Regis Chiral Columns - Canadian Life Science

REGIS TECHNOLOGIESPirkle PhasesWhelk-01, Whelk-02Whelk-01 is a widely applicable chiral phase, due to the incorporationof both π-acceptor and π-donor characteristics. It was originallydesigned for the separation of underivatized non-steroidalanti-inflammatory drugs, but shows versatility in the analysis of awide range of compounds, including amides, epoxides, esters,ureas, carbamates, ethers, aldehydes, ketones, carboxylic acidsand alcohols. Figure 1 shows the separation of warfarin enantiomersusing both normal and reversed-phase modes.Figure 1. Separation of warfarinColumn: (R,R)-Whelk-01 (250mm x 4.6mm)Flow rate: 1ml/minNormal-phase eluent:Hexane - IPA (65:35)+0.1% CH 3 COOHRun time: 11.5 minsK' 1 : 1.54α: 2.07Reversed-phase eluent:CH 3 OH - H 2 O (70:30)+0.1% CH 3 COOHRun time: 15.0 minsk' 1 : 3.54α: 1.55Whelk-02 is the trifunctional version of the Whelk-01. It showssimilar selectivity but enhanced stability with strong organicmodifiers.Figure 2. Separation on (S,S)-ULMOULMOThe ULMO chiral stationary phase has the general capability ofseparating enantiomers of many racemate classes, and is particularlygood at separating enantiomers of aryl carbinols(see Figure 2).Sample: Phenyl isopropyl carbinolColumn: (S,S)-ULMO(250mm x 4.6mm)Eluent: Heptane - IPA (99:1)Flow rate: 1ml/minRun time: 6 minsk' 1 : 0.86α: 1.38Figure 3. Separation on (S,S)-DACH-DNBDACH-DNBThe latest addition to the Regis chiral range is the DACH-DNBphase, which contains the 3,5-dinitrobenzoyl derivative of trans1,2-diaminocyclohexane. It can resolve a wide range of compoundclasses, including sulphoxides, phosphine oxides,selenoxides, organometallics and atropisomers (see Figure 3).Sample: SulphoxideColumn: (S,S)-DACH-DNB(250mm x 4.6mm)Eluent: CH 2 Cl 2 - IPA (98:2)Flow rate: 1ml/minRun time: 17 minsk' 1 : 3.33α: 1.63218Toll Free: 1-888-226-2775 E-mail: info@lifescience.ca Web: www.lifescience.ca