10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010ekvivalenta rutina). Kod biljne vrste Teucrium m<strong>on</strong>tanum, van požarišta,detektovana je najveća redukci<strong>on</strong>a moć (59,30 μg/ml ekvivalenata askorbinskekiseline). Najveću vrednost „scavening“ antioksidantnog slobodno-radikalskogkapaciteta prema DPPH radikalu pokazuje ekstrakt biljke Satureja m<strong>on</strong>tana saopožarenog područja (15,42 %). Svi rezultati su prikazani za metanolne ekstraktek<strong>on</strong>centracije 1 mg/ml.Antimikrobna aktivnost metanolnih ekstrakata navedenih biljnih vrstaispitivana je disk difuzi<strong>on</strong>om metodom na sedam vrsta mikroorganizama:Salm<strong>on</strong>ella ab<strong>on</strong>y NCTC 6017, Pseudom<strong>on</strong>as aeruginosa ATCC 9027, Bacillussubtilis ATCC 6633, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC8739, C<strong>and</strong>ida albicans ATCC 10231 i Aspergillus niger ATCC 16404. Ekstraktbiljke Satureja m<strong>on</strong>tana, van požarišta, (246 μg po disku), pokazao je aktivnost nasve mikroorganizme, osim na B. subtilis i A. niger. Najbolje dejstvo pokazao je na S.ab<strong>on</strong>y (z<strong>on</strong>a inhibicije 20 mm) i C. albicans (z<strong>on</strong>a inhibicije 20 mm). EkstraktAcinos alpinus, sa lokaliteta požarišta, bio je aktivan na tri mikroorganizma (936 μgpo disku): S. ab<strong>on</strong>y, S. aureus i C. albicans, sa najboljim dejstvom na C. albicans(z<strong>on</strong>a inhibicije 21 mm).Biological effects <strong>of</strong> rosemary essential oil (Rosmarinus<strong>of</strong>ficinalis L.)Stojanović-Radić, Z. 1 , Nešić, M. 1 , Čomić, Lj. 2 , Radulović, N. 11 Faculty <strong>of</strong> Science <strong>and</strong> Ma<strong>the</strong>matics, Department <strong>of</strong> Biology <strong>and</strong> Ecology,University <strong>of</strong> Nis, <strong>Serbia</strong>2 Faculty <strong>of</strong> Science, Department <strong>of</strong> Biology <strong>and</strong> Ecology, University <strong>of</strong>Kragujevac, <strong>Serbia</strong>lab3zmm@yahoo.comRosemary is well known as a spice <strong>and</strong> widely used plant in ethnomedicineworldwide. In this paper, commercial essential oil <strong>of</strong> rosemary was tested forantimicrobial, cytotoxic activity toge<strong>the</strong>r with its effect <strong>on</strong> germinati<strong>on</strong>.Antimicrobial activity testing showed moderate effect to both G-positive <strong>and</strong> G-negative bacteria. In order to determine its effect to <strong>the</strong> cell wall, spectrophotometricanalysis was performed. It was determined that rosemary affects <strong>the</strong> cell wall <strong>of</strong>bacterial cell.Cytotoxic activity <strong>of</strong> Rosmarinus <strong>of</strong>ficinalis essential oil had been evaluated.As a plant object, germinative bulbs <strong>of</strong> Allium cepa were used. Cytotoxic activitythat corresp<strong>on</strong>ded to <strong>the</strong> c<strong>on</strong>centrati<strong>on</strong> <strong>of</strong> essential oil was determined. It had beennoticed that rosemary essential oil affected mitotic phase i.e. it significantly sloweddown <strong>the</strong> mitosis. Also, investigati<strong>on</strong> <strong>of</strong> rosemary essential oil's activity to100
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