Regis Chiral Columns - Canadian Life Science

REGIS TECHNOLOGIESPirkle PhasesWhelk-01, Whelk-02Whelk-01 is a widely applicable chiral phase, due to the incorporationof both π-acceptor and π-donor characteristics. It was originallydesigned for the separation of underivatized non-steroidalanti-inflammatory drugs, but shows versatility in the analysis of awide range of compounds, including amides, epoxides, esters,ureas, carbamates, ethers, aldehydes, ketones, carboxylic acidsand alcohols. Figure 1 shows the separation of warfarin enantiomersusing both normal and reversed-phase modes.Figure 1. Separation of warfarinColumn: (R,R)-Whelk-01 (250mm x 4.6mm)Flow rate: 1ml/minNormal-phase eluent:Hexane - IPA (65:35)+0.1% CH 3 COOHRun time: 11.5 minsK' 1 : 1.54α: 2.07Reversed-phase eluent:CH 3 OH - H 2 O (70:30)+0.1% CH 3 COOHRun time: 15.0 minsk' 1 : 3.54α: 1.55Whelk-02 is the trifunctional version of the Whelk-01. It showssimilar selectivity but enhanced stability with strong organicmodifiers.Figure 2. Separation on (S,S)-ULMOULMOThe ULMO chiral stationary phase has the general capability ofseparating enantiomers of many racemate classes, and is particularlygood at separating enantiomers of aryl carbinols(see Figure 2).Sample: Phenyl isopropyl carbinolColumn: (S,S)-ULMO(250mm x 4.6mm)Eluent: Heptane - IPA (99:1)Flow rate: 1ml/minRun time: 6 minsk' 1 : 0.86α: 1.38Figure 3. Separation on (S,S)-DACH-DNBDACH-DNBThe latest addition to the Regis chiral range is the DACH-DNBphase, which contains the 3,5-dinitrobenzoyl derivative of trans1,2-diaminocyclohexane. It can resolve a wide range of compoundclasses, including sulphoxides, phosphine oxides,selenoxides, organometallics and atropisomers (see Figure 3).Sample: SulphoxideColumn: (S,S)-DACH-DNB(250mm x 4.6mm)Eluent: CH 2 Cl 2 - IPA (98:2)Flow rate: 1ml/minRun time: 17 minsk' 1 : 3.33α: 1.63218Toll Free: 1-888-226-2775 E-mail: info@lifescience.ca Web: www.lifescience.ca

ORDERING INFORMATION - REGIS TECHNOLOGIESOrdering Information - Regis PhasesColumn Dimensions 2 (mm)Regis Phase50 x 2.1 50 x 4.6 250 x 4.6 250 x 105µm 1 $1076 $1076 $1794 $5978(R,R)-α-Burke 2 RG731602 RG731613 RG735035 RG735235(S,S)-α-Burke 2 RG731603 RG731614 RG735037 RG735237$1025 $1025 $1794 $5978(R,R)-β-Gem 1 RG731600 RG731611 RG731043 RG731243(S,S)-β-Gem 1 RG731601 RG731612 RG731029 RG731229(3R,4S)-Pirkle 1-J RG731604 RG731615 RG731044 RG731244(3S,4R)-Pirkle 1-J RG731605 RG731616 RG731045 RG731245$1076 $1076 $1913 $6575(R,R)-DACH-DNB RG788246 RG788248 RG788101 RG788102(S,S)-DACH-DNB RG788247 RG788249 RG788201 RG788202(R,R)-ULMO RG787648 RG787650 RG787200 RG787201(S,S)-ULMO RG787647 RG787649 RG787100 RG787101(R,R)-Whelk-01 RG786901 RG786905 RG786201 RG786202(S,S)-Whelk-01 RG786900 RG786904 RG786101 RG786102(R,R)-Whelk-01 3 (10µm) RG786903 RG786907 RG786515 RG786525(S,S)-Whelk-01 3 (10µm) RG786902 RG786906 RG786615 RG786625$1076 $1076 $1913 $6575D-Leucine RG731607 RG731618 RG731054 RG731254L-Leucine RG731608 RG731619 RG731041 RG731241D-Phenylglycine RG731609 RG731620 RG731021 RG731221L-Phenylglycine RG731610 RG731621 RG731024 RG731224$1076 $1076 $1913 $6575(R,R)-Whelk-01 3 RG780255 RG780252 RG780201 RG780202(S,S)-Whelk-01 3 RG780155 RG780152 RG780101 RG780102- - $2033 $6575(R,R)-Whelk-02 3 (10µm) - - RG786315 RG786325(S,S)-Whelk-02 3 (10µm) - - RG786415 RG7864251 Bulk material and preparative columns available2 Other dimension columns available3 Based on Kromasil silicaFree Chiral Screening ServiceRegis offers a free chiral screening service where a smallamount of customer supplied sample (25mg) is screenedon the range of Regis Pirkle-Type columns. If you areinterested in this service please contactCanadian Life Science to obtain a submission form andConfidentiality Agreement.Advice can also be given on scaling up chiral separationsto preparative proportions.Regis Chiral LC Application GuideA wide range of examples of enantiomer separations can be found in the Regis Chiral LC Application Guide VI.Please contact Canadian Life Science to receive a copy.Toll Free: 1-888-226-2775 E-mail: info@lifescience.ca Web: www.lifescience.ca 219

APPLICATIONS - REGIS TECHNOLOGIES4(3H)-Quinazolone derivativesSulpiridePrilocaineMethadone hydrochlorideLansoprazoleTaxifolin220 Toll Free: 1-888-226-2775 E-mail: info@lifescience.ca Web: www.lifescience.ca