10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010Activity <strong>of</strong> catalase <strong>on</strong> Geranium macrorrhizum L. causedby fire <strong>on</strong> habitats <strong>of</strong> Vidlič MountainMarković, M. 1 , Stanković, M. 2 , Pavlović, D. 2 , Nešić, M. 1 , Trajković, R. 3 ,Stankov-Jovanović, V. 41 Department <strong>of</strong> Biology <strong>and</strong> Ecology, Faculty <strong>of</strong> Science <strong>and</strong> Ma<strong>the</strong>matics,University <strong>of</strong> Niš, <strong>Serbia</strong>2 Department <strong>of</strong> Biology <strong>and</strong> Ecology, Faculty <strong>of</strong> Science, University <strong>of</strong>Kragujevac, <strong>Serbia</strong>3 Department <strong>of</strong> Biology <strong>and</strong> Ecology, Faculty <strong>of</strong> Science <strong>and</strong> Ma<strong>the</strong>matics,University <strong>of</strong> Kosovska Mitrovica, <strong>Serbia</strong>4 Department <strong>of</strong> Chemistry, Faculty <strong>of</strong> Science <strong>and</strong> Ma<strong>the</strong>matics, University<strong>of</strong> Niš, <strong>Serbia</strong>marijam@pmf.ni.ac.rsIn this comparative study were determined <strong>the</strong> activity <strong>of</strong> catalase (EC1.11.1.6, H2O2:H2O2 oxidoreductase) enzyme <strong>on</strong> different parts <strong>of</strong> plant speciesGeranium macrorrhizum L. (Geraniaceae) from two habitats <strong>on</strong> Vidlic Mt. <strong>on</strong>Eastern <strong>Serbia</strong>. The first group <strong>of</strong> plant samples used for determinati<strong>on</strong>, wereobtained from <strong>the</strong> habitat that is affected by fire two years ago. Measuring werecarried out simultaneously in samples <strong>of</strong> <strong>the</strong> c<strong>on</strong>trol group <strong>of</strong> plants from habitatn<strong>on</strong>affected by fire. Determinati<strong>on</strong> <strong>of</strong> enzyme activity was carried out at <strong>the</strong> root,leaves <strong>and</strong> flowers <strong>of</strong> plant species G. macrorrhizum. Catalase activity wasmeasured using <strong>the</strong> gasometric method <strong>and</strong> <strong>the</strong> values obtained for <strong>the</strong> activity wereexpressed as ml <strong>of</strong> O2 for 3 minutes. Values obtained for <strong>the</strong> catalase activity <strong>on</strong>parts <strong>of</strong> plants from <strong>the</strong> habitat affected by fire ranged from 11.36 to 17.17 mlO2/3min. Values for activity in plant parts <strong>of</strong> <strong>the</strong> c<strong>on</strong>trol group <strong>of</strong> plant ranged from8.56 to 12.12 ml O2/3min. Presented results show a significant increase in catalaseactivity in individuals from habitats affected by fire in relati<strong>on</strong> to <strong>the</strong> c<strong>on</strong>trol group.Increased catalase activity is a c<strong>on</strong>sequence <strong>of</strong> oxidative stress caused by chemicalchanges in soil that generated by fire.102
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