10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
10th Symposium on the Flora of Southeastern Serbia and ...
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10 th <str<strong>on</strong>g>Symposium</str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Flora</strong> <strong>of</strong> Sou<strong>the</strong>astern <strong>Serbia</strong> <strong>and</strong> Neighbouring regi<strong>on</strong>s,Vlasina 17 to 20 June 2010Antimikrobna aktivnost metanolnih ekstrakata odabranihvrsta lišajeva familije ParmeliaceaeStojanović, I. 1 , Radulović, N. 2 , Jovanović, O. 2 , Petrović, G. 2 , Stojanović, G. 21 Department <strong>of</strong> Pharmacy, Faculty <strong>of</strong> Medicine, University <strong>of</strong> Nis, <strong>Serbia</strong>2 Department <strong>of</strong> Chemistry, Faculty <strong>of</strong> Natural Sciences <strong>and</strong> Ma<strong>the</strong>matics,University <strong>of</strong> Nis, <strong>Serbia</strong>igor.pfc@hotmail.comLichens have been used in folk medicine as astringents, t<strong>on</strong>ics <strong>and</strong> forpulm<strong>on</strong>ary disease. Scientific researches justified antibiotic, antiviral, antitumor,analgesic, <strong>and</strong> antipyretic properties <strong>of</strong> lichen’s extracts. In this work <strong>the</strong>antimicrobial activity <strong>of</strong> methanol extracts <strong>of</strong> four Parmeliaceae species:Hypogymnia physodes (L.) Nyl., Evernia prunastri (L.) Ach., Flavoparmeliacaperata (L.) Hale <strong>and</strong> Parmelia sulcata Taylor were determined. Material <strong>and</strong>methods: Extracts were prepared by extracti<strong>on</strong> <strong>of</strong> dry <strong>and</strong> ground lichens withmethanol <strong>on</strong> ambient temperature without exposure to direct sunlight (24 h). Theantimicrobial assay were performed using <strong>the</strong> disc diffusi<strong>on</strong> technique placing 40 μL<strong>of</strong> methanol soluti<strong>on</strong> <strong>of</strong> extract c<strong>on</strong>centrati<strong>on</strong> <strong>of</strong> 25mg/mL <strong>on</strong> discs (12.7 mmdiameter). Followed microorganisms were used: Gram pozitive bacteria(Enterococus sp, B. subtilllis, Sarcina lutea, Micrococus flavus, S. aureus, Cl.piogeneus ), Gram negative bacteria (E. coli, P. vulgaris, S. enteritidis, P.aeruginosa, K. pneum<strong>on</strong>ie) <strong>and</strong> fungi (A. niger <strong>and</strong> C. albicans). Results:Aglomerative cluster analysis (AHC) <strong>of</strong> obtained results can be summarized in <strong>the</strong>following : • H. physodes <strong>and</strong> E. prunastri extracts manifested str<strong>on</strong>gest activity(diameter <strong>of</strong> inhibiti<strong>on</strong> z<strong>on</strong>es ranged from 17 to 26 mm) than F. caperata <strong>and</strong> P.sulcata extracts (diameter <strong>of</strong> inhibiti<strong>on</strong> z<strong>on</strong>es ranged from 15 to 22 mm). • Based <strong>on</strong>microorganisms´ susceptibility towards extracts two groups were distinguished: <strong>the</strong>first c<strong>on</strong>sisting <strong>of</strong> Gram negatve bacteria (P. vulgaris <strong>and</strong> K. pneum<strong>on</strong>ie) <strong>and</strong> fungi(A. niger <strong>and</strong> C. albicans) <strong>and</strong> <strong>the</strong> sec<strong>on</strong>d group including all Gram positive bacteria<strong>and</strong> three Gram negative bacteria (E. coli, S. eneritidis <strong>and</strong> P. aeruginosa).C<strong>on</strong>clusi<strong>on</strong>: The examined extracts showed moderate <strong>and</strong> n<strong>on</strong>selective antibacterialactivity a bit lower <strong>the</strong>n activity <strong>of</strong> tetracycline st<strong>and</strong>ard. It is worthy <strong>of</strong> menti<strong>on</strong>ingthat H. physodes <strong>and</strong> E. prunastri extracts inhibited A. niger <strong>and</strong> C. albicans morethan nistatine st<strong>and</strong>ard which suggests <strong>the</strong>ir possible use as active ingredients inphyto-<strong>the</strong>rapeutics.98
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