10th Symposium on the Flora of Southeastern Serbia and ...

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10 th <strong>Symposium</strong> on the Flora of Southeastern Serbia and Neighbouring regions,Vlasina 17 to 20 June 2010status of seeds and lowered germination percentage, growth and flowering. In oldseeds, CAT and SOD activity, were significantly lower, and de novo proteinsynthesis started later during imbibition. Under the suitable photoperiodic conditionsin vitro, plant flowers in 15 days, and produces seeds in 10 weeks. C. rubrum plantsmodify their growth and development in accordance with photoperiod. We showedthat the growth pattern to the end of ontogenesis, the flowering and the seeddevelopment, are all determined by the photoperiod the seedlings experience duringearly phases of reproductive development - induction and evocation of flowering.We showed changes in antioxidative enzymes activities in different phases ofvegetative and reproductive development. The highest CAT activity was measuredat the time of flowering, PODs are involved in determination of C. rubrum growthand development in accordance with the environment and the absence of some SODisoforms could be the indicator of C. rubrum senescence.Diaporthe eres complex – Novi patogen Kantariona u SrbijiPavlović, S. 1 , Stojanović, S 2 , Starović, M. 2 , Stevic, T. 11 Institut za proučavanje lekovitog bilja „Dr Josif Pančić“ Beograd, Srbija2 Institut za zaštitu bilja i životnu sredinu, Beograd, Srbijaspavlovic@mocbilja.rsKantarion (Hypericum perforatum L.) je višegodišnja lekovita biljka koja seplantažno gaji u Srbij poslednjih sedam godina i na kojoj su do sada opisane četirivrste patogenih gljiva iz roda Fusarium i Colletotrichum gleosporoides (Pavlović etal, 2000; Ivanović i sar.,2000). Tokom 2008. godine zapaženo je novo oboljenje naplantažnim zasadima kantariona u lokalitetu Pančevo. Karakteristični simptomi sejavljaju na osušenom stablu u drugoj godini gajenja u vidu nekrotičnih pega nakojima se formira masa pojedinačnih piknida koji su u vlažnoj sredini produkovali αi ß konidije. α konidije su jednoćelične, ovalne sa zaobljenim krajevima, hialinske iimaju dve uljane kapi karakteristične za rod Phomopsis a ß konidije su jednoćelijske,hialinske, končaste izdužene. Korišćenjem standarnog fitopatološkog metodananošenjem fragmenta tkiva sa granice zdrave i obolele kore na hranljivu podloguod krompira (PDA) izvršena je izolacija patogena (Dhingra and Sinclair 1986).Porast patogena ispitivan je na PDA podlozi na temperaturi 25°C u uslovimanaizmeničnog osvetlenja (12/12 h svetlo/mrak). U ovakvim uslovim patogen razvijaretku belu, vunastu, ili pamučnu vazdušnu miceliju koja kasnije poprima nijanseprljavo bele boje. Nakon deset dana stvaraju se stromatične tvorevine u okviru kojihse obrazuju pojedinačne, ili u grupama razbacane tamne piknidije srednje veličine(1-3 mm). Identifikacija do roda vršena je na osnovu makroskopskih i odgajivačkihodlika patogena, a identifikacija vrste u Institute of Royal Netherlands Academy of78
10 th <strong>Symposium</strong> on the Flora of Southeastern Serbia and Neighbouring regions,Vlasina 17 to 20 June 2010Arts and Sciences, Fungal Biodiversity Centre, 2008. Ovo je prvi nalaz ove gljive naobolelim biljkama kantariona. Figure 3. Colony of Diaporthe eres complex Figure 4:Cirrhus, containing spores of Diaporthe eres complex Figure 5a: Lesion on St'Johansworth stem two weeks after inoculation Figure 5b: Cirrhi of Diaporthe eres complexon St'Johans worth stem three weeks after inoculation.Somatic embryogenesis and in vitro plantlet regenerationof Lilium martagon L. var. cattaniae Vis.Trbojević, S., Karalija, E., Muratović, E., Parić, A.Prirodno-matematički fakultet, Sarajevo, Bosna i Hercegovina,sanjatrbojević79@gmail.comIn this study we examined organogenic capacity of leaves and bulbs explantsof Lilium martagon L. var. cattaniae Vis. For induction of in vitro somaticembryogenesis and adventitive regeneration from those explants, differentconcentrations of 2,4-dichlorophenoxyacetic acid and 6-benzilaminopurine (from0,25 mg/l to 8,00 mg/l) added to MS basal medium were used. Our results indicatethat concentration of 0,5 mg/l 2,4-dichlorophenoxyacetic acid and 4 mg/l 6-benzilaminopurine promoted somatic embryogenesis from leaves of Liliummartagon var. cattaniae, while all other concentrations promoted direct shootregeneration from bulb explants. Root formation was induced on MS basal mediumwith 0,2 mg/l indole butyric acid. These plantlets were acclimatized well in agreenhouse conditions.Role of antioxidat enzymes in the seasonal adaptation ofPicea omorika (Pančić) PurkyneBogdanović Pristov, J., Mitrović, A., Spasojević, I., Radotić, K.Institut za multidisciplinarna istraživanja, Beograd, Srbijamala@imsi.rsWe studied seasonal variation in the activity and isoenzyme pattern ofperoxidase, catalase, catechol oxidase and superoxid-dismutase in the needles ofPicea omorika (Pančić) Purkyne trees. The samples were collected from the naturalhabitat of the species, Mt. Tara. This endemic coniferous species is exposed tosubfreezing temperatures that range from -10 to -30°C during the autumn/winter andhigh temperatures exceeding 30°C during the summer. Characteristic EPR signal offree or weakly bound Mn2+ was used as an indicator of oxidative status of needles,79
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