10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010Antioxidant activity <strong>of</strong> extracts <strong>of</strong> Helleborus odorusWaldst. & Kit, H. multifidus Vis. <strong>and</strong> H. hercegovinusMartinis.Čakara, J. 1 , Parić, A. 2 , Vidic, D. 3 , Maksimović, M. 3 , Bajrovića, K. 11 Institute for genetic engineering <strong>and</strong> biotechnology, Sarajevo, Bosna iHercegovina2 Department <strong>of</strong> biology, Faculty <strong>of</strong> Science, University <strong>of</strong> Sarajevo, Bosna iHercegovina3 Department <strong>of</strong> chemistry,Faculty <strong>of</strong> Science, University <strong>of</strong> Sarajevo, Bosnai Hercegovinajasmina.hindija@ingeb.baThis study was undertaken in order to evaluate possible antioxidative activity<strong>of</strong> three Helleborus taxa. Dry leaves <strong>and</strong> root <strong>of</strong> three Heleborus taxa were extractedwith ethanol <strong>and</strong> water. Phytochemical evaluati<strong>on</strong> <strong>of</strong> selected extracts was d<strong>on</strong>eusing spectrophotometric methods <strong>and</strong> 1,1-Diphenyl-2-picrylhydrazyl radical –scavenging activity (DPPH) assay was used for measuring antioxidative activity <strong>of</strong>extracts. Phytochemical evaluati<strong>on</strong> showed that leaves c<strong>on</strong>tain high level <strong>of</strong> totalphenolic <strong>and</strong> flav<strong>on</strong>oid c<strong>on</strong>tent. Results from <strong>the</strong> DPPH assay indicated that <strong>the</strong>activity <strong>of</strong> ethanol <strong>and</strong> water extract <strong>of</strong> leaves was higher than that <strong>of</strong> positivec<strong>on</strong>trol (thymol). Extracts from roots <strong>of</strong> H. odorus also displayed higher antioxidantactivity than positive probe, while H. mulifidus <strong>and</strong> H. hercegovinus roots extractswere less effective. The IC50 values varied from 0.25 mg/mL to 6.18 mg/mL.Statistically significant correlati<strong>on</strong> between total phenolic c<strong>on</strong>tent <strong>and</strong> antioxidativepr operties indicates that <strong>the</strong>se compounds c<strong>on</strong>tributed to antioxidant activity.97
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