EnteroPluri-Test - Liofilchem
EnteroPluri-Test - Liofilchem
EnteroPluri-Test - Liofilchem
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<strong>EnteroPluri</strong>-<strong>Test</strong>ReadEnterobacteriaceae andother oxidase-negative Gram-negativchem ® chem company logo are registered trademarks of LIOFILCHEM s.r.l. Pakaging <strong>EnteroPluri</strong>-<strong>Test</strong>10 test7861825 test78619VP TEST EP100/200 test 80281K VKOVACS’ REAGENT800/1000 test 80271<strong>EnteroPluri</strong>-<strong>Test</strong> CODE BOOK 1 book 71709chem ®V Te) ItalyTFRevPrinted in Italy
Enterobacteriaceaee di altri batteri gram negativi, ossidasi negativiIdentification system of Enterobacteriaceae and othergram negative – oxidase negative bacteriaLIOFILCHEM Bacteriology Products<strong>Test</strong> procedure1 2 3chem ® chem company logo are registered trademarks of LIOFILCHEM s.r.l.Remove both caps.Pick a well isolated colony directly with thetip of the inoculating wire.Inoculate <strong>EnteroPluri</strong>-<strong>Test</strong> bwire, then withdrawing wire through alltwelve compartments applying a turningmotion4 5 6The portion of the wire remaining in the tubemaintains anaerobic conditions necessary fortrue fermentation of glucose, production of asand decarboxylation of lysine and ornithine.78With the broken off part of the wire, punchholes through the foil covering the air inletsof the last eight compartments (adonitol,lactose, arabinose, sorbitol, Voges-Proskauer,dulcitol/PA, urea and citrate).Indole testReinsert wire into <strong>EnteroPluri</strong>-<strong>Test</strong>, using aturning motion through all twelvecompartments, until the notch on the wire isaligned with the opening of the tube.Replace both caps.Incubate at 35 to 37° C for 18 to 24 hourswith <strong>EnteroPluri</strong>-<strong>Test</strong>or in an upright position. Allow for aircirculation between incubated tubes.9.<strong>EnteroPluri</strong> – <strong>Test</strong> ...MANUALE DEI CODICICODEBOOKVoges-Proskauer testInterpret and record all reactions withexception of indole and Voges-Proskauer.By using a syringe inject 3-4 drops of Kovacs’reagent in the H 2S/Indole sector.By using a syringe inject 3 drops of alphanaphtoland 2 drops of potassium hydroxidein the VP sector.Form the 5-digit code following theinstructions and identify the microorganismusing the Codebook.chem ® ref. 6552266Rev. 1 - 16/04/2013chem 2013Printed in ItalyVia Scozia zona ind.le, 64026 Roseto degli Abruzzi (Te) ItalyTel. +39 0858930745 Fax +39 0858930330 wwwccchem.net
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