Annex White book.pdf

Picture 7. Chromatogram at 432 nm for a multipattern solution of 4 mg/l. The separation sequenceis Red Sudan G, Sudan I, Sudan II, Sudan III, RedSudan 7B and Sudan IVA4.3.4. Microscopic analysisThis method is applied on saffron threads or saffron powder in orderto determine whether the sample consists solemnly of Crocus sativusL. stigmas or if other flower residue and foreign material are alsopresent.The preparations for microscopic analysis are performed accordingto ISO 3632, section 6. The elements that should be found in order toverify the sole existence of Crocus sativus L. stigmas are as follows:• Upper end stigma residue with epitheliums• Epidermal stigma residue• Epidermal column residue• Pollen granules with a 80 – 100 μm diameter• Transport container debris• Stamens residue• Starch granules• Inorganic material• Foreign material cellular residue• Cells with content that remains colored167