water bug genus (Aphelocheirus) and species <strong>of</strong> Baetis rhodani. The British method isbased <strong>on</strong> identificati<strong>on</strong> <strong>of</strong> all genera <strong>of</strong> the family Chir<strong>on</strong>omidae (Ruse, 2000).Table 4. Selected <strong>methods</strong> determining particular organism groups to genus level.MethodBelgian Biotic Index (De Pauw and Vanhooren, 1983)and Biotic Sediment Index (De Pauw and Heylen,2001)Danish Stream Fauna Index (Skriver et al., 2000)Extended Biotic Index (Ghetti, 1997) and Indice aRapporto (Stoch, 1986)Quality Rating Scheme (McGarrigle et al., 1992)Chir<strong>on</strong>omid Pupal Exuviae Technique (Ruse, 2000)Determined generaall genera <strong>of</strong> the phylum/class/order Plathelminthes,Hirudinea, Mollusca, Plecoptera, Ephemeroptera,Od<strong>on</strong>ata, Megaloptera, HemipteraBrachyptera, Capnia, Leuctra, Isogenus, Isoperla,Isoptena, Perlodes, Prot<strong>on</strong>emura, Siph<strong>on</strong>operla,Limnius, Amphinemura, Taeniopteryx, Elmis, Elodes,Ancylus, Asellus, Chir<strong>on</strong>omus, Gammarus, Sialisall genera <strong>of</strong> the class/order Tricladia, Hirudinea,Plecoptera, Ephemeroptera, Od<strong>on</strong>ataLeuctra, Aphelocheirus, Rheotanytarsus, Gammarus,Baetis rhodani, Asellus, Chir<strong>on</strong>omusall genera <strong>of</strong> Chir<strong>on</strong>omidae93. The French Oligochaeta Index for Sediment Bioindicati<strong>on</strong> focuses <strong>on</strong> selectedorders to indicate the quality <strong>of</strong> watercourses combining achievement <strong>of</strong> preciseecological informati<strong>on</strong> through identificati<strong>on</strong> to species or species group level withlow-cost sample processing. In general, this approach allows applicati<strong>on</strong> <strong>of</strong> specificsampling techniques, ensuring maximum coverage <strong>of</strong> the group studied.94. It must be noted that in several assessment <strong>methods</strong> taxa are identified to lowerlevels than required to adequately compute the respective quality index.Record <strong>of</strong> abundance95. In general, there are two alternatives to indicate the abundance <strong>of</strong> particular taxa<strong>of</strong> benthic invertebrates found in the sample: (1) number <strong>of</strong> individuals per area, (2)abundance stated in ranges e.g. logarithmic, (3) abundance not recorded - notrecording is not an estimate <strong>of</strong> abundance.96. The opti<strong>on</strong> with the most substantial informati<strong>on</strong> c<strong>on</strong>tent is the specificati<strong>on</strong> <strong>of</strong> thenumber <strong>of</strong> individuals per area. Nearly 50 percent <strong>of</strong> macroinvertebrate <strong>methods</strong>record the abundance <strong>of</strong> individuals that way. In fact, purely quantitative data requirearea-related sampling procedures by means <strong>of</strong> quadrate samplers, grabs or similardevices. Since these requirements are <strong>on</strong>ly met by a few schemes, abundancestatements based <strong>on</strong> semi-quantitative hand-net sampling are in most cases <strong>of</strong>restricted reliability but they are cheap, practical and.effective.88
97. The use <strong>of</strong> range values to record individual occurrence allows the estimati<strong>on</strong> <strong>of</strong>taxa abundance. Compared to the first opti<strong>on</strong>, this alternative is time saving becausean exact count <strong>of</strong> individual organisms is not necessary. Different abundanceclassificati<strong>on</strong> schemes are in use, the most comm<strong>on</strong> are listed in Table 5.98. The 3-class scheme derives from the well-known publicati<strong>on</strong> <strong>of</strong> Pantle and Buck(1955) and is mostly used in eastern European countries applying the Saprobic Index(Bosnia-Herzegovina, Croatia, Slovenia).99. Two systems to classify abundance in a five-fold scheme exist in Europe: Theclasses <strong>of</strong> the British system are based <strong>on</strong> a logarithmic scale <strong>of</strong> organisms’abundance (Murray-Bligh, 1999). Different from this is the allocati<strong>on</strong> <strong>of</strong> classes in theQuality Rating System as applied by Bulgaria. Here, the presence <strong>of</strong> more than 100individuals <strong>of</strong> a certain tax<strong>on</strong> is assigned to the highest class. Hungary specifiesrelative taxa abundance <strong>of</strong> a sample (five metres secti<strong>on</strong> kicked for 15 minutes). Otherschemes do not specify numerical boundary values but provide <strong>on</strong>ly verbaldescripti<strong>on</strong>s <strong>of</strong> abundance classes (e.g. McGarrigle et al., 1992).100. In German watercourse bioassessment abundance is stated in seven classes.Established by Knöpp (1955) this