Report on Harmonisation of freshwater biological methods

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Sampling frequencyNOMEECCEBAATAL12-67-12+120 20 40 60 80 100Figure 22. Benthic invertebrates sampling frequency in the GIGs.Sampling period%120100806040200AL AT BA CE EC ME NOWhole yearVegetation periodIce free periodWinterSpringSummerAutumnFigure 23 Benthic invertebrates sampling period in the GIGs.23. In Table 5 the sampling methods and metrics for benthic invertebrates arepresented. It shows that a wide number of sampling approaches and metrics are used.The collection of samples is performed by several different devices: grab, Eckmanskip, sticking cylinder, triangle bottom scraper and hand net. The mesh size varieswidely from 100µm to 670µm. When kick sampling is used time (1-3 minutes), meshsize (100-670µm) and habitat sampled (littoral in general or stones) are different.Some countries sampled in lake littoral, other the profundal and some in both lakezones (IE). Two countries make use of international sampling standard methods,Estonia and Romania. These standards are currently under revision.24. Every country uses a different combination of metrics, the following metrics areeither used alone or in combination: abundance and relative abundance, diversity62
indicators, species lists, frequency of occurrence of individual taxa, number of taxa,group ratios, average score per taxa, biotic score, biotic integrity index, saprobicindex, average score per taxon and ratio of littoral to profundal taxa.Table 5. Benthic invertebrates sampling and metrics used in different countries withinthe GIGs..GIG/CountrySampling MethodMetricDEFRIEGBPLLTEEEEALPINEsampling with grab or sticking cylinder; meshsize for sieving 0.2 mmEckman skip + strainerlittoral samples taken by kick sampling on stony abundance, frequency of occurrence,substrate for 2 minutes using 670µm mesh size; presence of indicator taxa, group ratios andpond net bag with rectangular frame (260mm diversitywide, 200mm high); profundal samples taken byEckman Grab (5 replicates) at deepest point oflake3 minute kick sample with Freshwater biotic score, number of taxa and averageBiological Association pond net mesh 100um score per taxon, log abundance estimates,indicator taxa.BALTICEkmann and Birdge sampler, samples from the dominance structuredepth of 20 - 50 m5 samples taken in 1/40 m2 area by special biotic integrity indexdevice for quantitative measurements and 1sample by triangle bottom scraper, which isdragged for 2-5 metresguidance of sampling of aquatic benthic macroinvertebrates(ISO 7828:1985),(ISOspecies composition, number of species8265:1988),(ISO 9391:1993),(ISO 8689-1:2000),(ISO 8689-1:2000).Borutsky or Zabolotsky bottom sampler;international standards: (ISO 7828:1985). (ISO8689-1:2000)(ISO 8689-2:2000).percentages of larger taxonomic groups,ASPT, EPT indexesCENTRALNL depending on substrate macroinvertebrates are no metric used for classification; Zebrasampled from stones (5 stones), or the substrate mussels: biovolume and number ofitself is sampled with a grab (Ekman samples sites0.15x0.15m), or tube (diameter ca. 0.1m) orhand net (mesh 0.5 mm).GB 1 minute kick/sweep species listBE littoral, standard netDE sampling stations in different depths (2-3, 5, 7, indicator taxa, abundance, group ratio:10, 15, 24 m) along one transect, mesh size: 405 littoral/profundal faunaµm; or with grab or sticking cylinder; mesh sizefor sieving 0.2 mm and 0,5 mm; or Ekman-Birge sampler, dredgeEASTERN CONTINENTALRO Knopp method; Pantle - Buck method; SR ISO density, indicator taxa, abundance, number5667-2/98of species, saprobic indexES grabber samplers species determination63
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Institute for Environment and Susta
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CONTENTSBackground and purpose of t
Page 5 and 6:
Background and purpose of the docum
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States and candidate countries. Inf
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classification, each of these being
Page 11 and 12: BIOLOGICAL QUALITY ELEMENT: PHYTOPL
Page 13 and 14: indicators, species lists, frequenc
Page 15 and 16: 53. The identification and enumerat
Page 17 and 18: 64. In general, this technique is t
Page 19 and 20: RIVER BIOLOGICAL QUALITY ELEMENT: F
Page 21 and 22: 84. The development of specific sta
Page 23 and 24: practice guides for identification
Page 25 and 26: 100. The information received from
Page 27 and 28: Evaluation of the suitability of cu
Page 29 and 30: group should update its primary fin
Page 31 and 32: ReferencesAFNOR (Association Franç
Page 33 and 34: EN ISO 8689-2 Water quality - Biolo
Page 35 and 36: Lazaridou-Dimitriadou, M., C. Kouko
Page 37 and 38: Shannon, C.E. and W. Weaver, 1949.
Page 39: Annex I: Composition of the Geograp
Page 43 and 44: Annex III: River biological assessm
Page 45 and 46: Annex IV: Analysis of lake biologic
Page 47 and 48: Number of sampling stations10080%60
Page 49 and 50: IT 90% acetone spectrophotometricPT
Page 51 and 52: 10. The sampling depth and volume s
Page 53 and 54: PTESFIIE5667-2/98 Romanianstandardi
Page 55 and 56: Sampling stations%1008060402001 2-1
Page 57 and 58: MACROPHYTES16. The aquatic Macrophy
Page 59 and 60: Plants sampled per GIG1008060%40Eme
Page 61: NO qualitativ method species number
Page 65 and 66: 26. The sampling frequency is varia
Page 67: CEN/TC 230/WG 2/ TG 4 N28, 2 nd wor
Page 70 and 71: programs are based only on the diat
Page 72 and 73: 21. Some countries like France, Est
Page 74 and 75: Table 1. European methods for monit
Page 76 and 77: countries also covers Non-EU Member
Page 78 and 79: 49. The Danish Stream Fauna Index i
Page 80 and 81: Hungary58. Since 2002 a modificatio
Page 82 and 83: Acidification Index, based on the s
Page 84 and 85: Identification is predominantly to
Page 86 and 87: size of the net range between 250 t
Page 88 and 89: water bug genus (Aphelocheirus) and
Page 90 and 91: Table 5. Common abundance classific
Page 92 and 93: 108. Process Assessment focuses on
Page 94 and 95: Austria120. MuLFA: Ecological Integ
Page 96 and 97: Sweden126. Swedish fish Index: Appe
Page 98 and 99: ut comparisons have been made with
Page 100 and 101: seasons for sampling are summer and
Page 102 and 103: 102
Page 104 and 105: Consultation open to ECOSTAT &inter
Page 106 and 107: Table 1. List of European standards
Page 108 and 109: 108
Page 110 and 111: development of typologySweden Yes,
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one or other option depends on the
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114
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• An integrated holistic evaluati
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• Phytoplankton: Yes; Clorophyll
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• Macroalgae: No• Benthic inver
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• Macrophytes: No• Macroalgae:
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Mission of the JRCThe mission of th
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Report on Harmonisation of freshwater biological methods

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