10th Symposium on the Flora of Southeastern Serbia and ...

10th Symposium on the Flora of Southeastern Serbia and ... 10th Symposium on the Flora of Southeastern Serbia and ...

13.07.2015 Views

10 th ong>Symposiumong> on the Flora of Southeastern Serbia and Neighbouring regions,Vlasina 17 to 20 June 2010Effect of IBA on root formation in Beta vulgaris L.Tošić, S., Stojičić, D.,Mitrović, T.Department of Biology and Ecology, Faculty of Natural Science andMathematics, University of Niš, Serbiatosicsvetlana59@yahoo.comSugar beet is the major sucrose-producing crop grown in temperate zones.The aim of this study was to investigate the effect of different concentrations of IBAon root formation in few cultivars of Beta vulgaris L. After micropropagation,shoots of few cultivars of B. vulgaris L. were rooted on Gamborg media containing0.1, 0.5, 1.0 and 2.0 mg/l indole-3-butyric acid (IBA). The number of rootedplantlets and roots length were recorded at three DAIs (25, 25-44 and 44-45). Thetime of cultivation was also studied factor. Different concentrations of IBA hadsignificant effects on the examined parameters. The rooting ability increased withconcentrations of IBA. IBA at level 1.0 mg/l and 2.0 mg/l were found to be the besttreatment for rooting of micropropagated shoots. The percentage of rooted plantletsis higher for longer duration of treatment in culture. Key words: In vitro rootformation, Beta vulgaris L. IBA.Effect of IBA on root formation in Beta vulgaris L.Tošić, S. 1 , Stojičić, D.,Mitrović, T. 1 , Trajkovič, R. 21 Department of Biology and Ecology, Faculty of Natural Science andMathematics, University of Niš, Serbia2 Department of Biology, Faculty of Natural Science and Mathematics,University of Kosovska Mitrovica, Serbiatosicsvetlana59@yahoo.comSugar beet is the major sucrose-producing crop grown in temperate zones.The aim of this study was to investigate the effect of different concentrations of IBAon root formation in few cultivars of Beta vulgaris L. After micropropagation,shoots of few cultivars of B. vulgaris L. were rooted on Gamborg media containing0.1, 0.5, 1.0 and 2.0 mg/l indole-3-butyric acid (IBA). The number of rootedplantlets and roots length were recorded at three DAIs (25, 25-44 and 44-45). Thetime of cultivation was also studied factor. Different concentrations of IBA hadsignificant effects on the examined parameters. The rooting ability increased withconcentrations of IBA. IBA at level 1.0 mg/l and 2.0 mg/l were found to be the besttreatment for rooting of micropropagated shoots. The percentage of rooted plantletsis higher for longer duration of treatment in culture. Key words: In vitro rootformation, Beta vulgaris L. IBA.82

10 th ong>Symposiumong> on the Flora of Southeastern Serbia and Neighbouring regions,Vlasina 17 to 20 June 201010 th ong>Symposiumong> on the Flora ofSoutheastern Serbiaand Neighbouring regions,Vlasina 17 to 20 June 2010Phytochemistry and PhytotherapyFitohemija i fitoterapija83