classificati<strong>on</strong> has been included in the Germanstandard DIN 38 410 (1990, 2003): Determinati<strong>on</strong> <strong>of</strong> Saprobic Index <strong>of</strong> RunningWaters. Bey<strong>on</strong>d its broad applicati<strong>on</strong> in Germany, <strong>methods</strong> in Serbia-M<strong>on</strong>tenegro,Slovakia and Switzerland operate <strong>on</strong> the basis <strong>of</strong> a seven-class abundance scheme.101. Assessment <strong>methods</strong> operating <strong>on</strong> the basis <strong>of</strong> presence/absence data <strong>of</strong>macroinvertebrate taxa do not necessarily need to record taxa abundance. Many bioticindices like I.B.G.N. (France, Luxembourg), IBE (Italy), BMWP-ASPT (e.g. Cyprus,Portugal, Spain, United Kingdom) or BBI (Belgium) are not designed to includeabundance informati<strong>on</strong>. Their outputs may be biased by single organisms drifting intothe sample from upstream reaches. Therefore, the individual systems prescribe toinclude <strong>on</strong>ly taxa that exceed a certain threshold <strong>of</strong> abundance to avoid false results.89
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Institute for Environment and Susta
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CONTENTSBackground and purpose of t
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Background and purpose of the docum
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States and candidate countries. Inf
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classification, each of these being
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BIOLOGICAL QUALITY ELEMENT: PHYTOPL
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indicators, species lists, frequenc
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53. The identification and enumerat
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64. In general, this technique is t
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RIVER BIOLOGICAL QUALITY ELEMENT: F
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84. The development of specific sta
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practice guides for identification
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100. The information received from
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Evaluation of the suitability of cu
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group should update its primary fin
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ReferencesAFNOR (Association Franç
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EN ISO 8689-2 Water quality - Biolo
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Lazaridou-Dimitriadou, M., C. Kouko
- Page 37 and 38: Shannon, C.E. and W. Weaver, 1949.
- Page 39: Annex I: Composition of the Geograp
- Page 43 and 44: Annex III: River biological assessm
- Page 45 and 46: Annex IV: Analysis of lake biologic
- Page 47 and 48: Number of sampling stations10080%60
- Page 49 and 50: IT 90% acetone spectrophotometricPT
- Page 51 and 52: 10. The sampling depth and volume s
- Page 53 and 54: PTESFIIE5667-2/98 Romanianstandardi
- Page 55 and 56: Sampling stations%1008060402001 2-1
- Page 57 and 58: MACROPHYTES16. The aquatic Macrophy
- Page 59 and 60: Plants sampled per GIG1008060%40Eme
- Page 61 and 62: NO qualitativ method species number
- Page 63 and 64: indicators, species lists, frequenc
- Page 65 and 66: 26. The sampling frequency is varia
- Page 67: CEN/TC 230/WG 2/ TG 4 N28, 2 nd wor
- Page 70 and 71: programs are based only on the diat
- Page 72 and 73: 21. Some countries like France, Est
- Page 74 and 75: Table 1. European methods for monit
- Page 76 and 77: countries also covers Non-EU Member
- Page 78 and 79: 49. The Danish Stream Fauna Index i
- Page 80 and 81: Hungary58. Since 2002 a modificatio
- Page 82 and 83: Acidification Index, based on the s
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- Page 86 and 87: size of the net range between 250 t
- Page 90 and 91: Table 5. Common abundance classific
- Page 92 and 93: 108. Process Assessment focuses on
- Page 94 and 95: Austria120. MuLFA: Ecological Integ
- Page 96 and 97: Sweden126. Swedish fish Index: Appe
- Page 98 and 99: ut comparisons have been made with
- Page 100 and 101: seasons for sampling are summer and
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- Page 104 and 105: Consultation open to ECOSTAT &inter
- Page 106 and 107: Table 1. List of European standards
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- Page 110 and 111: development of typologySweden Yes,
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- Page 116 and 117: • An integrated holistic evaluati
- Page 118 and 119: • Phytoplankton: Yes; Clorophyll
- Page 120 and 121: • Macroalgae: No• Benthic inver
- Page 122 and 123: • Macrophytes: No• Macroalgae:
- Page 124: Mission of the JRCThe mission of th