Tour-de-Force

Tour-de-Force: Interplay between Mitochondria and Cell Cycle Progression Fall 2007Although the majority of the studies on cyclin D effects on NRF-1 have been focused on cdk4, thisdoes not exclude cdk6 as another possible mediator. By using co-immunoprecipitation we can see whenwhich cyclin D isoform binds to which cdk.Possible role of other cdk’s in mediating regulation of NRF-1 and NRF-2 activityDue to the fact that it is cdk4 that is responsible for the phosphorylation of NRF-1 it would be interesting tosee if other cdk’s are also involved in this regulation during the cell cycle.We would therefore first like to check if these cdks can interact with NRF-1 and 2. In order to do this wewould like to do kinase assay using the AlphaScreen® PhosphoSensor Kits (PerkinElmer).SCI 332 Advanced Molecular Cell Biology Research Proposal 68