10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ... 10th Symposium on the Flora of Southeastern Serbia and ...
10 th
10 th
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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010Uticaj benzenskog ekstrakta sene (Cassia angustifoliaVahl.) na vijabilnost ćelija kostne srži mišaAleksić, M. 1 , Vasiljević, P. 1 , Najman, S. 2 , Vukelić, M. 2 , Manojlović N. 3 ,Živanov-Čurlis, J. 21 Odsek za biologiju i ekologiju, Prirodno-matematički fakultet, Univerzitet uNišu, Srbija2 Institut za biologiju sa humanom genetikom, Medicinski fakultet, Univerzitetu Nišu, Srbija3 Odsek za farmaciju Medicinski fakultet, Univerzitet u Kragujevcu, Srbijaperica@pmf.ni.ac.rsSena (Cassia angustifolia Vahl.) je biljka iz roda Cassia rasprostranjena utropskim područjima istočne Afrike, Arabije i Indije. U tradici<strong>on</strong>alnoj mediciniAzije koristise list i plod biljke kao laksativ. Ekstrakcijom lista sene po Soxhlet-udobijen je benzenski ekstrakt. U ovom radu ispitivan je uticaj različitihk<strong>on</strong>centracija (10 mg/ml, 7.5 mg/ml, 5 mg/ml, 2.5 mg/ml i 1.5 mg/ml) benzenskogekstrakta lista sene na vijabilnost ćelija kostne srži miša. Procena vijabilnosti vršenaje Trypan blue testom na 2, 4, 6 i 24 sata. Kao negativna k<strong>on</strong>trola korišćene su ćelijekoje su inkubirane u RPMI-u. Najveći uticaj ima k<strong>on</strong>centracija od 10 mg/ml, doknajmanja k<strong>on</strong>centracija od 1.5 mg/ml pokazuje najmanji uticaj na vijabilnost ćelijakostne srži.Ispitivanje uticaja metanolskog ekstrakta tri vrste rodaCassia (Cassia angustifolia, Cassia tora, Cassia siamea) navijabilnost HeLa ćelijaVasiljević, P. 1 , Najman, S. 2 , Manojlović, N. 3 , Aleksić, M. 1 , Jušković, M. 1 ,Vukelić, M. 21 Odsek za biologiju i ekologiju, Prirodno-matematički fakultet, Univerzitet uNišu, Srbija2 Institut za biologiju sa humanom genetikom, Medicinski fakultet, Univerzitetu Nišu, Srbija3 Odsek za farmaciju Medicinski fakultet, Univerzitet u Kragujevcu, Srbijaperica@pmf.ni.ac.rsListovi biljaka iz roda Cassia se koristi kao <strong>of</strong>icijalna droga i prirodnilaksativ u svetskim farmakopejama. Ekstrakcijom listova tri vrste sene (Cassiaangustifolia, Cassia tora, Cassia siamea) sa Soxhlet-ovim ekstraktorom dobijen jemetanolski ekstrakt, koji je korišćen za ispitivanje citotoksične aktivnosti.73