10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010Effect <strong>of</strong> IBA <strong>on</strong> root formati<strong>on</strong> in Beta vulgaris L.Tošić, S., Stojičić, D.,Mitrović, T.Department <strong>of</strong> Biology <strong>and</strong> Ecology, Faculty <strong>of</strong> Natural Science <strong>and</strong>Ma<strong>the</strong>matics, University <strong>of</strong> Niš, <strong>Serbia</strong>tosicsvetlana59@yahoo.comSugar beet is <strong>the</strong> major sucrose-producing crop grown in temperate z<strong>on</strong>es.The aim <strong>of</strong> this study was to investigate <strong>the</strong> effect <strong>of</strong> different c<strong>on</strong>centrati<strong>on</strong>s <strong>of</strong> IBA<strong>on</strong> root formati<strong>on</strong> in few cultivars <strong>of</strong> Beta vulgaris L. After micropropagati<strong>on</strong>,shoots <strong>of</strong> few cultivars <strong>of</strong> B. vulgaris L. were rooted <strong>on</strong> Gamborg media c<strong>on</strong>taining0.1, 0.5, 1.0 <strong>and</strong> 2.0 mg/l indole-3-butyric acid (IBA). The number <strong>of</strong> rootedplantlets <strong>and</strong> roots length were recorded at three DAIs (25, 25-44 <strong>and</strong> 44-45). Thetime <strong>of</strong> cultivati<strong>on</strong> was also studied factor. Different c<strong>on</strong>centrati<strong>on</strong>s <strong>of</strong> IBA hadsignificant effects <strong>on</strong> <strong>the</strong> examined parameters. The rooting ability increased withc<strong>on</strong>centrati<strong>on</strong>s <strong>of</strong> IBA. IBA at level 1.0 mg/l <strong>and</strong> 2.0 mg/l were found to be <strong>the</strong> besttreatment for rooting <strong>of</strong> micropropagated shoots. The percentage <strong>of</strong> rooted plantletsis higher for l<strong>on</strong>ger durati<strong>on</strong> <strong>of</strong> treatment in culture. Key words: In vitro rootformati<strong>on</strong>, Beta vulgaris L. IBA.Effect <strong>of</strong> IBA <strong>on</strong> root formati<strong>on</strong> in Beta vulgaris L.Tošić, S. 1 , Stojičić, D.,Mitrović, T. 1 , Trajkovič, R. 21 Department <strong>of</strong> Biology <strong>and</strong> Ecology, Faculty <strong>of</strong> Natural Science <strong>and</strong>Ma<strong>the</strong>matics, University <strong>of</strong> Niš, <strong>Serbia</strong>2 Department <strong>of</strong> Biology, Faculty <strong>of</strong> Natural Science <strong>and</strong> Ma<strong>the</strong>matics,University <strong>of</strong> Kosovska Mitrovica, <strong>Serbia</strong>tosicsvetlana59@yahoo.comSugar beet is <strong>the</strong> major sucrose-producing crop grown in temperate z<strong>on</strong>es.The aim <strong>of</strong> this study was to investigate <strong>the</strong> effect <strong>of</strong> different c<strong>on</strong>centrati<strong>on</strong>s <strong>of</strong> IBA<strong>on</strong> root formati<strong>on</strong> in few cultivars <strong>of</strong> Beta vulgaris L. After micropropagati<strong>on</strong>,shoots <strong>of</strong> few cultivars <strong>of</strong> B. vulgaris L. were rooted <strong>on</strong> Gamborg media c<strong>on</strong>taining0.1, 0.5, 1.0 <strong>and</strong> 2.0 mg/l indole-3-butyric acid (IBA). The number <strong>of</strong> rootedplantlets <strong>and</strong> roots length were recorded at three DAIs (25, 25-44 <strong>and</strong> 44-45). Thetime <strong>of</strong> cultivati<strong>on</strong> was also studied factor. Different c<strong>on</strong>centrati<strong>on</strong>s <strong>of</strong> IBA hadsignificant effects <strong>on</strong> <strong>the</strong> examined parameters. The rooting ability increased withc<strong>on</strong>centrati<strong>on</strong>s <strong>of</strong> IBA. IBA at level 1.0 mg/l <strong>and</strong> 2.0 mg/l were found to be <strong>the</strong> besttreatment for rooting <strong>of</strong> micropropagated shoots. The percentage <strong>of</strong> rooted plantletsis higher for l<strong>on</strong>ger durati<strong>on</strong> <strong>of</strong> treatment in culture. Key words: In vitro rootformati<strong>on</strong>, Beta vulgaris L. IBA.82

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