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10th Symposium on the Flora of Southeastern Serbia and ...

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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010The Family Boletaceae s.l. (Excluding Boletus) in <strong>the</strong>Republic <strong>of</strong> M<strong>on</strong>tenegroKasom, G.Institute for <strong>the</strong> Protecti<strong>on</strong> <strong>of</strong> Nature <strong>of</strong> M<strong>on</strong>tenegro, M<strong>on</strong>tenegrogkasom@t-com.meThe Family Boletaceae s.l. (Excluding Boletus) in <strong>the</strong> Republic <strong>of</strong>M<strong>on</strong>tenegro This paper c<strong>on</strong>tains results <strong>of</strong> systematic research into Boletaceae s.l.(excluding Boletus L.) in <strong>the</strong> Republic <strong>of</strong> M<strong>on</strong>tenegro. 45 species bel<strong>on</strong>ging to 18genera are presented. One genus <strong>and</strong> three species are reported for <strong>the</strong> first timefrom M<strong>on</strong>tenegro. For each species, <strong>the</strong> published <strong>and</strong> unpublished sources <strong>of</strong> dataare given, as well as <strong>the</strong> collecti<strong>on</strong>s in which <strong>the</strong> material is deposited. Also, <strong>the</strong>review <strong>of</strong> macr<strong>of</strong>ungi with nati<strong>on</strong>al <strong>and</strong> internati<strong>on</strong>al significance is given.Genetic <strong>and</strong> morphological diversity <strong>of</strong> closely relatedSphagnum angustifolium, S. fallax <strong>and</strong> S. flexuosumSzurdoki, E. 1 , Szövényi, P. 2 , Major, A. 11 Hungarian Natural History Museum, Budapest, Hungary2 University <strong>of</strong> Freiburg, Freiburg, Germanyszurdoki@bot.nhmus.huWe are investigating genetic <strong>and</strong> morphological diversity <strong>of</strong> Hungarian <strong>and</strong>European S. angustifolium, S. fallax <strong>and</strong> S. flexuosum populati<strong>on</strong>s. We are utilizingten microsatellite loci <strong>and</strong> five sequencing primer to evaluate <strong>the</strong> tax<strong>on</strong>omicvalue/rank <strong>of</strong> <strong>the</strong> investigated species. Morphological measurements are held <strong>on</strong>spreading <strong>and</strong> hanging branches, stem leaves, pendent <strong>and</strong> spreading branch leaves.At present we are in <strong>the</strong> process <strong>of</strong> generating microsatellite pr<strong>of</strong>iles for 200specimens distributed across <strong>the</strong> distributi<strong>on</strong> range <strong>of</strong> <strong>the</strong> species in Europe. Untilnow we analysed microsatellite loci <strong>on</strong> 100 samples, from 14 countries (next 100samples are in progress). Most markers showed high variability <strong>and</strong> thus appear tobe appropriate to study <strong>the</strong> relati<strong>on</strong>ship <strong>of</strong> <strong>the</strong> closely related species. Although <strong>the</strong>rewere alleles specific to each <strong>of</strong> <strong>the</strong> three species <strong>the</strong> majority <strong>of</strong> <strong>the</strong> alleles are sharedam<strong>on</strong>g <strong>the</strong>m c<strong>on</strong>firming <strong>the</strong>ir close genetic relatedness. To see whe<strong>the</strong>r accessi<strong>on</strong>s<strong>of</strong> <strong>the</strong> three described morphological species comprise distinct genetic entitiespairwise genetic distances were subjected to multivariate analysis (PCOA). ThePCOA suggest that two <strong>of</strong> <strong>the</strong> three pairwise species comparis<strong>on</strong> (S. fallax vs. S.flexuosum <strong>and</strong> S fallax vs. S. angustifolium) are well separated from each o<strong>the</strong>r21

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