tour-de-Force: Interplay between Mi
- Page 47 and 48: Tour-de-Force: Interplay between Mi
- Page 49 and 50: Tour-de-Force: Interplay between Mi
- Page 51 and 52: Tour-de-Force: Interplay between Mi
- Page 53 and 54: Tour-de-Force: Interplay between Mi
- Page 55 and 56: Tour-de-Force: Interplay between Mi
- Page 57 and 58: Tour-de-Force: Interplay between Mi
- Page 59 and 60: Tour-de-Force: Interplay between Mi
- Page 61 and 62: Tour-de-Force: Interplay between Mi
- Page 63 and 64: Tour-de-Force: Interplay between Mi
- Page 65 and 66: Tour-de-Force: Interplay between Mi
- Page 67 and 68: Tour-de-Force: Interplay between Mi
- Page 69 and 70: Tour-de-Force: Interplay between Mi
- Page 71 and 72: Tour-de-Force: Interplay between Mi
- Page 73 and 74: Tour-de-Force: Interplay between Mi
- Page 75 and 76: Tour-de-Force: Interplay between Mi
- Page 77 and 78: Tour-de-Force: Interplay between Mi
- Page 79 and 80: Tour-de-Force: Interplay between Mi
- Page 81 and 82: Tour-de-Force: Interplay between Mi
- Page 83 and 84: Tour-de-Force: Interplay between Mi
- Page 85 and 86: Tour-de-Force: Interplay between Mi
- Page 87 and 88: Tour-de-Force: Interplay between Mi
- Page 89: Tour-de-Force: Interplay between Mi
- Page 93 and 94: Tour-de-Force: Interplay between Mi
- Page 95 and 96: Tour-de-Force: Interplay between Mi
- Page 97 and 98: Tour-de-Force: Interplay between Mi
- Page 99 and 100: Tour-de-Force: Interplay between Mi
- Page 101 and 102: Tour-de-Force: Interplay between Mi
- Page 103 and 104: Tour-de-Force: Interplay between Mi
- Page 105 and 106: Tour-de-Force: Interplay between Mi
- Page 107 and 108: Tour-de-Force: Interplay between Mi
- Page 109 and 110: Tour-de-Force: Interplay between Mi
- Page 111 and 112: Tour-de-Force: Interplay between Mi
- Page 113 and 114: Tour-de-Force: Interplay between Mi
- Page 115 and 116: Tour-de-Force: Interplay between Mi
- Page 117 and 118: Tour-de-Force: Interplay between Mi
<strong>Tour</strong>-<strong>de</strong>-<strong>Force</strong>: Interplay between Mitochondria and Cell Cycle Progression Fall 2007Financial Overview and Time LineProductPriceBlotting & Co-IP Kits € 4,822.00Cell Lines € 8,000.00Fractionation Kits € 545.00Mutations & Mutation Kits € 3,615.00Primary Antibodies € 5,838.00Proteins € 2,660.00Real Time PCR € 1,347.00Secondary Antibodies € 382.33siRNA € 1,831.12Staining € 1,322.0010% unexpected costs € 3,000.00Total € 33,362.45Annual Stipend Researcher € 40,000.00Total € 160,000.00Total Cost Research € 193,362.45Year 1 Year 2 Year 3 Year 4Hypothesis 1Hypothesis 2Hypothesis 3Hypothesis 4Hypothesis 5SCI 332 Advanced Molecular Cell Biology Research Proposal 90