10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010Pinus halepensis Miller. The study was c<strong>on</strong>ducted <strong>on</strong> fresh material, while checking<strong>on</strong> several occasi<strong>on</strong>s were made <strong>on</strong> <strong>the</strong> exccata. Macroscopic descripti<strong>on</strong> <strong>of</strong> <strong>the</strong>species <strong>and</strong> photo illustrati<strong>on</strong>s were performed in situ by reflex camera Nik<strong>on</strong> FG20<strong>and</strong> Nik<strong>on</strong> digital 100. Microscopic analysis was performed with <strong>the</strong> help <strong>of</strong> opticalmicroscope (Leica DMLS) while micro illustrati<strong>on</strong>s were made with <strong>the</strong> help <strong>of</strong>digital cameras (Dc300). Drawings <strong>of</strong> micro elements were performed using pen <strong>and</strong>ink, <strong>the</strong>n processed in Adobe Photoshop.The microscopoc preparati<strong>on</strong>s wereobserved in water, C<strong>on</strong>go red, Melzer's reagent (for observing amiloid reacti<strong>on</strong> <strong>of</strong>asci), Cott<strong>on</strong> blue, <strong>and</strong> Lactophenol (for observing ascospore ornamentati<strong>on</strong>). Size<strong>of</strong> ascospores are presented based <strong>on</strong> measurements <strong>of</strong> 30 ascospores from differentapo<strong>the</strong>cia. The material is preserved in <strong>the</strong> herbarium <strong>of</strong> Mycological Centre,Biotechnical Faculty in Podgorica. Comments <strong>on</strong> ecological <strong>and</strong> tax<strong>on</strong>omiccharacteristics are given.Kristali vrsta roda Trifolium L. – morfologija, distribucija istrukturaZorić, L., Luković, J., Merkulov, Lj.Univerzitet u Novom Sadu, PMF, Departman za biologiju i ekologiju, NoviSad, Srbijalana.zoric@dbe.uns.ac.rsIspitivanje morfologije, distribucije i strukture kristala u vegetativnim ireproduktivnim organima izvršeno je kod dvadeset vrsta roda Trifolium L.korišćenjem svetlosne i skening elektr<strong>on</strong>ske mikroskopije, kao i pomoću EnergyDispersive spektroskopije. Ustanovljeno je prisustvo pojedinačnih kristala kalcijumoksalata, prizmatičnog oblika, u svim analiziranim biljnim delovima. U listu,zaliscima i čašici kristali se nalaze u ćelijama parenhimske sare oko provodnihsnopića, dok su u lisnoj dršci, stablu i dršci cvasti prisutni u ćelijama sare iznadsklerenhimskih grupa uz floemski deo snopića. U zaliscima i čašici kristali se takođenalaze i u jednom sloju ćelija mez<strong>of</strong>ila. Na osnovu tipa, morfologije i distribucijekristala vrste roda Trifolium se međusobno ne razlikuju, te stoga analiziraniparametri nisu od taks<strong>on</strong>omskog značaja za ovaj rod. Primećeno je da su kristalimalobrojni ili čak odstvuju u organima gajenih vrsta ovog roda, što verova tno, uzostale histološke karakteristike, doprinosi većoj svarljivosti i kvalitetu krme.20
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